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Evaluation of the tissue microarray technique for immunohistochemical analysis in rectal cancer.

Fernebro, Eva LU ; Dictor, Michael LU ; Bendahl, Pär-Ola LU ; Fernö, Mårten LU and Nilbert, Mef LU (2002) In Archives of pathology & laboratory medicine 126(6). p.702-705
Abstract
BACKGROUND: Immunohistochemical staining for tumor-associated proteins is widely used for the identification of novel prognostic markers. Recently, a tissue-conserving, high-throughput technique, tissue microarray, has been introduced. This technique uses 0.6-mm tissue core biopsy specimens, 500 to 1000 of which are brought into a new paraffin array block, which can be sectioned up to 100 times. METHODS: We evaluated the tissue microarray technique for immunohistochemical analysis in 20 rectal cancers. Immunohistochemical staining was performed for the proliferation marker Ki-67 and the tumor suppressor protein p53 in whole tissue sections and in tissue core biopsy specimens. RESULTS: The whole tissue sections were assessed by counting all... (More)
BACKGROUND: Immunohistochemical staining for tumor-associated proteins is widely used for the identification of novel prognostic markers. Recently, a tissue-conserving, high-throughput technique, tissue microarray, has been introduced. This technique uses 0.6-mm tissue core biopsy specimens, 500 to 1000 of which are brought into a new paraffin array block, which can be sectioned up to 100 times. METHODS: We evaluated the tissue microarray technique for immunohistochemical analysis in 20 rectal cancers. Immunohistochemical staining was performed for the proliferation marker Ki-67 and the tumor suppressor protein p53 in whole tissue sections and in tissue core biopsy specimens. RESULTS: The whole tissue sections were assessed by counting all cells in 10 high-power fields (x40), which resulted in a mean fraction of Ki-67-expressing tumor cells of 0.81 (range, 0.54-1.0). p53 expression assessed in whole tissue sections showed nuclear staining in 15 (75%) of 20 rectal carcinomas. For the tissue microarray technique, a median of 3 (range, 3-5) 0.6-mm tissue core biopsy specimens were studied from each of the 20 tumor specimens. The tissue microarray method gave a mean Ki-67 expression of 0.85 (range, 0.50-1.0) in tumor cell nuclei and showed p53 protein expression in the same 15 of 20 tumors as in the whole tissue sections. CONCLUSION: We conclude that the tissue microarray technique for immunohistochemical staining in rectal cancer yields staining of good quality and expression data for Ki-67 and p53 comparable to those obtained with whole tissue staining. The feasibility of tissue microarray thus enables time- and tissue-preserving studies of multiple markers in large tumor series. (Less)
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; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Rectal Neoplasms : chemistry, Protein p53 : analysis, Middle Age, Male, Ki-67 Antigen : analysis, Immunoenzyme Techniques, Human, Histocytological Preparation Techniques : methods, Female, Cell Count, Carcinoma : pathology, Carcinoma : chemistry, Needle, Biopsy, Adult, Aged, Rectal Neoplasms : pathology, Support, Non-U.S. Gov't, Tumor Markers, Biological : analysis
in
Archives of pathology & laboratory medicine
volume
126
issue
6
pages
702 - 705
publisher
College of American Pathologists
external identifiers
  • wos:000176056400011
  • pmid:12033959
  • scopus:0036269099
ISSN
0003-9985
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000), Oncology, MV (013035000)
id
488ac96e-0540-4eca-b987-516a215578ad (old id 108564)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12033959&dopt=Abstract
date added to LUP
2016-04-01 16:13:35
date last changed
2022-01-28 18:12:13
@article{488ac96e-0540-4eca-b987-516a215578ad,
  abstract     = {{BACKGROUND: Immunohistochemical staining for tumor-associated proteins is widely used for the identification of novel prognostic markers. Recently, a tissue-conserving, high-throughput technique, tissue microarray, has been introduced. This technique uses 0.6-mm tissue core biopsy specimens, 500 to 1000 of which are brought into a new paraffin array block, which can be sectioned up to 100 times. METHODS: We evaluated the tissue microarray technique for immunohistochemical analysis in 20 rectal cancers. Immunohistochemical staining was performed for the proliferation marker Ki-67 and the tumor suppressor protein p53 in whole tissue sections and in tissue core biopsy specimens. RESULTS: The whole tissue sections were assessed by counting all cells in 10 high-power fields (x40), which resulted in a mean fraction of Ki-67-expressing tumor cells of 0.81 (range, 0.54-1.0). p53 expression assessed in whole tissue sections showed nuclear staining in 15 (75%) of 20 rectal carcinomas. For the tissue microarray technique, a median of 3 (range, 3-5) 0.6-mm tissue core biopsy specimens were studied from each of the 20 tumor specimens. The tissue microarray method gave a mean Ki-67 expression of 0.85 (range, 0.50-1.0) in tumor cell nuclei and showed p53 protein expression in the same 15 of 20 tumors as in the whole tissue sections. CONCLUSION: We conclude that the tissue microarray technique for immunohistochemical staining in rectal cancer yields staining of good quality and expression data for Ki-67 and p53 comparable to those obtained with whole tissue staining. The feasibility of tissue microarray thus enables time- and tissue-preserving studies of multiple markers in large tumor series.}},
  author       = {{Fernebro, Eva and Dictor, Michael and Bendahl, Pär-Ola and Fernö, Mårten and Nilbert, Mef}},
  issn         = {{0003-9985}},
  keywords     = {{Rectal Neoplasms : chemistry; Protein p53 : analysis; Middle Age; Male; Ki-67 Antigen : analysis; Immunoenzyme Techniques; Human; Histocytological Preparation Techniques : methods; Female; Cell Count; Carcinoma : pathology; Carcinoma : chemistry; Needle; Biopsy; Adult; Aged; Rectal Neoplasms : pathology; Support; Non-U.S. Gov't; Tumor Markers; Biological : analysis}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{702--705}},
  publisher    = {{College of American Pathologists}},
  series       = {{Archives of pathology & laboratory medicine}},
  title        = {{Evaluation of the tissue microarray technique for immunohistochemical analysis in rectal cancer.}},
  url          = {{http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12033959&dopt=Abstract}},
  volume       = {{126}},
  year         = {{2002}},
}