The glucose/xylose facilitator Gxf1 from Candida intermedia expressed in a xylose-fermenting industrial strain of Saccharomyces cerevisiae increases xylose uptake in SSCF of wheat straw
(2011) In Enzyme and Microbial Technology 48(6-7). p.518-525- Abstract
- Ethanolic fermentation of lignocellulose raw materials requires industrial xylose-fermenting strains capable of complete and efficient D-xylose consumption. A central question in xylose fermentation by Saccharomyces cerevisiae engineered for xylose fermentation is to improve the xylose uptake. In the current study, the glucose/xylose facilitator Gxf1 from Candida intermedia, was expressed in three different xylose-fermenting S. cerevisiae strains of industrial origin. The in vivo effect on aerobic xylose growth and the initial xylose uptake rate were assessed. The expression of Gxf1 resulted in enhanced aerobic xylose growth only for the TMB3400 based strain. It displayed more than a 2-fold higher affinity for D-xylose than the parental... (More)
- Ethanolic fermentation of lignocellulose raw materials requires industrial xylose-fermenting strains capable of complete and efficient D-xylose consumption. A central question in xylose fermentation by Saccharomyces cerevisiae engineered for xylose fermentation is to improve the xylose uptake. In the current study, the glucose/xylose facilitator Gxf1 from Candida intermedia, was expressed in three different xylose-fermenting S. cerevisiae strains of industrial origin. The in vivo effect on aerobic xylose growth and the initial xylose uptake rate were assessed. The expression of Gxf1 resulted in enhanced aerobic xylose growth only for the TMB3400 based strain. It displayed more than a 2-fold higher affinity for D-xylose than the parental strain and approximately 2-fold higher initial specific growth rate at 4 g/L D-xylose. Enhanced xylose consumption was furthermore observed when the GXF1-strain was assessed in simultaneous saccharification and co-fermentation (SSCF) of pretreated wheat straw. However, the ethanol yield remained unchanged due to increased by-product formation. Metabolic flux analysis suggested that the expression of the Gxf1 transporter had shifted the control of xylose catabolism from transport to the NAD(+) dependent oxidation of xylitol to xylulose. (C) 2011 Elsevier Inc. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1985511
- author
- Fonseca, Cesar ; Olofsson, Kim LU ; Ferreira, Carla ; Runquist, David LU ; Fonseca, Luis L. ; Hahn-Hägerdal, Bärbel LU and Lidén, Gunnar LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Xylose transport, Industrial Saccharomyces cerevisiae, Simultaneous, saccharification and co-fermentation, Bioethanol, Gxf1
- in
- Enzyme and Microbial Technology
- volume
- 48
- issue
- 6-7
- pages
- 518 - 525
- publisher
- Elsevier
- external identifiers
-
- wos:000291283500013
- scopus:79955521875
- pmid:22113025
- ISSN
- 0141-0229
- DOI
- 10.1016/j.enzmictec.2011.02.010
- language
- English
- LU publication?
- yes
- id
- 1096bba5-fe39-4811-b81b-ba6769580b39 (old id 1985511)
- date added to LUP
- 2016-04-01 09:59:44
- date last changed
- 2025-04-04 14:29:08
@article{1096bba5-fe39-4811-b81b-ba6769580b39,
abstract = {{Ethanolic fermentation of lignocellulose raw materials requires industrial xylose-fermenting strains capable of complete and efficient D-xylose consumption. A central question in xylose fermentation by Saccharomyces cerevisiae engineered for xylose fermentation is to improve the xylose uptake. In the current study, the glucose/xylose facilitator Gxf1 from Candida intermedia, was expressed in three different xylose-fermenting S. cerevisiae strains of industrial origin. The in vivo effect on aerobic xylose growth and the initial xylose uptake rate were assessed. The expression of Gxf1 resulted in enhanced aerobic xylose growth only for the TMB3400 based strain. It displayed more than a 2-fold higher affinity for D-xylose than the parental strain and approximately 2-fold higher initial specific growth rate at 4 g/L D-xylose. Enhanced xylose consumption was furthermore observed when the GXF1-strain was assessed in simultaneous saccharification and co-fermentation (SSCF) of pretreated wheat straw. However, the ethanol yield remained unchanged due to increased by-product formation. Metabolic flux analysis suggested that the expression of the Gxf1 transporter had shifted the control of xylose catabolism from transport to the NAD(+) dependent oxidation of xylitol to xylulose. (C) 2011 Elsevier Inc. All rights reserved.}},
author = {{Fonseca, Cesar and Olofsson, Kim and Ferreira, Carla and Runquist, David and Fonseca, Luis L. and Hahn-Hägerdal, Bärbel and Lidén, Gunnar}},
issn = {{0141-0229}},
keywords = {{Xylose transport; Industrial Saccharomyces cerevisiae; Simultaneous; saccharification and co-fermentation; Bioethanol; Gxf1}},
language = {{eng}},
number = {{6-7}},
pages = {{518--525}},
publisher = {{Elsevier}},
series = {{Enzyme and Microbial Technology}},
title = {{The glucose/xylose facilitator Gxf1 from Candida intermedia expressed in a xylose-fermenting industrial strain of Saccharomyces cerevisiae increases xylose uptake in SSCF of wheat straw}},
url = {{http://dx.doi.org/10.1016/j.enzmictec.2011.02.010}},
doi = {{10.1016/j.enzmictec.2011.02.010}},
volume = {{48}},
year = {{2011}},
}