Production, characterization and use of monoclonal antibodies against the major extracellular human cysteine proteinase inhibitors cystatin C and kininogen
Olafsson, Isleifur ; Löfberg, Helge LU ; Abrahamson, Magnus LU and Grubb, Anders LU
(1988)
In Scandinavian Journal of Clinical & Laboratory Investigation
48(6).
p.573-582
- Abstract
- Murine monoclonal antibodies against the major cysteine proteinase inhibitors of human biological fluids, cystatin C and kininogen, were produced. The cystatin C antibody, HCC3, with a Ka of 2times107 l/mol, increased the inhibition of papain by cystatin C and was suitable for use in immunoblotting, immunohistochemistry and in the construction of a sensitive sandwich enzyme immunoassay for quantification of cystatin C. It recognized not only free cystatin C but also cystatin C in complexes with cysteine proteinases. The kininogen antibody, HK4, was directed against the third, cysteine proteinase inhibitory domain of the heavy chain of kininogen (Ka=1times107 l/mol), but did not influence the papain inhibitory activity of kininogen. It... (More)
- Murine monoclonal antibodies against the major cysteine proteinase inhibitors of human biological fluids, cystatin C and kininogen, were produced. The cystatin C antibody, HCC3, with a Ka of 2times107 l/mol, increased the inhibition of papain by cystatin C and was suitable for use in immunoblotting, immunohistochemistry and in the construction of a sensitive sandwich enzyme immunoassay for quantification of cystatin C. It recognized not only free cystatin C but also cystatin C in complexes with cysteine proteinases. The kininogen antibody, HK4, was directed against the third, cysteine proteinase inhibitory domain of the heavy chain of kininogen (Ka=1times107 l/mol), but did not influence the papain inhibitory activity of kininogen. It reacted with free kininogen as well as kininogen in complex with cysteine proteinases. Both antibodies could be used for the production of specific immunosorbents. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1104397
- author
- Olafsson, Isleifur
; Löfberg, Helge
LU
; Abrahamson, Magnus
LU
and Grubb, Anders
LU
- organization
- publishing date
- 1988
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- amyloidosis, cysteine proteinase, enzyme inhibitor, immunological techniques
- in
- Scandinavian Journal of Clinical & Laboratory Investigation
- volume
- 48
- issue
- 6
- pages
- 573 - 582
- publisher
- Informa Healthcare
- external identifiers
-
- scopus:0023784037
- ISSN
- 1502-7686
- DOI
- 10.3109/00365518809085775
- language
- English
- LU publication?
- yes
- id
- a8978142-2f76-4d18-96f1-16efd0131bc4 (old id 1104397)
- date added to LUP
- 2016-04-01 17:05:52
- date last changed
- 2021-01-03 05:43:47
@article{a8978142-2f76-4d18-96f1-16efd0131bc4,
abstract = {{Murine monoclonal antibodies against the major cysteine proteinase inhibitors of human biological fluids, cystatin C and kininogen, were produced. The cystatin C antibody, HCC3, with a Ka of 2times107 l/mol, increased the inhibition of papain by cystatin C and was suitable for use in immunoblotting, immunohistochemistry and in the construction of a sensitive sandwich enzyme immunoassay for quantification of cystatin C. It recognized not only free cystatin C but also cystatin C in complexes with cysteine proteinases. The kininogen antibody, HK4, was directed against the third, cysteine proteinase inhibitory domain of the heavy chain of kininogen (Ka=1times107 l/mol), but did not influence the papain inhibitory activity of kininogen. It reacted with free kininogen as well as kininogen in complex with cysteine proteinases. Both antibodies could be used for the production of specific immunosorbents.}},
author = {{Olafsson, Isleifur and Löfberg, Helge and Abrahamson, Magnus and Grubb, Anders}},
issn = {{1502-7686}},
keywords = {{amyloidosis; cysteine proteinase; enzyme inhibitor; immunological techniques}},
language = {{eng}},
number = {{6}},
pages = {{573--582}},
publisher = {{Informa Healthcare}},
series = {{Scandinavian Journal of Clinical & Laboratory Investigation}},
title = {{Production, characterization and use of monoclonal antibodies against the major extracellular human cysteine proteinase inhibitors cystatin C and kininogen}},
url = {{http://dx.doi.org/10.3109/00365518809085775}},
doi = {{10.3109/00365518809085775}},
volume = {{48}},
year = {{1988}},
}