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The biochemistry of the action of chymopapain in relief of sciatica

Buttle, David; Abrahamson, Magnus LU and Barrett, Alan J (1986) In Spine 11(7). p.688-694
Abstract
A study has been made of the mechanism of action of intradiscal injections of preparations of chymopapain in the treatment of sciatica. Such preparations were found to contain at least four distinct proteins, but enzymatically active chymopapain was the component mainly responsible for releasing glycosaminoglycan from cartilaginous tissue. Previous suggestions that an electrostatic interaction between chymopapain and glycosaminoglycan is important to the action of injected enzyme were not supported by the finding that both positively and negatively charged forms of chymopapain efficiently released glycosaminoglycan from cartilaginous tissue. In contrast, cysteine alone did not cause release of glycosaminoglycan. Chymopapain was found to be... (More)
A study has been made of the mechanism of action of intradiscal injections of preparations of chymopapain in the treatment of sciatica. Such preparations were found to contain at least four distinct proteins, but enzymatically active chymopapain was the component mainly responsible for releasing glycosaminoglycan from cartilaginous tissue. Previous suggestions that an electrostatic interaction between chymopapain and glycosaminoglycan is important to the action of injected enzyme were not supported by the finding that both positively and negatively charged forms of chymopapain efficiently released glycosaminoglycan from cartilaginous tissue. In contrast, cysteine alone did not cause release of glycosaminoglycan. Chymopapain was found to be inhibited efficiently by the protein inhibitors, cystatin C and low molecular weight kininogen in vitro, and the possible relevance of this finding to the efficacy and safety of chemonucleolysis is discussed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Spine
volume
11
issue
7
pages
688 - 694
publisher
Lippincott Williams & Wilkins
external identifiers
  • scopus:0023022455
ISSN
0362-2436
language
English
LU publication?
yes
id
fec2b2d2-274d-4077-b48d-14abb5587ee4 (old id 1103704)
date added to LUP
2008-08-11 11:17:17
date last changed
2017-01-01 06:41:48
@article{fec2b2d2-274d-4077-b48d-14abb5587ee4,
  abstract     = {A study has been made of the mechanism of action of intradiscal injections of preparations of chymopapain in the treatment of sciatica. Such preparations were found to contain at least four distinct proteins, but enzymatically active chymopapain was the component mainly responsible for releasing glycosaminoglycan from cartilaginous tissue. Previous suggestions that an electrostatic interaction between chymopapain and glycosaminoglycan is important to the action of injected enzyme were not supported by the finding that both positively and negatively charged forms of chymopapain efficiently released glycosaminoglycan from cartilaginous tissue. In contrast, cysteine alone did not cause release of glycosaminoglycan. Chymopapain was found to be inhibited efficiently by the protein inhibitors, cystatin C and low molecular weight kininogen in vitro, and the possible relevance of this finding to the efficacy and safety of chemonucleolysis is discussed.},
  author       = {Buttle, David and Abrahamson, Magnus and Barrett, Alan J},
  issn         = {0362-2436},
  language     = {eng},
  number       = {7},
  pages        = {688--694},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Spine},
  title        = {The biochemistry of the action of chymopapain in relief of sciatica},
  volume       = {11},
  year         = {1986},
}