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Bone-resorptive effects of endotoxin-contaminated high-density polyethylene particles spontaneously eliminated in vivo.

Skoglund, B; Larsson, L and Aspenberg, Per LU (2002) In Journal of Bone and Joint Surgery: British Volume 84(5). p.767-773
Abstract
Wear particles commonly used for experiments may carry adherent endotoxin on their surfaces, which may be responsible for the observed effects. In this study, we attached titanium plates to the tibiae of 20 rats. After osseointegration, endotoxin-contaminated or uncontaminated high-density-polyethylene (HDPE) particles were applied. Contaminated specimens showed a dramatic resorption of bone after seven days but new bone filled the site again at 21 days. Uncontaminated specimens showed no resorption. In 18 rats we implanted intramuscularly discs of ultra-high-molecular-weight polyethylene (UHMWPE) with baseline or excess contamination of endotoxin. Excess endotoxin disappeared within 24 hours and the amount of endotoxin remained at... (More)
Wear particles commonly used for experiments may carry adherent endotoxin on their surfaces, which may be responsible for the observed effects. In this study, we attached titanium plates to the tibiae of 20 rats. After osseointegration, endotoxin-contaminated or uncontaminated high-density-polyethylene (HDPE) particles were applied. Contaminated specimens showed a dramatic resorption of bone after seven days but new bone filled the site again at 21 days. Uncontaminated specimens showed no resorption. In 18 rats we implanted intramuscularly discs of ultra-high-molecular-weight polyethylene (UHMWPE) with baseline or excess contamination of endotoxin. Excess endotoxin disappeared within 24 hours and the amount of endotoxin remained at baseline level (contamination from production). Uncontaminated titanium discs did not adsorb endotoxin in vivo. The endotoxin was measured by analytical chemistry. Locally-applied endotoxin stimulated bone resorption similarly to that in experiments with wear particles. Endotoxin on the surface of implants and particles appeared to be inactivated in situ. A clean implant surface did not adsorb endotoxin. Our results suggest that endotoxin adhering to orthopaedic implants is not a major cause for concern. (Less)
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Contribution to journal
publication status
published
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in
Journal of Bone and Joint Surgery: British Volume
volume
84
issue
5
pages
767 - 773
publisher
British Editorial Society of Bone & Joint Surgery
external identifiers
  • wos:000177083100029
  • pmid:12188502
  • scopus:0036327446
ISSN
2044-5377
DOI
10.1302/0301-620X.84B5.11775
language
English
LU publication?
yes
id
67ec88f2-a99c-42b5-a040-46440b8f01a3 (old id 110025)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12188502&dopt=Abstract
date added to LUP
2007-07-04 09:25:55
date last changed
2017-12-05 10:31:36
@article{67ec88f2-a99c-42b5-a040-46440b8f01a3,
  abstract     = {Wear particles commonly used for experiments may carry adherent endotoxin on their surfaces, which may be responsible for the observed effects. In this study, we attached titanium plates to the tibiae of 20 rats. After osseointegration, endotoxin-contaminated or uncontaminated high-density-polyethylene (HDPE) particles were applied. Contaminated specimens showed a dramatic resorption of bone after seven days but new bone filled the site again at 21 days. Uncontaminated specimens showed no resorption. In 18 rats we implanted intramuscularly discs of ultra-high-molecular-weight polyethylene (UHMWPE) with baseline or excess contamination of endotoxin. Excess endotoxin disappeared within 24 hours and the amount of endotoxin remained at baseline level (contamination from production). Uncontaminated titanium discs did not adsorb endotoxin in vivo. The endotoxin was measured by analytical chemistry. Locally-applied endotoxin stimulated bone resorption similarly to that in experiments with wear particles. Endotoxin on the surface of implants and particles appeared to be inactivated in situ. A clean implant surface did not adsorb endotoxin. Our results suggest that endotoxin adhering to orthopaedic implants is not a major cause for concern.},
  author       = {Skoglund, B and Larsson, L and Aspenberg, Per},
  issn         = {2044-5377},
  language     = {eng},
  number       = {5},
  pages        = {767--773},
  publisher    = {British Editorial Society of Bone & Joint Surgery},
  series       = {Journal of Bone and Joint Surgery: British Volume},
  title        = {Bone-resorptive effects of endotoxin-contaminated high-density polyethylene particles spontaneously eliminated in vivo.},
  url          = {http://dx.doi.org/10.1302/0301-620X.84B5.11775},
  volume       = {84},
  year         = {2002},
}