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Microfluidic components for protein characterization.

Laurell, Thomas LU ; Marko-Varga, György LU ; Ekström, Simon LU ; Bengtsson, Martin LU and Nilsson, Johan LU (2001) In Journal of Biotechnology 82(2). p.75-161
Abstract
The use of microfluidic components to create an analytical toolbox for the very rapidly growing field of proteomics is described. This toolbox provides novel generic analytical solutions that are highly adaptable for analysis of various biomolecules, ranging from high to low abundant. The components are fabricated using silicon micromachining and consist of a microchip immobilised enzyme reactor (microIMER), a piezoelectric microdispenser and high-density nanovial target plates. This microtechnology based platform interfaces matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI TOF-MS) to a wide range of upstream sample handling and/or analytical techniques. Examples of applications such as rapid on-line... (More)
The use of microfluidic components to create an analytical toolbox for the very rapidly growing field of proteomics is described. This toolbox provides novel generic analytical solutions that are highly adaptable for analysis of various biomolecules, ranging from high to low abundant. The components are fabricated using silicon micromachining and consist of a microchip immobilised enzyme reactor (microIMER), a piezoelectric microdispenser and high-density nanovial target plates. This microtechnology based platform interfaces matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI TOF-MS) to a wide range of upstream sample handling and/or analytical techniques. Examples of applications such as rapid on-line digestion (12 s) and sample preparation of proteins, interfacing to capillary liquid chromatography (100 attomol sensitivity), and in-vial chemistry on femtomol amounts of sample are presented. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Miniaturization, Nanotechnology, Proteins : chemistry, Proteins : isolation & purification, Proteome, Silicon, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization : instrumentation, Equipment Design, Biotechnology : instrumentation, Bioreactors
in
Journal of Biotechnology
volume
82
issue
2
pages
75 - 161
publisher
Elsevier
external identifiers
  • scopus:0035717680
ISSN
1873-4863
language
English
LU publication?
yes
id
53f62be0-a81e-4e08-9281-078a8e6cf489 (old id 107193)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11916253&dopt=Abstract
date added to LUP
2007-07-03 15:53:00
date last changed
2018-10-07 04:30:40
@article{53f62be0-a81e-4e08-9281-078a8e6cf489,
  abstract     = {The use of microfluidic components to create an analytical toolbox for the very rapidly growing field of proteomics is described. This toolbox provides novel generic analytical solutions that are highly adaptable for analysis of various biomolecules, ranging from high to low abundant. The components are fabricated using silicon micromachining and consist of a microchip immobilised enzyme reactor (microIMER), a piezoelectric microdispenser and high-density nanovial target plates. This microtechnology based platform interfaces matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI TOF-MS) to a wide range of upstream sample handling and/or analytical techniques. Examples of applications such as rapid on-line digestion (12 s) and sample preparation of proteins, interfacing to capillary liquid chromatography (100 attomol sensitivity), and in-vial chemistry on femtomol amounts of sample are presented.},
  author       = {Laurell, Thomas and Marko-Varga, György and Ekström, Simon and Bengtsson, Martin and Nilsson, Johan},
  issn         = {1873-4863},
  keyword      = {Miniaturization,Nanotechnology,Proteins : chemistry,Proteins : isolation & purification,Proteome,Silicon,Spectrometry,Mass,Matrix-Assisted Laser Desorption-Ionization : instrumentation,Equipment Design,Biotechnology : instrumentation,Bioreactors},
  language     = {eng},
  number       = {2},
  pages        = {75--161},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {Microfluidic components for protein characterization.},
  volume       = {82},
  year         = {2001},
}