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The expression of retinoic Acid receptors and the effects in vitro by retinoids in human pancreatic cancer cell lines.

Albrechtsson, Elin; Ohlsson, Bodil and Axelson, Jan LU (2002) In Pancreas 25(1). p.49-49
Abstract
INTRODUCTION: Analogues of vitamin A have been shown to influence growth of malignant tissue, such as pancreatic cancer. AIMS: To study the expression of retinoic acid receptors (RAR) in pancreatic cancer cells and the effect of three different retinoids on the cell number in vitro were studied. METHODOLOGY: Cell lines were established from 13 patients who underwent surgery for pancreatic adenocarcinoma. The expression of the retinoic acid receptors (RAR) and retinoic X receptor (RXR) subtypes (alpha, beta, and gamma) was studied with western blotting and specific antibodies. The effect of incubation with all-trans-retinoic acid (atRA; tretinoin), 9-cis-retinoic acid (9-cis-RA), and 13-cis-retinoic acid (13-cis-RA; isotretinoin) on the... (More)
INTRODUCTION: Analogues of vitamin A have been shown to influence growth of malignant tissue, such as pancreatic cancer. AIMS: To study the expression of retinoic acid receptors (RAR) in pancreatic cancer cells and the effect of three different retinoids on the cell number in vitro were studied. METHODOLOGY: Cell lines were established from 13 patients who underwent surgery for pancreatic adenocarcinoma. The expression of the retinoic acid receptors (RAR) and retinoic X receptor (RXR) subtypes (alpha, beta, and gamma) was studied with western blotting and specific antibodies. The effect of incubation with all-trans-retinoic acid (atRA; tretinoin), 9-cis-retinoic acid (9-cis-RA), and 13-cis-retinoic acid (13-cis-RA; isotretinoin) on the cell number was examined with use of a Roche XTT cell proliferation kit. RESULTS: The RXRalpha receptor was expressed in all cell lines. RARalpha,beta and RXRbeta were expressed in most of them. RXRgamma was expressed in about half of the cell lines and RARgamma in only one. Incubation of the cells with retinoids showed a decreased cell number at concentrations of 104 M, except for 9-cis-RA, to which only about half of the cell lines responded. CONCLUSION: Two or more of the RAR subtypes were expressed in each pancreatic cell line. There was no uniform pattern of receptor expression; however, the cell lines responded with decreased cell number to high concentrations of atRA and 13-cis-RA but not to 9-cis-RA. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Pancreas
volume
25
issue
1
pages
49 - 49
publisher
Lippincott Williams & Wilkins
external identifiers
  • wos:000176591700009
  • pmid:12131771
  • scopus:0035990286
ISSN
0885-3177
language
English
LU publication?
yes
id
525fd945-aea8-44d1-b8d3-ae9ffb92232c (old id 109453)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12131771&dopt=Abstract
date added to LUP
2007-07-20 09:20:12
date last changed
2017-05-21 03:34:23
@article{525fd945-aea8-44d1-b8d3-ae9ffb92232c,
  abstract     = {INTRODUCTION: Analogues of vitamin A have been shown to influence growth of malignant tissue, such as pancreatic cancer. AIMS: To study the expression of retinoic acid receptors (RAR) in pancreatic cancer cells and the effect of three different retinoids on the cell number in vitro were studied. METHODOLOGY: Cell lines were established from 13 patients who underwent surgery for pancreatic adenocarcinoma. The expression of the retinoic acid receptors (RAR) and retinoic X receptor (RXR) subtypes (alpha, beta, and gamma) was studied with western blotting and specific antibodies. The effect of incubation with all-trans-retinoic acid (atRA; tretinoin), 9-cis-retinoic acid (9-cis-RA), and 13-cis-retinoic acid (13-cis-RA; isotretinoin) on the cell number was examined with use of a Roche XTT cell proliferation kit. RESULTS: The RXRalpha receptor was expressed in all cell lines. RARalpha,beta and RXRbeta were expressed in most of them. RXRgamma was expressed in about half of the cell lines and RARgamma in only one. Incubation of the cells with retinoids showed a decreased cell number at concentrations of 104 M, except for 9-cis-RA, to which only about half of the cell lines responded. CONCLUSION: Two or more of the RAR subtypes were expressed in each pancreatic cell line. There was no uniform pattern of receptor expression; however, the cell lines responded with decreased cell number to high concentrations of atRA and 13-cis-RA but not to 9-cis-RA.},
  author       = {Albrechtsson, Elin and Ohlsson, Bodil and Axelson, Jan},
  issn         = {0885-3177},
  language     = {eng},
  number       = {1},
  pages        = {49--49},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Pancreas},
  title        = {The expression of retinoic Acid receptors and the effects in vitro by retinoids in human pancreatic cancer cell lines.},
  volume       = {25},
  year         = {2002},
}