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Primed B cells present type-II collagen to T cells.

Holmdahl, Meirav LU ; Vestberg, Mikael LU and Holmdahl, Rikard LU (2002) In Scandinavian Journal of Immunology 55(4). p.382-389
Abstract
Development of type-II collagen (CII)-induced arthritis (CIA) is dependent on a T-cell mediated activation of autoreactive B cells. However, it is still unclear if B cells can present CII to T cells. To investigate the role of B cells as antigen-presenting cells (APCs) for CII, we purified B cells from lymph nodes of immunized and nonimmunized mice. These B cells were used as APC for antigen-specific T-cell hybridomas. B cells from naïve mice did present native, triple-helical, CII (nCII) but also ovalbumin (OVA) and denatured CII (dCII) to antigen-specific T-cell hybridomas. In addition, B cells primed with nCII or OVA, but not dCII, activated the antigen-specific T-cell hybridomas two to three times better than naïve B cells. We conclude... (More)
Development of type-II collagen (CII)-induced arthritis (CIA) is dependent on a T-cell mediated activation of autoreactive B cells. However, it is still unclear if B cells can present CII to T cells. To investigate the role of B cells as antigen-presenting cells (APCs) for CII, we purified B cells from lymph nodes of immunized and nonimmunized mice. These B cells were used as APC for antigen-specific T-cell hybridomas. B cells from naïve mice did present native, triple-helical, CII (nCII) but also ovalbumin (OVA) and denatured CII (dCII) to antigen-specific T-cell hybridomas. In addition, B cells primed with nCII or OVA, but not dCII, activated the antigen-specific T-cell hybridomas two to three times better than naïve B cells. We conclude that antigen-primed B cells have the capacity to process and present CII to primed T cells, and antigen-primed antigen-specific B cells are more efficient as APC than naïve B cells. We further conclude that B cells have the potential to play an important role as APC in the development of CIA. (Less)
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organization
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publication status
published
subject
keywords
B-Lymphocytes : physiology, Collagen Type II : immunology, Macrophages : physiology, Mice, Inbred C57BL, Support, Inbred DBA, Non-U.S. Gov't, T-Lymphocytes : immunology, Arthritis : etiology, Antigen-Presenting Cells : physiology, Antibodies : immunology, Animal
in
Scandinavian Journal of Immunology
volume
55
issue
4
pages
382 - 389
publisher
Wiley-Blackwell
external identifiers
  • wos:000175115000011
  • pmid:11967120
  • scopus:0036224790
ISSN
1365-3083
DOI
10.1046/j.1365-3083.2002.01071.x
language
English
LU publication?
yes
id
9a5c9918-f28d-42f7-9e4a-e617d47af020 (old id 107767)
date added to LUP
2007-07-13 12:15:57
date last changed
2017-01-01 06:40:35
@article{9a5c9918-f28d-42f7-9e4a-e617d47af020,
  abstract     = {Development of type-II collagen (CII)-induced arthritis (CIA) is dependent on a T-cell mediated activation of autoreactive B cells. However, it is still unclear if B cells can present CII to T cells. To investigate the role of B cells as antigen-presenting cells (APCs) for CII, we purified B cells from lymph nodes of immunized and nonimmunized mice. These B cells were used as APC for antigen-specific T-cell hybridomas. B cells from naïve mice did present native, triple-helical, CII (nCII) but also ovalbumin (OVA) and denatured CII (dCII) to antigen-specific T-cell hybridomas. In addition, B cells primed with nCII or OVA, but not dCII, activated the antigen-specific T-cell hybridomas two to three times better than naïve B cells. We conclude that antigen-primed B cells have the capacity to process and present CII to primed T cells, and antigen-primed antigen-specific B cells are more efficient as APC than naïve B cells. We further conclude that B cells have the potential to play an important role as APC in the development of CIA.},
  author       = {Holmdahl, Meirav and Vestberg, Mikael and Holmdahl, Rikard},
  issn         = {1365-3083},
  keyword      = {B-Lymphocytes : physiology,Collagen Type II : immunology,Macrophages : physiology,Mice,Inbred C57BL,Support,Inbred DBA,Non-U.S. Gov't,T-Lymphocytes : immunology,Arthritis : etiology,Antigen-Presenting Cells : physiology,Antibodies : immunology,Animal},
  language     = {eng},
  number       = {4},
  pages        = {382--389},
  publisher    = {Wiley-Blackwell},
  series       = {Scandinavian Journal of Immunology},
  title        = {Primed B cells present type-II collagen to T cells.},
  url          = {http://dx.doi.org/10.1046/j.1365-3083.2002.01071.x},
  volume       = {55},
  year         = {2002},
}