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Molecular cloning and sequence analysis of cDNA coding for the precursor of the human cysteine proteinase inhibitor cystatin C

Abrahamson, Magnus LU ; Grubb, Anders LU ; Olafsson, Isleifur and Lundwall, Åke LU (1987) In FEBS Letters 216(2). p.229-233
Abstract
Recombinant cystatin C producing clones were isolated from a human placenta λgt11 cDNA library. The cDNA insert of one of the clones, containing 777 base pairs, encodes the complete mature cystatin C (120 amino acids) and a hydrophobic leader sequence of 26 amino acids, indicating an extracellular function of the inhibitor. The deduced protein sequence confirms the protein sequence of cystatin C isolated from human urine, but differs in one position from the sequence of the cystatin C fragment deposited as amyloid in hereditary cerebral hemorrhage with amyloidosis.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Enzyme inhibitor, Cysteine proteinase, Cerebral hemorrhage, Cystatin C, cDNA cloning, Nucleotide sequence
in
FEBS Letters
volume
216
issue
2
pages
229 - 233
publisher
Wiley-Blackwell
external identifiers
  • scopus:0023229953
ISSN
1873-3468
DOI
10.1016/0014-5793(87)80695-6
language
English
LU publication?
yes
id
25e8f596-c541-477a-b554-15eed7bd0981 (old id 1104011)
date added to LUP
2008-08-08 17:43:35
date last changed
2017-08-06 04:42:36
@article{25e8f596-c541-477a-b554-15eed7bd0981,
  abstract     = {Recombinant cystatin C producing clones were isolated from a human placenta λgt11 cDNA library. The cDNA insert of one of the clones, containing 777 base pairs, encodes the complete mature cystatin C (120 amino acids) and a hydrophobic leader sequence of 26 amino acids, indicating an extracellular function of the inhibitor. The deduced protein sequence confirms the protein sequence of cystatin C isolated from human urine, but differs in one position from the sequence of the cystatin C fragment deposited as amyloid in hereditary cerebral hemorrhage with amyloidosis.},
  author       = {Abrahamson, Magnus and Grubb, Anders and Olafsson, Isleifur and Lundwall, Åke},
  issn         = {1873-3468},
  keyword      = {Enzyme inhibitor,Cysteine proteinase,Cerebral hemorrhage,Cystatin C,cDNA cloning,Nucleotide sequence},
  language     = {eng},
  number       = {2},
  pages        = {229--233},
  publisher    = {Wiley-Blackwell},
  series       = {FEBS Letters},
  title        = {Molecular cloning and sequence analysis of cDNA coding for the precursor of the human cysteine proteinase inhibitor cystatin C},
  url          = {http://dx.doi.org/10.1016/0014-5793(87)80695-6},
  volume       = {216},
  year         = {1987},
}