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Hormone-sensitive lipase is not required for cholesteryl ester hydrolysis in macrophages.

Contreras, Juan Antonio LU (2002) In Biochemical and Biophysical Research Communications 292(4). p.900-903
Abstract
Storage of cholesteryl esters in the cytoplasm of macrophages is one of the earliest and most ubiquitous event observed in the development of arteriosclerosis. Macrophages have an enormous capacity to uptake and store cholesterol in the form of cytosolic cholesteryl ester droplets. These stores are mobilized by the action of a neutral cholesteryl ester hydrolase (NCEH), producing free cholesterol that is either secreted to extracellular acceptors or reesterified. It has been proposed that hormone-sensitive lipase (HSL) is responsible for the NCEH activity in macrophages. The present work shows, however, that peritoneal macrophages from HSL null mice hydrolyze cytosolic stores of cholesteryl esters at a comparable rate to that of peritoneal... (More)
Storage of cholesteryl esters in the cytoplasm of macrophages is one of the earliest and most ubiquitous event observed in the development of arteriosclerosis. Macrophages have an enormous capacity to uptake and store cholesterol in the form of cytosolic cholesteryl ester droplets. These stores are mobilized by the action of a neutral cholesteryl ester hydrolase (NCEH), producing free cholesterol that is either secreted to extracellular acceptors or reesterified. It has been proposed that hormone-sensitive lipase (HSL) is responsible for the NCEH activity in macrophages. The present work shows, however, that peritoneal macrophages from HSL null mice hydrolyze cytosolic stores of cholesteryl esters at a comparable rate to that of peritoneal macrophages from wild-type mice, therefore demonstrating that HSL is not the main NCEH in macrophages. (Less)
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published
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keywords
Cholesterol Esterase : metabolism, Cholesterol Esterase : genetics, Cholesterol Esterase : deficiency, Cultured, Cells, Bucladesine : pharmacology, Western, Blotting, Arteriosclerosis : metabolism, Animal, Adipose Tissue : enzymology, Peritoneal : metabolism, Mice, Inbred Strains, Knockout, Cholesterol Esters : metabolism, Cytoplasm : metabolism, Hydrolysis, Macrophages, Peritoneal : drug effects
in
Biochemical and Biophysical Research Communications
volume
292
issue
4
pages
900 - 903
publisher
Elsevier
external identifiers
  • pmid:11944899
  • wos:000175171800018
  • scopus:0036297998
ISSN
1090-2104
DOI
10.1006/bbrc.2002.6757
language
English
LU publication?
yes
id
d492982a-bf71-49c3-acaa-81f5bbaf578d (old id 107621)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11944899&dopt=Abstract
date added to LUP
2007-07-11 12:36:05
date last changed
2017-02-05 04:26:17
@article{d492982a-bf71-49c3-acaa-81f5bbaf578d,
  abstract     = {Storage of cholesteryl esters in the cytoplasm of macrophages is one of the earliest and most ubiquitous event observed in the development of arteriosclerosis. Macrophages have an enormous capacity to uptake and store cholesterol in the form of cytosolic cholesteryl ester droplets. These stores are mobilized by the action of a neutral cholesteryl ester hydrolase (NCEH), producing free cholesterol that is either secreted to extracellular acceptors or reesterified. It has been proposed that hormone-sensitive lipase (HSL) is responsible for the NCEH activity in macrophages. The present work shows, however, that peritoneal macrophages from HSL null mice hydrolyze cytosolic stores of cholesteryl esters at a comparable rate to that of peritoneal macrophages from wild-type mice, therefore demonstrating that HSL is not the main NCEH in macrophages.},
  author       = {Contreras, Juan Antonio},
  issn         = {1090-2104},
  keyword      = {Cholesterol Esterase : metabolism,Cholesterol Esterase : genetics,Cholesterol Esterase : deficiency,Cultured,Cells,Bucladesine : pharmacology,Western,Blotting,Arteriosclerosis : metabolism,Animal,Adipose Tissue : enzymology,Peritoneal : metabolism,Mice,Inbred Strains,Knockout,Cholesterol Esters : metabolism,Cytoplasm : metabolism,Hydrolysis,Macrophages,Peritoneal : drug effects},
  language     = {eng},
  number       = {4},
  pages        = {900--903},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Hormone-sensitive lipase is not required for cholesteryl ester hydrolysis in macrophages.},
  url          = {http://dx.doi.org/10.1006/bbrc.2002.6757},
  volume       = {292},
  year         = {2002},
}