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Protective effect of low molecular weight heparin on experimental colitis: role of neutrophil recruitment and TNF-alpha production.

Wan, M X; Liu, Qing LU ; Wang, Yusheng LU and Thorlacius, Henrik LU (2002) In Inflammation Research1995-01-01+01:00 51(4). p.182-187
Abstract
OBJECTIVE: The purpose of this study was to examine the impact and mechanism of action of low molecular weight heparin (LMWH) in a model of murine colitis. MATERIALS AND METHODS: Balb/c mice were exposed to 5% dextran sodium sulfate (DSS) in the drinking water for five days. LMWH (500 units/kg/day) was administered by subcutaneous injection prior to and throughout the treatment period with DSS. Clinical disease activity index (DAI), including body weight loss, stool consistency and blood in feces were examined daily. Moreover, crypt height (CH), mucosal damage score (MDS), myeloperoxidase (MPO) activity and tumor necrosis factor-alpha (TNF-alpha) content in the colon were determined. RESULTS: DSS increased DAI, MDS, MPO activity and... (More)
OBJECTIVE: The purpose of this study was to examine the impact and mechanism of action of low molecular weight heparin (LMWH) in a model of murine colitis. MATERIALS AND METHODS: Balb/c mice were exposed to 5% dextran sodium sulfate (DSS) in the drinking water for five days. LMWH (500 units/kg/day) was administered by subcutaneous injection prior to and throughout the treatment period with DSS. Clinical disease activity index (DAI), including body weight loss, stool consistency and blood in feces were examined daily. Moreover, crypt height (CH), mucosal damage score (MDS), myeloperoxidase (MPO) activity and tumor necrosis factor-alpha (TNF-alpha) content in the colon were determined. RESULTS: DSS increased DAI, MDS, MPO activity and TNF-alpha production and decreased CH. Administration of LMWH markedly reduced DAI, MDS and reversed the CH-reduction. Moreover, in LMWH-treated animals, the MPO activity was reduced by more than 67% whereas mucosal levels of TNF-alpha was similar compared to DSS control mice. CONCLUSIONS: These findings suggest that LMWH inhibits murine colitis by interference with neutrophil recruitment and that LMWH may provide a novel pharmacological approach to treatment of inflammatory bowel disease. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Colitis, Leukocyte, Low molecular weight heparin, TNF-α
in
Inflammation Research1995-01-01+01:00
volume
51
issue
4
pages
182 - 187
publisher
Birkhaüser
external identifiers
  • wos:000175668700003
  • pmid:12058955
ISSN
1420-908X
language
English
LU publication?
yes
id
6f0184d5-9a53-4ec5-b40a-29be3aeedaf3 (old id 108724)
alternative location
http://www.springerlink.com/openurl.asp?genre=article&issn=1023-3830&volume=51&issue=4&spage=182
date added to LUP
2007-07-30 13:39:48
date last changed
2016-04-15 19:51:49
@article{6f0184d5-9a53-4ec5-b40a-29be3aeedaf3,
  abstract     = {OBJECTIVE: The purpose of this study was to examine the impact and mechanism of action of low molecular weight heparin (LMWH) in a model of murine colitis. MATERIALS AND METHODS: Balb/c mice were exposed to 5% dextran sodium sulfate (DSS) in the drinking water for five days. LMWH (500 units/kg/day) was administered by subcutaneous injection prior to and throughout the treatment period with DSS. Clinical disease activity index (DAI), including body weight loss, stool consistency and blood in feces were examined daily. Moreover, crypt height (CH), mucosal damage score (MDS), myeloperoxidase (MPO) activity and tumor necrosis factor-alpha (TNF-alpha) content in the colon were determined. RESULTS: DSS increased DAI, MDS, MPO activity and TNF-alpha production and decreased CH. Administration of LMWH markedly reduced DAI, MDS and reversed the CH-reduction. Moreover, in LMWH-treated animals, the MPO activity was reduced by more than 67% whereas mucosal levels of TNF-alpha was similar compared to DSS control mice. CONCLUSIONS: These findings suggest that LMWH inhibits murine colitis by interference with neutrophil recruitment and that LMWH may provide a novel pharmacological approach to treatment of inflammatory bowel disease.},
  author       = {Wan, M X and Liu, Qing and Wang, Yusheng and Thorlacius, Henrik},
  issn         = {1420-908X},
  keyword      = {Colitis,Leukocyte,Low molecular weight heparin,TNF-α},
  language     = {eng},
  number       = {4},
  pages        = {182--187},
  publisher    = {Birkhaüser},
  series       = {Inflammation Research1995-01-01+01:00},
  title        = {Protective effect of low molecular weight heparin on experimental colitis: role of neutrophil recruitment and TNF-alpha production.},
  volume       = {51},
  year         = {2002},
}