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Eosinophil infiltration and activation at the gastric ulcer margin in rats.

Mei, Qi; Korsgren, Magnus LU ; Erjefält, Jonas LU and Sundler, Frank LU (2002) In European Journal of Gastroenterology and Hepathology 14(5). p.503-511
Abstract
BACKGROUND : Recruitment and activation of eosinophils have been studied intensely in asthma and other allergic diseases. Less is known about the infiltration and behaviour of eosinophils during gastric ulcer healing. AIM : To examine the tissue infiltration and activation of eosinophils in the ulcer margin at different time points after ulcer induction (days 1-15). METHODS : Eosinophil peroxidase (EPO) staining and transmission electron microscopy (TEM) were used to observe eosinophil infiltration and activation in rats with acetic-acid-induced ulcer in the oxyntic mucosa. The distribution of macrophages was evaluated by immunocytochemistry using the macrophage-specific antibodies ED1 and ED2. RESULTS : There was a prominent increase in... (More)
BACKGROUND : Recruitment and activation of eosinophils have been studied intensely in asthma and other allergic diseases. Less is known about the infiltration and behaviour of eosinophils during gastric ulcer healing. AIM : To examine the tissue infiltration and activation of eosinophils in the ulcer margin at different time points after ulcer induction (days 1-15). METHODS : Eosinophil peroxidase (EPO) staining and transmission electron microscopy (TEM) were used to observe eosinophil infiltration and activation in rats with acetic-acid-induced ulcer in the oxyntic mucosa. The distribution of macrophages was evaluated by immunocytochemistry using the macrophage-specific antibodies ED1 and ED2. RESULTS : There was a prominent increase in eosinophils around the ulcer margin at day 1 after ulcer induction, which peaked at day 5. TEM revealed characteristic signs of eosinophil activation, including cytolysis and piecemeal degranulation. Eosinophil cytolysis was the major form of activation, seen most frequently at day 5. A few scattered apoptotic eosinophils could also be observed. In normal controls and sham-operated rats, activated eosinophils were detected rarely. The distribution pattern of infiltrated eosinophils closely resembled that of macrophages at the ulcer margin. However, in the central part of the granulation tissue (at day 5) only macrophages could be found. CONCLUSIONS : There is marked infiltration and signs of activation of eosinophils together with macrophages at the margin of newly formed ulcers. (Less)
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published
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keywords
Female, Gastric Mucosa : ultrastructure, Macrophage Activation : physiology, Microscopy, Electron, Rats, Sprague-Dawley, Stomach Ulcer : immunology, Stomach Ulcer : physiopathology, Support, Non-U.S. Gov't, Animal, Eosinophils : immunology, Eosinophils : physiology
in
European Journal of Gastroenterology and Hepathology
volume
14
issue
5
pages
503 - 511
publisher
Lippincott Williams & Wilkins
external identifiers
  • pmid:11984148
  • wos:000175709600007
  • scopus:0036097781
ISSN
1473-5687
language
English
LU publication?
yes
id
844da6b1-ef95-4d85-85e7-ccb2c6b3b448 (old id 107952)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11984148&dopt=Abstract
date added to LUP
2007-07-18 10:00:29
date last changed
2017-01-15 03:28:06
@article{844da6b1-ef95-4d85-85e7-ccb2c6b3b448,
  abstract     = {BACKGROUND : Recruitment and activation of eosinophils have been studied intensely in asthma and other allergic diseases. Less is known about the infiltration and behaviour of eosinophils during gastric ulcer healing. AIM : To examine the tissue infiltration and activation of eosinophils in the ulcer margin at different time points after ulcer induction (days 1-15). METHODS : Eosinophil peroxidase (EPO) staining and transmission electron microscopy (TEM) were used to observe eosinophil infiltration and activation in rats with acetic-acid-induced ulcer in the oxyntic mucosa. The distribution of macrophages was evaluated by immunocytochemistry using the macrophage-specific antibodies ED1 and ED2. RESULTS : There was a prominent increase in eosinophils around the ulcer margin at day 1 after ulcer induction, which peaked at day 5. TEM revealed characteristic signs of eosinophil activation, including cytolysis and piecemeal degranulation. Eosinophil cytolysis was the major form of activation, seen most frequently at day 5. A few scattered apoptotic eosinophils could also be observed. In normal controls and sham-operated rats, activated eosinophils were detected rarely. The distribution pattern of infiltrated eosinophils closely resembled that of macrophages at the ulcer margin. However, in the central part of the granulation tissue (at day 5) only macrophages could be found. CONCLUSIONS : There is marked infiltration and signs of activation of eosinophils together with macrophages at the margin of newly formed ulcers.},
  author       = {Mei, Qi and Korsgren, Magnus and Erjefält, Jonas and Sundler, Frank},
  issn         = {1473-5687},
  keyword      = {Female,Gastric Mucosa : ultrastructure,Macrophage Activation : physiology,Microscopy,Electron,Rats,Sprague-Dawley,Stomach Ulcer : immunology,Stomach Ulcer : physiopathology,Support,Non-U.S. Gov't,Animal,Eosinophils : immunology,Eosinophils : physiology},
  language     = {eng},
  number       = {5},
  pages        = {503--511},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {European Journal of Gastroenterology and Hepathology},
  title        = {Eosinophil infiltration and activation at the gastric ulcer margin in rats.},
  volume       = {14},
  year         = {2002},
}