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Extracorporeal immunoadsorption from whole blood based on the avidin-biotin concept. Evaluation of a new method

Garkavij, Michael LU ; Tennvall, Jan LU ; Strand, Sven-Erik LU ; Nilsson, Rune LU ; Lindgren, Lars ; Chen, Jangin ; Isaksson, Martin ; Eriksson, Håkan and Sjögren, Hans-Olof (1996) In Acta Oncologica 35(3). p.309-312
Abstract
This study of 36 rats with rat colon adenocarcinoma transplants was carried out to investigate the efficacy of a new method of whole blood immunoadsorption (WBIA) in removing biotinylated monoclonal antibodies (MAbs) directly from unseparated blood, in order to increase 'the tumor/normal-tissue uptake ratio', as compared with extracorporeal immunoadsorption (ECIA) of antibodies from plasma. Compared with the ECIA system, the overall volume of the WBIA system (comprising only a pump, an adsorption column, a drop-chamber and tubings) was less (3.6 vs. 6.2 ml), and procedure duration 2 h less. The 17 rats undergoing the WBIA procedure, started 12 h after i.v. injection of 4.0-4.5 MBq 125I-BR96-biotin, manifested neither hemolysis nor any... (More)
This study of 36 rats with rat colon adenocarcinoma transplants was carried out to investigate the efficacy of a new method of whole blood immunoadsorption (WBIA) in removing biotinylated monoclonal antibodies (MAbs) directly from unseparated blood, in order to increase 'the tumor/normal-tissue uptake ratio', as compared with extracorporeal immunoadsorption (ECIA) of antibodies from plasma. Compared with the ECIA system, the overall volume of the WBIA system (comprising only a pump, an adsorption column, a drop-chamber and tubings) was less (3.6 vs. 6.2 ml), and procedure duration 2 h less. The 17 rats undergoing the WBIA procedure, started 12 h after i.v. injection of 4.0-4.5 MBq 125I-BR96-biotin, manifested neither hemolysis nor any other complication; no signs of organ edema were found at dissection; whole body and blood radioactivity values were reduced by 51% and 89.5%, respectively. The WBIA method was as effective as ECIA, but technically simpler, safer and more reliable. (Less)
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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Oncologica
volume
35
issue
3
pages
309 - 312
publisher
Taylor & Francis
external identifiers
  • pmid:8679261
  • scopus:0029894616
ISSN
1651-226X
DOI
10.3109/02841869609101646
language
English
LU publication?
yes
id
10c55935-bd9c-4c15-a855-ac5c41dcf476 (old id 1110285)
date added to LUP
2016-04-01 16:04:36
date last changed
2025-10-14 10:37:27
@article{10c55935-bd9c-4c15-a855-ac5c41dcf476,
  abstract     = {{This study of 36 rats with rat colon adenocarcinoma transplants was carried out to investigate the efficacy of a new method of whole blood immunoadsorption (WBIA) in removing biotinylated monoclonal antibodies (MAbs) directly from unseparated blood, in order to increase 'the tumor/normal-tissue uptake ratio', as compared with extracorporeal immunoadsorption (ECIA) of antibodies from plasma. Compared with the ECIA system, the overall volume of the WBIA system (comprising only a pump, an adsorption column, a drop-chamber and tubings) was less (3.6 vs. 6.2 ml), and procedure duration 2 h less. The 17 rats undergoing the WBIA procedure, started 12 h after i.v. injection of 4.0-4.5 MBq 125I-BR96-biotin, manifested neither hemolysis nor any other complication; no signs of organ edema were found at dissection; whole body and blood radioactivity values were reduced by 51% and 89.5%, respectively. The WBIA method was as effective as ECIA, but technically simpler, safer and more reliable.}},
  author       = {{Garkavij, Michael and Tennvall, Jan and Strand, Sven-Erik and Nilsson, Rune and Lindgren, Lars and Chen, Jangin and Isaksson, Martin and Eriksson, Håkan and Sjögren, Hans-Olof}},
  issn         = {{1651-226X}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{309--312}},
  publisher    = {{Taylor & Francis}},
  series       = {{Acta Oncologica}},
  title        = {{Extracorporeal immunoadsorption from whole blood based on the avidin-biotin concept. Evaluation of a new method}},
  url          = {{http://dx.doi.org/10.3109/02841869609101646}},
  doi          = {{10.3109/02841869609101646}},
  volume       = {{35}},
  year         = {{1996}},
}