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Identification of novel immunogenic proteins of Helicobacter pylori by proteome technology.

Utt, Meeme LU ; Nilsson, Ingrid LU ; Ljungh, Åsa LU and Wadström, Torkel LU (2002) In Journal of Immunological Methods 259(1-2). p.1-10
Abstract
Cell surface proteins of the human gastric pathogen Helicobacter pylori, reference strain CCUG 17874, were extracted with acid glycine and fractionated by heparin affinity chromatography. The extracts were subsequently analysed using high-resolution two-dimensional gel electrophoresis (2-DE) and immunoblotting. Four proteins of low molecular masses (25-30 kDa) stained by Coomassie R-350, were identified by peptide ESI-MS/MS sequencing after in-gel tryptic digestion. The identified proteins were recognised by sera from H. pylori-infected patients. Two of them are now described for the first time as immunogenic proteins of which one protein was determined to be distinct from all H. pylori proteins previously described. In addition, the... (More)
Cell surface proteins of the human gastric pathogen Helicobacter pylori, reference strain CCUG 17874, were extracted with acid glycine and fractionated by heparin affinity chromatography. The extracts were subsequently analysed using high-resolution two-dimensional gel electrophoresis (2-DE) and immunoblotting. Four proteins of low molecular masses (25-30 kDa) stained by Coomassie R-350, were identified by peptide ESI-MS/MS sequencing after in-gel tryptic digestion. The identified proteins were recognised by sera from H. pylori-infected patients. Two of them are now described for the first time as immunogenic proteins of which one protein was determined to be distinct from all H. pylori proteins previously described. In addition, the specificity of the identified peptides was evaluated using both 1-D and 2-D immunoblotting against a panel of sera from patients with various bacterial infections. The present identification of highly specific antigens of H. pylori will encourage the improvement of serological diagnostic tests to diagnose and monitor H. pylori infection. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Two-Dimensional, Helicobacter Infections, Gel, Bacterial Outer Membrane Proteins, Electrophoresis, Proteome, Serologic Tests, Antigens, Immunoblotting
in
Journal of Immunological Methods
volume
259
issue
1-2
pages
1 - 10
publisher
Elsevier
external identifiers
  • wos:000173057100001
  • pmid:11730836
  • scopus:0036133117
ISSN
1872-7905
DOI
10.1016/S0022-1759(01)00476-8
language
English
LU publication?
yes
id
aefef637-7cd9-4012-8017-c0cfd2d4204d (old id 107041)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11730836&dopt=Abstract
date added to LUP
2007-07-10 14:34:07
date last changed
2017-01-01 07:13:03
@article{aefef637-7cd9-4012-8017-c0cfd2d4204d,
  abstract     = {Cell surface proteins of the human gastric pathogen Helicobacter pylori, reference strain CCUG 17874, were extracted with acid glycine and fractionated by heparin affinity chromatography. The extracts were subsequently analysed using high-resolution two-dimensional gel electrophoresis (2-DE) and immunoblotting. Four proteins of low molecular masses (25-30 kDa) stained by Coomassie R-350, were identified by peptide ESI-MS/MS sequencing after in-gel tryptic digestion. The identified proteins were recognised by sera from H. pylori-infected patients. Two of them are now described for the first time as immunogenic proteins of which one protein was determined to be distinct from all H. pylori proteins previously described. In addition, the specificity of the identified peptides was evaluated using both 1-D and 2-D immunoblotting against a panel of sera from patients with various bacterial infections. The present identification of highly specific antigens of H. pylori will encourage the improvement of serological diagnostic tests to diagnose and monitor H. pylori infection.},
  author       = {Utt, Meeme and Nilsson, Ingrid and Ljungh, Åsa and Wadström, Torkel},
  issn         = {1872-7905},
  keyword      = {Two-Dimensional,Helicobacter Infections,Gel,Bacterial Outer Membrane Proteins,Electrophoresis,Proteome,Serologic Tests,Antigens,Immunoblotting},
  language     = {eng},
  number       = {1-2},
  pages        = {1--10},
  publisher    = {Elsevier},
  series       = {Journal of Immunological Methods},
  title        = {Identification of novel immunogenic proteins of Helicobacter pylori by proteome technology.},
  url          = {http://dx.doi.org/10.1016/S0022-1759(01)00476-8},
  volume       = {259},
  year         = {2002},
}