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Joint inflammation in rabbits induced by preformed immune complexes

Bjursten, Lars Magnus LU ; Bagge, U and Ahlstedt, S (1981) In International Archives of Allergy and Applied Immunology 64(1). p.67-71
Abstract
The rabbit knee joint was used to evaluate the inflammatory properties of immune complexes with different contents of antigen. Immune complexes made in vitro with antiserum from hyperimmunized rabbits and bovine serum albumin as antigen were injected into the joints. The reactivity of the individual animal was established by injection of normal rabbit serum into the contralateral joint. The number of leukocytes washed out from the joints at fixed intervals was used as a measure of the inflammatory properties of the immune complexes. With immune complexes formed at antigen excess the highest numbers of leukocytes, with a predominance of granulocytes, were found after 6 h of exposure. However, these complexes gave only weak complement... (More)
The rabbit knee joint was used to evaluate the inflammatory properties of immune complexes with different contents of antigen. Immune complexes made in vitro with antiserum from hyperimmunized rabbits and bovine serum albumin as antigen were injected into the joints. The reactivity of the individual animal was established by injection of normal rabbit serum into the contralateral joint. The number of leukocytes washed out from the joints at fixed intervals was used as a measure of the inflammatory properties of the immune complexes. With immune complexes formed at antigen excess the highest numbers of leukocytes, with a predominance of granulocytes, were found after 6 h of exposure. However, these complexes gave only weak complement activation in vitro, measured with a hemolysis assay. Complexes formed at optimal precipitation proportions gave high complement activation in vitro but only a small number of leukocytes in the joints at 6 h exposure. This finding may be explained by a rapid phagocytosis of the latter complexes while complexes formed at antigen excess are not readily phagocytized. This hypothesis is supported by the observation that higher leukocyte counts were found after shorter exposure times to complexes formed at optimal precipitation proportions than after exposure to complexes with antigen excess. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Archives of Allergy and Applied Immunology
volume
64
issue
1
pages
67 - 71
publisher
Karger
external identifiers
  • pmid:7450903
  • scopus:0019365757
ISSN
0020-5915
language
English
LU publication?
yes
id
de7dee76-73d1-46c8-94f5-438962a6f0c6 (old id 1102807)
date added to LUP
2008-08-13 15:22:20
date last changed
2017-08-27 05:40:24
@article{de7dee76-73d1-46c8-94f5-438962a6f0c6,
  abstract     = {The rabbit knee joint was used to evaluate the inflammatory properties of immune complexes with different contents of antigen. Immune complexes made in vitro with antiserum from hyperimmunized rabbits and bovine serum albumin as antigen were injected into the joints. The reactivity of the individual animal was established by injection of normal rabbit serum into the contralateral joint. The number of leukocytes washed out from the joints at fixed intervals was used as a measure of the inflammatory properties of the immune complexes. With immune complexes formed at antigen excess the highest numbers of leukocytes, with a predominance of granulocytes, were found after 6 h of exposure. However, these complexes gave only weak complement activation in vitro, measured with a hemolysis assay. Complexes formed at optimal precipitation proportions gave high complement activation in vitro but only a small number of leukocytes in the joints at 6 h exposure. This finding may be explained by a rapid phagocytosis of the latter complexes while complexes formed at antigen excess are not readily phagocytized. This hypothesis is supported by the observation that higher leukocyte counts were found after shorter exposure times to complexes formed at optimal precipitation proportions than after exposure to complexes with antigen excess.},
  author       = {Bjursten, Lars Magnus and Bagge, U and Ahlstedt, S},
  issn         = {0020-5915},
  language     = {eng},
  number       = {1},
  pages        = {67--71},
  publisher    = {Karger},
  series       = {International Archives of Allergy and Applied Immunology},
  title        = {Joint inflammation in rabbits induced by preformed immune complexes},
  volume       = {64},
  year         = {1981},
}