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Acute synovitis induced by preformed immune complexes

Thomsen, P; Bjursten, Lars Magnus LU ; Hansson, H A and Bagge, U (1986) In Scandinavian Journal of Rheumatology 15(2). p.134-142
Abstract
The morphology of acute immune complex (IC) elicited synovitis in rabbit knee joints was studied, as well as IC-induced leukocyte activation in vivo and in vitro. Acute synovitis was induced by intra-articular injection of in vitro preformed, complement activating bovine serum albumin (BSA)-anti-BSA IC. Within 30 min, migration of polymorphonuclear granulocytes (PMNGs) was observed. Scanning electron microscopy showed that adhering, apparently activated leukocytes were attached to the synovial lining, often forming clusters. Phagocytosis of IC was evident, as immunoglobulins were detected within the leukocyte cytoplasm by the direct immunofluorescence technique. At the peak accumulation of PMNGs, focal erosions of the synovial lining were... (More)
The morphology of acute immune complex (IC) elicited synovitis in rabbit knee joints was studied, as well as IC-induced leukocyte activation in vivo and in vitro. Acute synovitis was induced by intra-articular injection of in vitro preformed, complement activating bovine serum albumin (BSA)-anti-BSA IC. Within 30 min, migration of polymorphonuclear granulocytes (PMNGs) was observed. Scanning electron microscopy showed that adhering, apparently activated leukocytes were attached to the synovial lining, often forming clusters. Phagocytosis of IC was evident, as immunoglobulins were detected within the leukocyte cytoplasm by the direct immunofluorescence technique. At the peak accumulation of PMNGs, focal erosions of the synovial lining were observed. Later, monocytes and macrophages appeared and degenerated PMNGs were found, sometimes within the cytoplasm of large macrophages. Chemiluminescence experiments showed a maximum in vitro activation of leukocytes by complement activating IC formed near optimal precipitation proportions and in slight antigen excess, whereas IC in large antigen excess gave a smaller and later response. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Scandinavian Journal of Rheumatology
volume
15
issue
2
pages
134 - 142
publisher
Taylor & Francis
external identifiers
  • pmid:3749826
  • scopus:0022442409
ISSN
1502-7732
DOI
10.3109/03009748609102079
language
English
LU publication?
yes
id
1f7c4708-2e6b-47ab-b00c-b297fac40bb2 (old id 1103506)
date added to LUP
2008-08-11 08:43:30
date last changed
2017-01-01 04:35:38
@article{1f7c4708-2e6b-47ab-b00c-b297fac40bb2,
  abstract     = {The morphology of acute immune complex (IC) elicited synovitis in rabbit knee joints was studied, as well as IC-induced leukocyte activation in vivo and in vitro. Acute synovitis was induced by intra-articular injection of in vitro preformed, complement activating bovine serum albumin (BSA)-anti-BSA IC. Within 30 min, migration of polymorphonuclear granulocytes (PMNGs) was observed. Scanning electron microscopy showed that adhering, apparently activated leukocytes were attached to the synovial lining, often forming clusters. Phagocytosis of IC was evident, as immunoglobulins were detected within the leukocyte cytoplasm by the direct immunofluorescence technique. At the peak accumulation of PMNGs, focal erosions of the synovial lining were observed. Later, monocytes and macrophages appeared and degenerated PMNGs were found, sometimes within the cytoplasm of large macrophages. Chemiluminescence experiments showed a maximum in vitro activation of leukocytes by complement activating IC formed near optimal precipitation proportions and in slight antigen excess, whereas IC in large antigen excess gave a smaller and later response.},
  author       = {Thomsen, P and Bjursten, Lars Magnus and Hansson, H A and Bagge, U},
  issn         = {1502-7732},
  language     = {eng},
  number       = {2},
  pages        = {134--142},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Rheumatology},
  title        = {Acute synovitis induced by preformed immune complexes},
  url          = {http://dx.doi.org/10.3109/03009748609102079},
  volume       = {15},
  year         = {1986},
}