Rapid DNA library construction for functional genomic and metagenomic screening
(2008) In Applied and Environmental Microbiology 74(5). p.1649-1652- Abstract
- A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1144726
- author
- Schmitz, Jonathan E ; Daniel, Anu ; Collin, Mattias LU ; Schuch, Raymond and Fischetti, Vincent A
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied and Environmental Microbiology
- volume
- 74
- issue
- 5
- pages
- 1649 - 1652
- publisher
- American Society for Microbiology
- external identifiers
-
- pmid:18083885
- scopus:40549144318
- ISSN
- 0099-2240
- DOI
- 10.1128/AEM.01864-07
- language
- English
- LU publication?
- no
- id
- 107b3865-d187-43ea-9878-a055552ca567 (old id 1144726)
- date added to LUP
- 2016-04-01 11:45:47
- date last changed
- 2022-04-05 04:39:13
@article{107b3865-d187-43ea-9878-a055552ca567, abstract = {{A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample.}}, author = {{Schmitz, Jonathan E and Daniel, Anu and Collin, Mattias and Schuch, Raymond and Fischetti, Vincent A}}, issn = {{0099-2240}}, language = {{eng}}, number = {{5}}, pages = {{1649--1652}}, publisher = {{American Society for Microbiology}}, series = {{Applied and Environmental Microbiology}}, title = {{Rapid DNA library construction for functional genomic and metagenomic screening}}, url = {{http://dx.doi.org/10.1128/AEM.01864-07}}, doi = {{10.1128/AEM.01864-07}}, volume = {{74}}, year = {{2008}}, }