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An experimental method for in vivo studies of pulmonary platelet sequestration

Thörne, Johan LU ; Olsson, Per-Ingvar; Strand, Sven-Erik LU ; Jönsson, Bo-Anders LU and Norgren, Lars LU (1984) In European Journal of Nuclear Medicine and Molecular Imaging 9(10). p.472-477
Abstract
It has been reported in earlier in vitro studies that soft tissue trauma and endotoxin-induced shock causes pulmonary platelet trapping (PPT). This paper describes a noninvasive in vivo technique for dynamic studies of PPT in rabbits. Autologous platelets were labeled with 111In and reinfused into the animals. The following day, the rabbits were anesthetized and placed in a supine position under a scintillation camera. Continuous measurement of the activity distribution in the animal was performed for 35 min. The first 5 min represented a preshock measurement, whereafter endotoxin E. coli was injected IV. The following 2-3 min showed a sudden increase of radioactivity in the lungs indicating PPT, and thereafter a slow decrease to almost... (More)
It has been reported in earlier in vitro studies that soft tissue trauma and endotoxin-induced shock causes pulmonary platelet trapping (PPT). This paper describes a noninvasive in vivo technique for dynamic studies of PPT in rabbits. Autologous platelets were labeled with 111In and reinfused into the animals. The following day, the rabbits were anesthetized and placed in a supine position under a scintillation camera. Continuous measurement of the activity distribution in the animal was performed for 35 min. The first 5 min represented a preshock measurement, whereafter endotoxin E. coli was injected IV. The following 2-3 min showed a sudden increase of radioactivity in the lungs indicating PPT, and thereafter a slow decrease to almost the preshock level. A simultaneous decrease in the number of platelets and the radioactivity in peripheral blood also indicated the induction of PPT. This study clearly shows that PPT can be detected in vivo with an easy, noninvasive scintillation camera method (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
European Journal of Nuclear Medicine and Molecular Imaging
volume
9
issue
10
pages
472 - 477
publisher
Springer
external identifiers
  • scopus:0021173330
ISSN
1619-7070
DOI
10.1007/BF00563172
language
English
LU publication?
yes
id
82c4c358-4b50-4ba2-a4ea-fe7d682b0902 (old id 1103340)
date added to LUP
2008-08-12 16:38:53
date last changed
2017-08-27 03:55:21
@article{82c4c358-4b50-4ba2-a4ea-fe7d682b0902,
  abstract     = {It has been reported in earlier in vitro studies that soft tissue trauma and endotoxin-induced shock causes pulmonary platelet trapping (PPT). This paper describes a noninvasive in vivo technique for dynamic studies of PPT in rabbits. Autologous platelets were labeled with 111In and reinfused into the animals. The following day, the rabbits were anesthetized and placed in a supine position under a scintillation camera. Continuous measurement of the activity distribution in the animal was performed for 35 min. The first 5 min represented a preshock measurement, whereafter endotoxin E. coli was injected IV. The following 2-3 min showed a sudden increase of radioactivity in the lungs indicating PPT, and thereafter a slow decrease to almost the preshock level. A simultaneous decrease in the number of platelets and the radioactivity in peripheral blood also indicated the induction of PPT. This study clearly shows that PPT can be detected in vivo with an easy, noninvasive scintillation camera method},
  author       = {Thörne, Johan and Olsson, Per-Ingvar and Strand, Sven-Erik and Jönsson, Bo-Anders and Norgren, Lars},
  issn         = {1619-7070},
  language     = {eng},
  number       = {10},
  pages        = {472--477},
  publisher    = {Springer},
  series       = {European Journal of Nuclear Medicine and Molecular Imaging},
  title        = {An experimental method for in vivo studies of pulmonary platelet sequestration},
  url          = {http://dx.doi.org/10.1007/BF00563172},
  volume       = {9},
  year         = {1984},
}