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Evaluation of four qualitative methods for detection of beta-lactamase production in Staphylococcus and Micrococcus species

Petersson, A C; Eliasson, Ingvar LU ; Kamme, C and Miörner, Håkan LU (1989) In European Journal of Clinical Microbiology & Infectious Diseases 8(11). p.962-967
Abstract
Four qualitative methods for the detection of beta-lactamase production in Staphylococcus and Micrococcus species were evaluated and compared with a quantitative macroiodometric reference method. The disc diffusion test with penicillin G and the cloverleaf method could not separate beta-lactamase-positive from beta-lactamase-negative strains. Two applications of the chromogenic cephalosporin test, using uninduced strains and strains grown on blood agar plates, gave a large number of false negative and false positive results. False negative reactions were most common among uninduced strains, while the false positive reactions were most often recorded for Staphylococcus saprophyticus. A high degree of efficiency was recorded for the... (More)
Four qualitative methods for the detection of beta-lactamase production in Staphylococcus and Micrococcus species were evaluated and compared with a quantitative macroiodometric reference method. The disc diffusion test with penicillin G and the cloverleaf method could not separate beta-lactamase-positive from beta-lactamase-negative strains. Two applications of the chromogenic cephalosporin test, using uninduced strains and strains grown on blood agar plates, gave a large number of false negative and false positive results. False negative reactions were most common among uninduced strains, while the false positive reactions were most often recorded for Staphylococcus saprophyticus. A high degree of efficiency was recorded for the nitrocefin spot test, using induced strains grown on antibiotic susceptibility agar, and for the starch-iodine plate method. The starch-iodine plate with methicillin as inducer gave the most reliable results. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
European Journal of Clinical Microbiology & Infectious Diseases
volume
8
issue
11
pages
962 - 967
publisher
Springer
external identifiers
  • pmid:2513196
  • scopus:0024351539
ISSN
1435-4373
DOI
10.1007/BF01967566
language
English
LU publication?
yes
id
7fb96577-46ba-4de6-8791-54c72ea71182 (old id 1104598)
date added to LUP
2008-08-06 11:36:05
date last changed
2017-10-29 04:23:19
@article{7fb96577-46ba-4de6-8791-54c72ea71182,
  abstract     = {Four qualitative methods for the detection of beta-lactamase production in Staphylococcus and Micrococcus species were evaluated and compared with a quantitative macroiodometric reference method. The disc diffusion test with penicillin G and the cloverleaf method could not separate beta-lactamase-positive from beta-lactamase-negative strains. Two applications of the chromogenic cephalosporin test, using uninduced strains and strains grown on blood agar plates, gave a large number of false negative and false positive results. False negative reactions were most common among uninduced strains, while the false positive reactions were most often recorded for Staphylococcus saprophyticus. A high degree of efficiency was recorded for the nitrocefin spot test, using induced strains grown on antibiotic susceptibility agar, and for the starch-iodine plate method. The starch-iodine plate with methicillin as inducer gave the most reliable results.},
  author       = {Petersson, A C and Eliasson, Ingvar and Kamme, C and Miörner, Håkan},
  issn         = {1435-4373},
  language     = {eng},
  number       = {11},
  pages        = {962--967},
  publisher    = {Springer},
  series       = {European Journal of Clinical Microbiology & Infectious Diseases},
  title        = {Evaluation of four qualitative methods for detection of beta-lactamase production in Staphylococcus and Micrococcus species},
  url          = {http://dx.doi.org/10.1007/BF01967566},
  volume       = {8},
  year         = {1989},
}