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The kinetics of leucocyte migration into rabbit knee joints elicited by preformed immune complexes with different in vitro characteristics

Bjursten, Lars Magnus LU ; Thomsen, P; Ahlstedt, S and Bagge, U (1983) In Immunology 49(2). p.205-213
Abstract
Immune complexes were formed in vitro with antibodies obtained from rabbits immunized with bovine serum albumin in Freund's complete (FCA) or incomplete (FIA) adjuvant. The antibodies were mixed with different amounts of antigen. Immune complexes formed at maximum precipitation proportions were efficient in complement activation, whereas immune complexes formed at antigen excess had weak complement-activating properties. When injected into rabbit knee joints, the immune complexes formed at maximum precipitation proportions with FCA or FIA antibodies caused a moderate leucocyte migration into the joints with maximum cell counts 6-8 hr after injection. Injection into the joints of immune complexes formed with FCA antibodies at antigen excess... (More)
Immune complexes were formed in vitro with antibodies obtained from rabbits immunized with bovine serum albumin in Freund's complete (FCA) or incomplete (FIA) adjuvant. The antibodies were mixed with different amounts of antigen. Immune complexes formed at maximum precipitation proportions were efficient in complement activation, whereas immune complexes formed at antigen excess had weak complement-activating properties. When injected into rabbit knee joints, the immune complexes formed at maximum precipitation proportions with FCA or FIA antibodies caused a moderate leucocyte migration into the joints with maximum cell counts 6-8 hr after injection. Injection into the joints of immune complexes formed with FCA antibodies at antigen excess induced a pronounced leucocyte migration with maximum numbers 18 hr after injection. Immunofluorescent studies indicated that these immune complexes became associated with the leucocyte membrane, whereas immune complexes formed at maximum precipitation proportions were found in the leucocyte granulae, indicating that only the complement-activating immune complexes were efficiently eliminated. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Immunology
volume
49
issue
2
pages
205 - 213
publisher
Wiley-Blackwell
external identifiers
  • pmid:6852865
  • scopus:0020537083
ISSN
0019-2805
language
English
LU publication?
yes
id
40043f84-8fd3-4cff-a495-1c36b7683a38 (old id 1103004)
alternative location
http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1454179&blobtype=pdf
date added to LUP
2008-08-13 11:12:09
date last changed
2017-01-01 04:22:55
@article{40043f84-8fd3-4cff-a495-1c36b7683a38,
  abstract     = {Immune complexes were formed in vitro with antibodies obtained from rabbits immunized with bovine serum albumin in Freund's complete (FCA) or incomplete (FIA) adjuvant. The antibodies were mixed with different amounts of antigen. Immune complexes formed at maximum precipitation proportions were efficient in complement activation, whereas immune complexes formed at antigen excess had weak complement-activating properties. When injected into rabbit knee joints, the immune complexes formed at maximum precipitation proportions with FCA or FIA antibodies caused a moderate leucocyte migration into the joints with maximum cell counts 6-8 hr after injection. Injection into the joints of immune complexes formed with FCA antibodies at antigen excess induced a pronounced leucocyte migration with maximum numbers 18 hr after injection. Immunofluorescent studies indicated that these immune complexes became associated with the leucocyte membrane, whereas immune complexes formed at maximum precipitation proportions were found in the leucocyte granulae, indicating that only the complement-activating immune complexes were efficiently eliminated.},
  author       = {Bjursten, Lars Magnus and Thomsen, P and Ahlstedt, S and Bagge, U},
  issn         = {0019-2805},
  language     = {eng},
  number       = {2},
  pages        = {205--213},
  publisher    = {Wiley-Blackwell},
  series       = {Immunology},
  title        = {The kinetics of leucocyte migration into rabbit knee joints elicited by preformed immune complexes with different in vitro characteristics},
  volume       = {49},
  year         = {1983},
}