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IgM antibody to the hepatitis B core antigen in acute hepatitis determined by SPRIA--diagnostic value

Widell, Anders LU ; Hansson, B G; Lofgren, B; Moestrup, T; Norkrans, G; Johnsson, T and Nordenfelt, E (1982) In Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology 90(1). p.79-84
Abstract
A solid phase radio-immunoassay (SPRIA) was developed for the detection of anti-HBc IgM. The assay proved sensitive and easy to perform and rheumatoid factor did not affect the test results. Anti-HBc IgM titres were followed in consecutive samples from 15 patients after uncomplicated acute hepatitis B. In the acute phase anti-HBc IgM titres ranged from 10(-5) to 10(-7) (mean 10(-6.4)). One year after onset of disease ten of the 15 had titres below 10(-4) and between two and three years after onset most patients had titres 10(-3). Anti-HBc IgM titres were determined in six episodes of acute hepatitis B, all HBsAg negative but anti-HBc positive in the first samples obtained (within 8 days) and developing anti-HBs during convalescence. Acute... (More)
A solid phase radio-immunoassay (SPRIA) was developed for the detection of anti-HBc IgM. The assay proved sensitive and easy to perform and rheumatoid factor did not affect the test results. Anti-HBc IgM titres were followed in consecutive samples from 15 patients after uncomplicated acute hepatitis B. In the acute phase anti-HBc IgM titres ranged from 10(-5) to 10(-7) (mean 10(-6.4)). One year after onset of disease ten of the 15 had titres below 10(-4) and between two and three years after onset most patients had titres 10(-3). Anti-HBc IgM titres were determined in six episodes of acute hepatitis B, all HBsAg negative but anti-HBc positive in the first samples obtained (within 8 days) and developing anti-HBs during convalescence. Acute phase anti-HBc IgM titres in these patients ranged between 10(-5.5) and 10(-7) (mean 10(-6.5)) and were thus identical with HBsAg positive cases. When acute phase sera from 168 episodes of acute hepatitis primarily classified as non-A, non-B, were tested for anti-HBc IgM titres above 10(-5), sera from 13 episodes were positive and in seven of these hepatitis B diagnosis could be confirmed by rising anti-HBs titres in convalescence. Sera from four of the 13 patients contained HBeAg, which was thus demonstrated in the absence of HBsAg. The results show that testing for anti-HBc IgM is important for a true non-A, non-B diagnosis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology
volume
90
issue
1
pages
79 - 84
publisher
Munksgaard International Publishers
external identifiers
  • pmid:7080827
  • scopus:0020063843
ISSN
0108-0180
language
English
LU publication?
yes
id
ba86e98e-6026-4731-b4b1-b5f0526953e9 (old id 1102904)
date added to LUP
2008-08-13 13:32:20
date last changed
2017-07-30 04:40:33
@article{ba86e98e-6026-4731-b4b1-b5f0526953e9,
  abstract     = {A solid phase radio-immunoassay (SPRIA) was developed for the detection of anti-HBc IgM. The assay proved sensitive and easy to perform and rheumatoid factor did not affect the test results. Anti-HBc IgM titres were followed in consecutive samples from 15 patients after uncomplicated acute hepatitis B. In the acute phase anti-HBc IgM titres ranged from 10(-5) to 10(-7) (mean 10(-6.4)). One year after onset of disease ten of the 15 had titres below 10(-4) and between two and three years after onset most patients had titres 10(-3). Anti-HBc IgM titres were determined in six episodes of acute hepatitis B, all HBsAg negative but anti-HBc positive in the first samples obtained (within 8 days) and developing anti-HBs during convalescence. Acute phase anti-HBc IgM titres in these patients ranged between 10(-5.5) and 10(-7) (mean 10(-6.5)) and were thus identical with HBsAg positive cases. When acute phase sera from 168 episodes of acute hepatitis primarily classified as non-A, non-B, were tested for anti-HBc IgM titres above 10(-5), sera from 13 episodes were positive and in seven of these hepatitis B diagnosis could be confirmed by rising anti-HBs titres in convalescence. Sera from four of the 13 patients contained HBeAg, which was thus demonstrated in the absence of HBsAg. The results show that testing for anti-HBc IgM is important for a true non-A, non-B diagnosis.},
  author       = {Widell, Anders and Hansson, B G and Lofgren, B and Moestrup, T and Norkrans, G and Johnsson, T and Nordenfelt, E},
  issn         = {0108-0180},
  language     = {eng},
  number       = {1},
  pages        = {79--84},
  publisher    = {Munksgaard International Publishers},
  series       = {Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology},
  title        = {IgM antibody to the hepatitis B core antigen in acute hepatitis determined by SPRIA--diagnostic value},
  volume       = {90},
  year         = {1982},
}