Advanced

Protein expression analysis: from "tip of the iceberg" to a global method.

James, Peter LU (2001) In Disease Markers 17(4). p.235-246
Abstract
In this review I will describe the advances that have recently been made in "traditional" two-dimensional gel based protein expression analysis. A major jump has been made toward the automation of gel image analysis and comparison, one of the major bottlenecks in the analysis chain as well as the automation of spot excision and preparation for mass spectrometric analysis. Currently the gel-based "proteome mapping" approach is highly effective and 300 gels and over 10,000 spots a week can be analysed. Very recently, viable alternatives to the use of two-dimensional gel electrophoresis have emerged and these approaches are discussed here. In combination with the recently developed stable isotopic tagging methods for peptide quantitation and... (More)
In this review I will describe the advances that have recently been made in "traditional" two-dimensional gel based protein expression analysis. A major jump has been made toward the automation of gel image analysis and comparison, one of the major bottlenecks in the analysis chain as well as the automation of spot excision and preparation for mass spectrometric analysis. Currently the gel-based "proteome mapping" approach is highly effective and 300 gels and over 10,000 spots a week can be analysed. Very recently, viable alternatives to the use of two-dimensional gel electrophoresis have emerged and these approaches are discussed here. In combination with the recently developed stable isotopic tagging methods for peptide quantitation and new mass spectrometers, this emerging technology will be a rapid and highly effective alternative to gel-based methods with few of the latter's shortcomings. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Gel, Two-Dimensional : trends, Genome, Proteins : metabolism, Electrophoresis
in
Disease Markers
volume
17
issue
4
pages
235 - 246
publisher
IOS Press
external identifiers
  • wos:000173434400005
  • scopus:0035660059
ISSN
0278-0240
language
English
LU publication?
yes
id
91991fdd-9879-419d-a2e4-9dad1879321d (old id 107106)
alternative location
http://iospress.metapress.com/openurl.asp?genre=article&issn=0278-0240&volume=17&issue=4&spage=235
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11790891&dopt=Abstract
date added to LUP
2007-07-03 15:30:46
date last changed
2018-01-07 09:41:42
@article{91991fdd-9879-419d-a2e4-9dad1879321d,
  abstract     = {In this review I will describe the advances that have recently been made in "traditional" two-dimensional gel based protein expression analysis. A major jump has been made toward the automation of gel image analysis and comparison, one of the major bottlenecks in the analysis chain as well as the automation of spot excision and preparation for mass spectrometric analysis. Currently the gel-based "proteome mapping" approach is highly effective and 300 gels and over 10,000 spots a week can be analysed. Very recently, viable alternatives to the use of two-dimensional gel electrophoresis have emerged and these approaches are discussed here. In combination with the recently developed stable isotopic tagging methods for peptide quantitation and new mass spectrometers, this emerging technology will be a rapid and highly effective alternative to gel-based methods with few of the latter's shortcomings.},
  author       = {James, Peter},
  issn         = {0278-0240},
  keyword      = {Gel,Two-Dimensional : trends,Genome,Proteins : metabolism,Electrophoresis},
  language     = {eng},
  number       = {4},
  pages        = {235--246},
  publisher    = {IOS Press},
  series       = {Disease Markers},
  title        = {Protein expression analysis: from "tip of the iceberg" to a global method.},
  volume       = {17},
  year         = {2001},
}