Advanced

Size and complexity of the nuclear genome of the ectomycorrhizal fungus Paxillus involutus.

Le Quéré, Antoine LU ; Johansson, Tomas LU and Tunlid, Anders LU (2002) In Fungal Genetics and Biology 36(3). p.234-241
Abstract
The basidiomycete Paxillus involutus is forming ectomycorrhizal symbiosis with a broad range of forest trees. Reassociation kinetics on P. involutus nuclear DNA indicated a haploid genome size of 23Mb including 11% of repetitive DNA. A similar genome size (20Mb) was estimated by genomic reconstruction analysis using three single copy genes. To assess the gene density in the P. involutus genome, a cosmid containing a 33-kb fragment of genomic DNA was sequenced and used to identify putative open reading frames (ORFs). Twelve potential ORFs were predicted, eight displayed significant sequence similarities to known proteins found in other organisms and notably, several homologues to the Podospora anserina vegetative incompatibility protein... (More)
The basidiomycete Paxillus involutus is forming ectomycorrhizal symbiosis with a broad range of forest trees. Reassociation kinetics on P. involutus nuclear DNA indicated a haploid genome size of 23Mb including 11% of repetitive DNA. A similar genome size (20Mb) was estimated by genomic reconstruction analysis using three single copy genes. To assess the gene density in the P. involutus genome, a cosmid containing a 33-kb fragment of genomic DNA was sequenced and used to identify putative open reading frames (ORFs). Twelve potential ORFs were predicted, eight displayed significant sequence similarities to known proteins found in other organisms and notably, several homologues to the Podospora anserina vegetative incompatibility protein (HetE1) were found. By extrapolation, we estimate the total number of genes in the P. involutus haploid genome to approximately 7700. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Fungal Genetics and Biology
volume
36
issue
3
pages
234 - 241
publisher
Elsevier
external identifiers
  • wos:000177376000008
  • scopus:0036349099
ISSN
1087-1845
DOI
10.1016/S1087-1845(02)00024-5
language
English
LU publication?
yes
id
74f8af45-9d86-409e-9b77-0ef76723d487 (old id 109580)
date added to LUP
2007-06-29 12:15:46
date last changed
2017-10-29 04:12:54
@article{74f8af45-9d86-409e-9b77-0ef76723d487,
  abstract     = {The basidiomycete Paxillus involutus is forming ectomycorrhizal symbiosis with a broad range of forest trees. Reassociation kinetics on P. involutus nuclear DNA indicated a haploid genome size of 23Mb including 11% of repetitive DNA. A similar genome size (20Mb) was estimated by genomic reconstruction analysis using three single copy genes. To assess the gene density in the P. involutus genome, a cosmid containing a 33-kb fragment of genomic DNA was sequenced and used to identify putative open reading frames (ORFs). Twelve potential ORFs were predicted, eight displayed significant sequence similarities to known proteins found in other organisms and notably, several homologues to the Podospora anserina vegetative incompatibility protein (HetE1) were found. By extrapolation, we estimate the total number of genes in the P. involutus haploid genome to approximately 7700.},
  author       = {Le Quéré, Antoine and Johansson, Tomas and Tunlid, Anders},
  issn         = {1087-1845},
  language     = {eng},
  number       = {3},
  pages        = {234--241},
  publisher    = {Elsevier},
  series       = {Fungal Genetics and Biology},
  title        = {Size and complexity of the nuclear genome of the ectomycorrhizal fungus Paxillus involutus.},
  url          = {http://dx.doi.org/10.1016/S1087-1845(02)00024-5},
  volume       = {36},
  year         = {2002},
}