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Ethanol stimulates basal and serotonin-induced formation of [32P]phosphatidic acid in human platelets

Simonsson, Per LU ; Ferencz, Istvan LU and Alling, Christer LU (1989) In Drug and Alcohol Dependence 24(2). p.169-174
Abstract
The addition of serotonin to preparations of 32P-labelled human platelets resulted in a time- and dose-dependent hydrolysis of [32P]phosphatidylinositol 4,5-bisphosphate (PIP2) and formation of [32P]phosphatidic acid (PA). This response was inhibited by the serotonin2 receptor antagonist ritanserin, indicating that the stimulation was mediated via the serotonin2 receptor. The addition of 50-150 mM of ethanol prior to stimulation with 10(-5) M serotonin resulted in an increased accumulation of [32P]PA, but had no effect on [32P]PIP2. Ethanol stimulated [32P]PA formation at all serotonin concentrations studied (10(-7)-10(-5) M). Furthermore, in the absence of serotonin, ethanol increased basal [32P]PA formation.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ethanol, phospholipase C, serotonin, phosphatidic acid, platelets
in
Drug and Alcohol Dependence
volume
24
issue
2
pages
169 - 174
publisher
Elsevier
external identifiers
  • pmid:2791892
  • scopus:0024452304
ISSN
1879-0046
DOI
10.1016/0376-8716(89)90081-1
language
English
LU publication?
yes
id
53150406-c283-48b2-9fda-a23a58d37a44 (old id 1104753)
date added to LUP
2008-08-06 14:32:43
date last changed
2017-01-01 05:13:01
@article{53150406-c283-48b2-9fda-a23a58d37a44,
  abstract     = {The addition of serotonin to preparations of 32P-labelled human platelets resulted in a time- and dose-dependent hydrolysis of [32P]phosphatidylinositol 4,5-bisphosphate (PIP2) and formation of [32P]phosphatidic acid (PA). This response was inhibited by the serotonin2 receptor antagonist ritanserin, indicating that the stimulation was mediated via the serotonin2 receptor. The addition of 50-150 mM of ethanol prior to stimulation with 10(-5) M serotonin resulted in an increased accumulation of [32P]PA, but had no effect on [32P]PIP2. Ethanol stimulated [32P]PA formation at all serotonin concentrations studied (10(-7)-10(-5) M). Furthermore, in the absence of serotonin, ethanol increased basal [32P]PA formation.},
  author       = {Simonsson, Per and Ferencz, Istvan and Alling, Christer},
  issn         = {1879-0046},
  keyword      = {ethanol,phospholipase C,serotonin,phosphatidic acid,platelets},
  language     = {eng},
  number       = {2},
  pages        = {169--174},
  publisher    = {Elsevier},
  series       = {Drug and Alcohol Dependence},
  title        = {Ethanol stimulates basal and serotonin-induced formation of [32P]phosphatidic acid in human platelets},
  url          = {http://dx.doi.org/10.1016/0376-8716(89)90081-1},
  volume       = {24},
  year         = {1989},
}