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Amperometric determination of L-lactate based on entrapment of lactate oxidase on a transducer surface with a semi-permeable membrane using a SIRE technology based biosensor. Application: tomato paste and baby food.

Kriz, Kirstin LU ; Kraft, Linda; Krook, Margareta and Kriz, Dario (2002) In Journal of Agricultural and Food Chemistry 50(12). p.3419-3424
Abstract
Determination of dissolved L-lactate in tomato paste and baby food samples using a SIRE-based (sensors based on injection of the recognition element) biosensor is reported. The measuring principle is based on the use of a small amount of enzyme, which is injected into an internal delivery flow system and held in direct spatial contact with the amperometric transducer by the use of a semipermeable membrane. Measurements are based upon the reversible enzymatic conversion of L-lactate to pyruvate and hydrogen peroxide by lactate oxidase. Differential measurements are performed in which the samples are measured in the presence and absence of enzyme allowing for control over matrix interferences present in crude samples. The linear range... (More)
Determination of dissolved L-lactate in tomato paste and baby food samples using a SIRE-based (sensors based on injection of the recognition element) biosensor is reported. The measuring principle is based on the use of a small amount of enzyme, which is injected into an internal delivery flow system and held in direct spatial contact with the amperometric transducer by the use of a semipermeable membrane. Measurements are based upon the reversible enzymatic conversion of L-lactate to pyruvate and hydrogen peroxide by lactate oxidase. Differential measurements are performed in which the samples are measured in the presence and absence of enzyme allowing for control over matrix interferences present in crude samples. The linear range investigated for the determination of L-lactate in tomato paste and baby food was 0-0.1 mM using a lactate oxidase concentration of 22 U/mL. Samples were diluted with buffer prior to biosensor measurements. The L-lactate concentrations of the tomato paste and baby food were determined to be 1.02 +/- 0.02 mM and 2.51 +/- 0.10 mM, respectively, using the standard addition method. The repeatability for tomato paste and baby food measurements was 2.5% (RSD, n = 15) and 4.0% (RSD, n = 15) and the reproducibility was 13.0% (RSD, n = 45) and 3.0% (RSD, n = 45), respectively. The concentration of dissolved L-lactate can be used as a measure of freshness in the food industry. All biosensor measurements were compared with measurements from an established spectrophotometric assay (Boehringer Mannheim). It was found that the biosensor had good correlation with the spectrophotometric method. The biosensor gave 12% higher values for the tomato paste measurements and 2.5% higher values for the baby food measurements. However, a distinct advantage of the biosensor is that it can perform L-lactate measurements within 3 minutes, whereas the spectrophotometric assay requires a 35-minute measurement time. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Artificial, Membranes, Lactic Acid : analysis, Infant Food : analysis, Infant, Human, Fruit : chemistry, Food Industry : standards, Immobilized, Biosensing Techniques, Enzymes, Mixed Function Oxidases, Quality Control, Reproducibility of Results, Tomatoes : chemistry
in
Journal of Agricultural and Food Chemistry
volume
50
issue
12
pages
3419 - 3424
publisher
The American Chemical Society
external identifiers
  • pmid:12033805
  • wos:000175961100010
  • scopus:0037023949
ISSN
0021-8561
DOI
10.1021/jf0114942
language
English
LU publication?
yes
id
ae767999-331b-4d76-b572-0e83c2efac65 (old id 108558)
date added to LUP
2007-07-16 09:53:16
date last changed
2017-05-14 03:29:09
@article{ae767999-331b-4d76-b572-0e83c2efac65,
  abstract     = {Determination of dissolved L-lactate in tomato paste and baby food samples using a SIRE-based (sensors based on injection of the recognition element) biosensor is reported. The measuring principle is based on the use of a small amount of enzyme, which is injected into an internal delivery flow system and held in direct spatial contact with the amperometric transducer by the use of a semipermeable membrane. Measurements are based upon the reversible enzymatic conversion of L-lactate to pyruvate and hydrogen peroxide by lactate oxidase. Differential measurements are performed in which the samples are measured in the presence and absence of enzyme allowing for control over matrix interferences present in crude samples. The linear range investigated for the determination of L-lactate in tomato paste and baby food was 0-0.1 mM using a lactate oxidase concentration of 22 U/mL. Samples were diluted with buffer prior to biosensor measurements. The L-lactate concentrations of the tomato paste and baby food were determined to be 1.02 +/- 0.02 mM and 2.51 +/- 0.10 mM, respectively, using the standard addition method. The repeatability for tomato paste and baby food measurements was 2.5% (RSD, n = 15) and 4.0% (RSD, n = 15) and the reproducibility was 13.0% (RSD, n = 45) and 3.0% (RSD, n = 45), respectively. The concentration of dissolved L-lactate can be used as a measure of freshness in the food industry. All biosensor measurements were compared with measurements from an established spectrophotometric assay (Boehringer Mannheim). It was found that the biosensor had good correlation with the spectrophotometric method. The biosensor gave 12% higher values for the tomato paste measurements and 2.5% higher values for the baby food measurements. However, a distinct advantage of the biosensor is that it can perform L-lactate measurements within 3 minutes, whereas the spectrophotometric assay requires a 35-minute measurement time.},
  author       = {Kriz, Kirstin and Kraft, Linda and Krook, Margareta and Kriz, Dario},
  issn         = {0021-8561},
  keyword      = {Artificial,Membranes,Lactic Acid : analysis,Infant Food : analysis,Infant,Human,Fruit : chemistry,Food Industry : standards,Immobilized,Biosensing Techniques,Enzymes,Mixed Function Oxidases,Quality Control,Reproducibility of Results,Tomatoes : chemistry},
  language     = {eng},
  number       = {12},
  pages        = {3419--3424},
  publisher    = {The American Chemical Society},
  series       = {Journal of Agricultural and Food Chemistry},
  title        = {Amperometric determination of L-lactate based on entrapment of lactate oxidase on a transducer surface with a semi-permeable membrane using a SIRE technology based biosensor. Application: tomato paste and baby food.},
  url          = {http://dx.doi.org/10.1021/jf0114942},
  volume       = {50},
  year         = {2002},
}