Bone-resorptive effects of endotoxin-contaminated high-density polyethylene particles spontaneously eliminated in vivo.
(2002) In Journal of Bone and Joint Surgery: British Volume 84(5). p.767-773- Abstract
- Wear particles commonly used for experiments may carry adherent endotoxin on their surfaces, which may be responsible for the observed effects. In this study, we attached titanium plates to the tibiae of 20 rats. After osseointegration, endotoxin-contaminated or uncontaminated high-density-polyethylene (HDPE) particles were applied. Contaminated specimens showed a dramatic resorption of bone after seven days but new bone filled the site again at 21 days. Uncontaminated specimens showed no resorption. In 18 rats we implanted intramuscularly discs of ultra-high-molecular-weight polyethylene (UHMWPE) with baseline or excess contamination of endotoxin. Excess endotoxin disappeared within 24 hours and the amount of endotoxin remained at... (More)
- Wear particles commonly used for experiments may carry adherent endotoxin on their surfaces, which may be responsible for the observed effects. In this study, we attached titanium plates to the tibiae of 20 rats. After osseointegration, endotoxin-contaminated or uncontaminated high-density-polyethylene (HDPE) particles were applied. Contaminated specimens showed a dramatic resorption of bone after seven days but new bone filled the site again at 21 days. Uncontaminated specimens showed no resorption. In 18 rats we implanted intramuscularly discs of ultra-high-molecular-weight polyethylene (UHMWPE) with baseline or excess contamination of endotoxin. Excess endotoxin disappeared within 24 hours and the amount of endotoxin remained at baseline level (contamination from production). Uncontaminated titanium discs did not adsorb endotoxin in vivo. The endotoxin was measured by analytical chemistry. Locally-applied endotoxin stimulated bone resorption similarly to that in experiments with wear particles. Endotoxin on the surface of implants and particles appeared to be inactivated in situ. A clean implant surface did not adsorb endotoxin. Our results suggest that endotoxin adhering to orthopaedic implants is not a major cause for concern. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/110025
- author
- Skoglund, B ; Larsson, L and Aspenberg, Per LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Bone and Joint Surgery: British Volume
- volume
- 84
- issue
- 5
- pages
- 767 - 773
- publisher
- British Editorial Society of Bone & Joint Surgery
- external identifiers
-
- wos:000177083100029
- pmid:12188502
- scopus:0036327446
- ISSN
- 2044-5377
- DOI
- 10.1302/0301-620X.84B5.11775
- language
- English
- LU publication?
- yes
- id
- 67ec88f2-a99c-42b5-a040-46440b8f01a3 (old id 110025)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12188502&dopt=Abstract
- date added to LUP
- 2016-04-01 11:43:51
- date last changed
- 2022-04-28 19:11:39
@article{67ec88f2-a99c-42b5-a040-46440b8f01a3,
abstract = {{Wear particles commonly used for experiments may carry adherent endotoxin on their surfaces, which may be responsible for the observed effects. In this study, we attached titanium plates to the tibiae of 20 rats. After osseointegration, endotoxin-contaminated or uncontaminated high-density-polyethylene (HDPE) particles were applied. Contaminated specimens showed a dramatic resorption of bone after seven days but new bone filled the site again at 21 days. Uncontaminated specimens showed no resorption. In 18 rats we implanted intramuscularly discs of ultra-high-molecular-weight polyethylene (UHMWPE) with baseline or excess contamination of endotoxin. Excess endotoxin disappeared within 24 hours and the amount of endotoxin remained at baseline level (contamination from production). Uncontaminated titanium discs did not adsorb endotoxin in vivo. The endotoxin was measured by analytical chemistry. Locally-applied endotoxin stimulated bone resorption similarly to that in experiments with wear particles. Endotoxin on the surface of implants and particles appeared to be inactivated in situ. A clean implant surface did not adsorb endotoxin. Our results suggest that endotoxin adhering to orthopaedic implants is not a major cause for concern.}},
author = {{Skoglund, B and Larsson, L and Aspenberg, Per}},
issn = {{2044-5377}},
language = {{eng}},
number = {{5}},
pages = {{767--773}},
publisher = {{British Editorial Society of Bone & Joint Surgery}},
series = {{Journal of Bone and Joint Surgery: British Volume}},
title = {{Bone-resorptive effects of endotoxin-contaminated high-density polyethylene particles spontaneously eliminated in vivo.}},
url = {{http://dx.doi.org/10.1302/0301-620X.84B5.11775}},
doi = {{10.1302/0301-620X.84B5.11775}},
volume = {{84}},
year = {{2002}},
}