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Evaluation of the tissue microarray technique for immunohistochemical analysis in rectal cancer.

Fernebro, Eva LU ; Dictor, Michael LU ; Bendahl, Pär-Ola LU ; Fernö, Mårten LU and Nilbert, Mef LU (2002) In Archives of Pathology 126(6). p.702-705
Abstract
BACKGROUND: Immunohistochemical staining for tumor-associated proteins is widely used for the identification of novel prognostic markers. Recently, a tissue-conserving, high-throughput technique, tissue microarray, has been introduced. This technique uses 0.6-mm tissue core biopsy specimens, 500 to 1000 of which are brought into a new paraffin array block, which can be sectioned up to 100 times. METHODS: We evaluated the tissue microarray technique for immunohistochemical analysis in 20 rectal cancers. Immunohistochemical staining was performed for the proliferation marker Ki-67 and the tumor suppressor protein p53 in whole tissue sections and in tissue core biopsy specimens. RESULTS: The whole tissue sections were assessed by counting all... (More)
BACKGROUND: Immunohistochemical staining for tumor-associated proteins is widely used for the identification of novel prognostic markers. Recently, a tissue-conserving, high-throughput technique, tissue microarray, has been introduced. This technique uses 0.6-mm tissue core biopsy specimens, 500 to 1000 of which are brought into a new paraffin array block, which can be sectioned up to 100 times. METHODS: We evaluated the tissue microarray technique for immunohistochemical analysis in 20 rectal cancers. Immunohistochemical staining was performed for the proliferation marker Ki-67 and the tumor suppressor protein p53 in whole tissue sections and in tissue core biopsy specimens. RESULTS: The whole tissue sections were assessed by counting all cells in 10 high-power fields (x40), which resulted in a mean fraction of Ki-67-expressing tumor cells of 0.81 (range, 0.54-1.0). p53 expression assessed in whole tissue sections showed nuclear staining in 15 (75%) of 20 rectal carcinomas. For the tissue microarray technique, a median of 3 (range, 3-5) 0.6-mm tissue core biopsy specimens were studied from each of the 20 tumor specimens. The tissue microarray method gave a mean Ki-67 expression of 0.85 (range, 0.50-1.0) in tumor cell nuclei and showed p53 protein expression in the same 15 of 20 tumors as in the whole tissue sections. CONCLUSION: We conclude that the tissue microarray technique for immunohistochemical staining in rectal cancer yields staining of good quality and expression data for Ki-67 and p53 comparable to those obtained with whole tissue staining. The feasibility of tissue microarray thus enables time- and tissue-preserving studies of multiple markers in large tumor series. (Less)
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keywords
Rectal Neoplasms : chemistry, Protein p53 : analysis, Middle Age, Male, Ki-67 Antigen : analysis, Immunoenzyme Techniques, Human, Histocytological Preparation Techniques : methods, Female, Cell Count, Carcinoma : pathology, Carcinoma : chemistry, Needle, Biopsy, Adult, Aged, Rectal Neoplasms : pathology, Support, Non-U.S. Gov't, Tumor Markers, Biological : analysis
in
Archives of Pathology
volume
126
issue
6
pages
702 - 705
publisher
College Amer Pathologists
external identifiers
  • wos:000176056400011
  • pmid:12033959
  • scopus:0036269099
ISSN
0003-9985
language
English
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yes
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488ac96e-0540-4eca-b987-516a215578ad (old id 108564)
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http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12033959&dopt=Abstract
date added to LUP
2007-07-18 16:08:50
date last changed
2017-09-03 04:34:31
@article{488ac96e-0540-4eca-b987-516a215578ad,
  abstract     = {BACKGROUND: Immunohistochemical staining for tumor-associated proteins is widely used for the identification of novel prognostic markers. Recently, a tissue-conserving, high-throughput technique, tissue microarray, has been introduced. This technique uses 0.6-mm tissue core biopsy specimens, 500 to 1000 of which are brought into a new paraffin array block, which can be sectioned up to 100 times. METHODS: We evaluated the tissue microarray technique for immunohistochemical analysis in 20 rectal cancers. Immunohistochemical staining was performed for the proliferation marker Ki-67 and the tumor suppressor protein p53 in whole tissue sections and in tissue core biopsy specimens. RESULTS: The whole tissue sections were assessed by counting all cells in 10 high-power fields (x40), which resulted in a mean fraction of Ki-67-expressing tumor cells of 0.81 (range, 0.54-1.0). p53 expression assessed in whole tissue sections showed nuclear staining in 15 (75%) of 20 rectal carcinomas. For the tissue microarray technique, a median of 3 (range, 3-5) 0.6-mm tissue core biopsy specimens were studied from each of the 20 tumor specimens. The tissue microarray method gave a mean Ki-67 expression of 0.85 (range, 0.50-1.0) in tumor cell nuclei and showed p53 protein expression in the same 15 of 20 tumors as in the whole tissue sections. CONCLUSION: We conclude that the tissue microarray technique for immunohistochemical staining in rectal cancer yields staining of good quality and expression data for Ki-67 and p53 comparable to those obtained with whole tissue staining. The feasibility of tissue microarray thus enables time- and tissue-preserving studies of multiple markers in large tumor series.},
  author       = {Fernebro, Eva and Dictor, Michael and Bendahl, Pär-Ola and Fernö, Mårten and Nilbert, Mef},
  issn         = {0003-9985},
  keyword      = {Rectal Neoplasms : chemistry,Protein p53 : analysis,Middle Age,Male,Ki-67 Antigen : analysis,Immunoenzyme Techniques,Human,Histocytological Preparation Techniques : methods,Female,Cell Count,Carcinoma : pathology,Carcinoma : chemistry,Needle,Biopsy,Adult,Aged,Rectal Neoplasms : pathology,Support,Non-U.S. Gov't,Tumor Markers,Biological : analysis},
  language     = {eng},
  number       = {6},
  pages        = {702--705},
  publisher    = {College Amer Pathologists},
  series       = {Archives of Pathology},
  title        = {Evaluation of the tissue microarray technique for immunohistochemical analysis in rectal cancer.},
  volume       = {126},
  year         = {2002},
}