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Ethanol stimulates basal and serotonin-induced formation of [32P]phosphatidic acid in human platelets

Simonsson, Per LU ; Ferencz, Istvan LU and Alling, Christer LU (1989) In Drug and Alcohol Dependence 24(2). p.169-174
Abstract
The addition of serotonin to preparations of 32P-labelled human platelets resulted in a time- and dose-dependent hydrolysis of [32P]phosphatidylinositol 4,5-bisphosphate (PIP2) and formation of [32P]phosphatidic acid (PA). This response was inhibited by the serotonin2 receptor antagonist ritanserin, indicating that the stimulation was mediated via the serotonin2 receptor. The addition of 50-150 mM of ethanol prior to stimulation with 10(-5) M serotonin resulted in an increased accumulation of [32P]PA, but had no effect on [32P]PIP2. Ethanol stimulated [32P]PA formation at all serotonin concentrations studied (10(-7)-10(-5) M). Furthermore, in the absence of serotonin, ethanol increased basal [32P]PA formation.
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ethanol, phospholipase C, serotonin, phosphatidic acid, platelets
in
Drug and Alcohol Dependence
volume
24
issue
2
pages
169 - 174
publisher
Elsevier
external identifiers
  • pmid:2791892
  • scopus:0024452304
ISSN
1879-0046
DOI
10.1016/0376-8716(89)90081-1
language
English
LU publication?
yes
id
53150406-c283-48b2-9fda-a23a58d37a44 (old id 1104753)
date added to LUP
2016-04-01 12:31:16
date last changed
2021-01-03 07:17:53
@article{53150406-c283-48b2-9fda-a23a58d37a44,
  abstract     = {{The addition of serotonin to preparations of 32P-labelled human platelets resulted in a time- and dose-dependent hydrolysis of [32P]phosphatidylinositol 4,5-bisphosphate (PIP2) and formation of [32P]phosphatidic acid (PA). This response was inhibited by the serotonin2 receptor antagonist ritanserin, indicating that the stimulation was mediated via the serotonin2 receptor. The addition of 50-150 mM of ethanol prior to stimulation with 10(-5) M serotonin resulted in an increased accumulation of [32P]PA, but had no effect on [32P]PIP2. Ethanol stimulated [32P]PA formation at all serotonin concentrations studied (10(-7)-10(-5) M). Furthermore, in the absence of serotonin, ethanol increased basal [32P]PA formation.}},
  author       = {{Simonsson, Per and Ferencz, Istvan and Alling, Christer}},
  issn         = {{1879-0046}},
  keywords     = {{ethanol; phospholipase C; serotonin; phosphatidic acid; platelets}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{169--174}},
  publisher    = {{Elsevier}},
  series       = {{Drug and Alcohol Dependence}},
  title        = {{Ethanol stimulates basal and serotonin-induced formation of [32P]phosphatidic acid in human platelets}},
  url          = {{http://dx.doi.org/10.1016/0376-8716(89)90081-1}},
  doi          = {{10.1016/0376-8716(89)90081-1}},
  volume       = {{24}},
  year         = {{1989}},
}