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Production, characterization and use of monoclonal antibodies against the major extracellular human cysteine proteinase inhibitors cystatin C and kininogen

Olafsson, Isleifur; Löfberg, Helge LU ; Abrahamson, Magnus LU and Grubb, Anders LU (1988) In Scandinavian Journal of Clinical & Laboratory Investigation 48(6). p.573-582
Abstract
Murine monoclonal antibodies against the major cysteine proteinase inhibitors of human biological fluids, cystatin C and kininogen, were produced. The cystatin C antibody, HCC3, with a Ka of 2times107 l/mol, increased the inhibition of papain by cystatin C and was suitable for use in immunoblotting, immunohistochemistry and in the construction of a sensitive sandwich enzyme immunoassay for quantification of cystatin C. It recognized not only free cystatin C but also cystatin C in complexes with cysteine proteinases. The kininogen antibody, HK4, was directed against the third, cysteine proteinase inhibitory domain of the heavy chain of kininogen (Ka=1times107 l/mol), but did not influence the papain inhibitory activity of kininogen. It... (More)
Murine monoclonal antibodies against the major cysteine proteinase inhibitors of human biological fluids, cystatin C and kininogen, were produced. The cystatin C antibody, HCC3, with a Ka of 2times107 l/mol, increased the inhibition of papain by cystatin C and was suitable for use in immunoblotting, immunohistochemistry and in the construction of a sensitive sandwich enzyme immunoassay for quantification of cystatin C. It recognized not only free cystatin C but also cystatin C in complexes with cysteine proteinases. The kininogen antibody, HK4, was directed against the third, cysteine proteinase inhibitory domain of the heavy chain of kininogen (Ka=1times107 l/mol), but did not influence the papain inhibitory activity of kininogen. It reacted with free kininogen as well as kininogen in complex with cysteine proteinases. Both antibodies could be used for the production of specific immunosorbents. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
amyloidosis, cysteine proteinase, enzyme inhibitor, immunological techniques
in
Scandinavian Journal of Clinical & Laboratory Investigation
volume
48
issue
6
pages
573 - 582
publisher
Informa Healthcare
external identifiers
  • scopus:0023784037
ISSN
1502-7686
DOI
10.3109/00365518809085775
language
English
LU publication?
yes
id
a8978142-2f76-4d18-96f1-16efd0131bc4 (old id 1104397)
date added to LUP
2008-08-08 09:56:29
date last changed
2017-10-15 04:14:13
@article{a8978142-2f76-4d18-96f1-16efd0131bc4,
  abstract     = {Murine monoclonal antibodies against the major cysteine proteinase inhibitors of human biological fluids, cystatin C and kininogen, were produced. The cystatin C antibody, HCC3, with a Ka of 2times107 l/mol, increased the inhibition of papain by cystatin C and was suitable for use in immunoblotting, immunohistochemistry and in the construction of a sensitive sandwich enzyme immunoassay for quantification of cystatin C. It recognized not only free cystatin C but also cystatin C in complexes with cysteine proteinases. The kininogen antibody, HK4, was directed against the third, cysteine proteinase inhibitory domain of the heavy chain of kininogen (Ka=1times107 l/mol), but did not influence the papain inhibitory activity of kininogen. It reacted with free kininogen as well as kininogen in complex with cysteine proteinases. Both antibodies could be used for the production of specific immunosorbents.},
  author       = {Olafsson, Isleifur and Löfberg, Helge and Abrahamson, Magnus and Grubb, Anders},
  issn         = {1502-7686},
  keyword      = {amyloidosis,cysteine proteinase,enzyme inhibitor,immunological techniques},
  language     = {eng},
  number       = {6},
  pages        = {573--582},
  publisher    = {Informa Healthcare},
  series       = {Scandinavian Journal of Clinical & Laboratory Investigation},
  title        = {Production, characterization and use of monoclonal antibodies against the major extracellular human cysteine proteinase inhibitors cystatin C and kininogen},
  url          = {http://dx.doi.org/10.3109/00365518809085775},
  volume       = {48},
  year         = {1988},
}