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False positive results in class-specific rheumatoid factor (RF) assays due to interaction between RF and Fc fragments of anti-immunoglobulin indicator reagents

Tarkowski, Andrej; Bjursten, Lars Magnus LU ; Nilsson, Lars-Åke and Nygren, Håkan (1983) In Journal of Immunological Methods 58(1-2). p.171-182
Abstract
A recently developed, simple immunological method, diffusion-in-gel ELISA, has been adapted for class-specific determination of rheumatoid factor (RF). Evidence was obtained of an analytical error due to interaction between RF and antigenic determinants on the Fc part of the indicator immunoglobulin (Ig) molecules used. This was eliminated by replacing the usual indicator reagent, complete Ig molecules, by conjugated Fab or F(ab')2 fragments. The findings imply that unless the RF-Fc interaction mentioned is avoided in the assaying technique, false positive results may be obtained for, e.g. IgG RF and IgA RF in sera containing IgM RF.
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
rheumatoid factor, immunoassay, immunoglobulin class
in
Journal of Immunological Methods
volume
58
issue
1-2
pages
171 - 182
publisher
Elsevier
external identifiers
  • pmid:6601157
  • scopus:0020672078
ISSN
1872-7905
DOI
10.1016/0022-1759(83)90273-9
language
English
LU publication?
no
id
e24f121c-58c3-4fa9-bec5-73311b5f5f05 (old id 1103008)
date added to LUP
2008-08-13 11:14:32
date last changed
2017-01-01 07:04:38
@article{e24f121c-58c3-4fa9-bec5-73311b5f5f05,
  abstract     = {A recently developed, simple immunological method, diffusion-in-gel ELISA, has been adapted for class-specific determination of rheumatoid factor (RF). Evidence was obtained of an analytical error due to interaction between RF and antigenic determinants on the Fc part of the indicator immunoglobulin (Ig) molecules used. This was eliminated by replacing the usual indicator reagent, complete Ig molecules, by conjugated Fab or F(ab')2 fragments. The findings imply that unless the RF-Fc interaction mentioned is avoided in the assaying technique, false positive results may be obtained for, e.g. IgG RF and IgA RF in sera containing IgM RF.},
  author       = {Tarkowski, Andrej and Bjursten, Lars Magnus and Nilsson, Lars-Åke and Nygren, Håkan},
  issn         = {1872-7905},
  keyword      = {rheumatoid factor,immunoassay,immunoglobulin class},
  language     = {eng},
  number       = {1-2},
  pages        = {171--182},
  publisher    = {Elsevier},
  series       = {Journal of Immunological Methods},
  title        = {False positive results in class-specific rheumatoid factor (RF) assays due to interaction between RF and Fc fragments of anti-immunoglobulin indicator reagents},
  url          = {http://dx.doi.org/10.1016/0022-1759(83)90273-9},
  volume       = {58},
  year         = {1983},
}