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Molecular identification and developmental expression of UV and green opsin mRNAs in the pineal organ of the Atlantic halibut.

Forsell, Johan LU ; Holmqvist, Bo LU and Ekström, Peter LU (2002) In Developmental Brain Research 136(1). p.51-62
Abstract
The pineal organ is the only differentiated photoreceptor organ present in embryos and early larvae of the Atlantic halibut (Hippoglossus hippoglossus). We investigated the molecular identity of opsins in the pineal organ, and their expression during different life stages. Using RT-PCR we identified two 681-bp gene sequences, named HPO1 and HPO4, in cDNA from adult pineal and whole embryos. The predicted amino acid sequences showed highest identity to the transmembrane regions of teleostean RH2 green cone opsins (HPO1, 72-91%) and SWS-1 UV cone opsins (HPO4, 71-83%). In situ hybridization revealed expression of HPO1 and HPO4 mRNA transcripts in photoreceptors in the pineal organ of embryos, larvae and adults. HPO1 and HPO4 mRNA transcripts... (More)
The pineal organ is the only differentiated photoreceptor organ present in embryos and early larvae of the Atlantic halibut (Hippoglossus hippoglossus). We investigated the molecular identity of opsins in the pineal organ, and their expression during different life stages. Using RT-PCR we identified two 681-bp gene sequences, named HPO1 and HPO4, in cDNA from adult pineal and whole embryos. The predicted amino acid sequences showed highest identity to the transmembrane regions of teleostean RH2 green cone opsins (HPO1, 72-91%) and SWS-1 UV cone opsins (HPO4, 71-83%). In situ hybridization revealed expression of HPO1 and HPO4 mRNA transcripts in photoreceptors in the pineal organ of embryos, larvae and adults. HPO1 and HPO4 mRNA transcripts were also expressed in the larval retina. Our study provides molecular evidence for short and middle wavelength light sensitive photoreceptors in the pineal organ of Atlantic halibut throughout life, and suggests that pineal photoreception may play an important role during embryonic and larval life stages, especially at the time when the retina does not possesses corresponding photoreceptor capacity. (Less)
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organization
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Contribution to journal
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published
subject
keywords
Animal, RNA, Messenger : analysis, Pineal Body : physiology, Pineal Body : growth & development, Photoreceptors, Vertebrate : physiology, Opsin : genetics, Molecular Sequence Data, Membrane Proteins : physiology, Membrane Proteins : genetics, In Situ Hybridization, Developmental, Gene Expression Regulation, Non-U.S. Gov't, Support, Flounder : genetics, Molecular, Cloning, Atlantic Ocean, Amino Acid Sequence
in
Developmental Brain Research
volume
136
issue
1
pages
51 - 62
publisher
Elsevier
external identifiers
  • wos:000176481800006
  • pmid:12036517
  • scopus:0037198592
ISSN
0165-3806
DOI
10.1016/S0165-3806(02)00351-6
language
English
LU publication?
yes
id
e96d9151-0013-4689-8f04-04f9b04bd9dd (old id 108593)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12036517&dopt=Abstract
date added to LUP
2007-07-19 15:34:24
date last changed
2017-04-23 04:25:01
@article{e96d9151-0013-4689-8f04-04f9b04bd9dd,
  abstract     = {The pineal organ is the only differentiated photoreceptor organ present in embryos and early larvae of the Atlantic halibut (Hippoglossus hippoglossus). We investigated the molecular identity of opsins in the pineal organ, and their expression during different life stages. Using RT-PCR we identified two 681-bp gene sequences, named HPO1 and HPO4, in cDNA from adult pineal and whole embryos. The predicted amino acid sequences showed highest identity to the transmembrane regions of teleostean RH2 green cone opsins (HPO1, 72-91%) and SWS-1 UV cone opsins (HPO4, 71-83%). In situ hybridization revealed expression of HPO1 and HPO4 mRNA transcripts in photoreceptors in the pineal organ of embryos, larvae and adults. HPO1 and HPO4 mRNA transcripts were also expressed in the larval retina. Our study provides molecular evidence for short and middle wavelength light sensitive photoreceptors in the pineal organ of Atlantic halibut throughout life, and suggests that pineal photoreception may play an important role during embryonic and larval life stages, especially at the time when the retina does not possesses corresponding photoreceptor capacity.},
  author       = {Forsell, Johan and Holmqvist, Bo and Ekström, Peter},
  issn         = {0165-3806},
  keyword      = {Animal,RNA,Messenger : analysis,Pineal Body : physiology,Pineal Body : growth & development,Photoreceptors,Vertebrate : physiology,Opsin : genetics,Molecular Sequence Data,Membrane Proteins : physiology,Membrane Proteins : genetics,In Situ Hybridization,Developmental,Gene Expression Regulation,Non-U.S. Gov't,Support,Flounder : genetics,Molecular,Cloning,Atlantic Ocean,Amino Acid Sequence},
  language     = {eng},
  number       = {1},
  pages        = {51--62},
  publisher    = {Elsevier},
  series       = {Developmental Brain Research},
  title        = {Molecular identification and developmental expression of UV and green opsin mRNAs in the pineal organ of the Atlantic halibut.},
  url          = {http://dx.doi.org/10.1016/S0165-3806(02)00351-6},
  volume       = {136},
  year         = {2002},
}