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Method for ultrastructural studies of the intact tissue-metal interface

Bjursten, Lars Magnus LU ; Emanuelsson, L; Ericson, L E; Thomsen, P; Lausmaa, J; Mattsson, L; Rolander, U and Kasemo, B (1990) In Biomaterials 11(8). p.596-601
Abstract
Samples were prepared for ultrastructural studies of the intact interface between metallic implants and tissue by transmission electron microscopy. The method is based on plastic embedding of implant and tissue and subsequent removal of the bulk metal by electrochemical dissolution (electropolishing), to facilitate preparation of ultrathin sections for transmission electron microscopy. Surface sensitive spectroscopy (Auger electron microscopy and X-ray photoemission spectroscopy) and transmission electron microscopy EDX results show that the method produces samples with an intact interface, containing the implant surface oxide and the adjacent tissue. Examples of application of the method on titanium, zirconium and aluminium implants in... (More)
Samples were prepared for ultrastructural studies of the intact interface between metallic implants and tissue by transmission electron microscopy. The method is based on plastic embedding of implant and tissue and subsequent removal of the bulk metal by electrochemical dissolution (electropolishing), to facilitate preparation of ultrathin sections for transmission electron microscopy. Surface sensitive spectroscopy (Auger electron microscopy and X-ray photoemission spectroscopy) and transmission electron microscopy EDX results show that the method produces samples with an intact interface, containing the implant surface oxide and the adjacent tissue. Examples of application of the method on titanium, zirconium and aluminium implants in soft tissue are given. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Titanium, cell-material interface, ultrastructure
in
Biomaterials
volume
11
issue
8
pages
596 - 601
publisher
Elsevier
external identifiers
  • pmid:2279062
  • scopus:0025163480
ISSN
1878-5905
DOI
10.1016/0142-9612(90)90085-5
language
English
LU publication?
no
id
44f0fe49-e167-4e9a-9cc9-22f135de2d3e (old id 1104944)
date added to LUP
2008-08-04 14:22:50
date last changed
2017-08-20 03:44:45
@article{44f0fe49-e167-4e9a-9cc9-22f135de2d3e,
  abstract     = {Samples were prepared for ultrastructural studies of the intact interface between metallic implants and tissue by transmission electron microscopy. The method is based on plastic embedding of implant and tissue and subsequent removal of the bulk metal by electrochemical dissolution (electropolishing), to facilitate preparation of ultrathin sections for transmission electron microscopy. Surface sensitive spectroscopy (Auger electron microscopy and X-ray photoemission spectroscopy) and transmission electron microscopy EDX results show that the method produces samples with an intact interface, containing the implant surface oxide and the adjacent tissue. Examples of application of the method on titanium, zirconium and aluminium implants in soft tissue are given.},
  author       = {Bjursten, Lars Magnus and Emanuelsson, L and Ericson, L E and Thomsen, P and Lausmaa, J and Mattsson, L and Rolander, U and Kasemo, B},
  issn         = {1878-5905},
  keyword      = {Titanium,cell-material interface,ultrastructure},
  language     = {eng},
  number       = {8},
  pages        = {596--601},
  publisher    = {Elsevier},
  series       = {Biomaterials},
  title        = {Method for ultrastructural studies of the intact tissue-metal interface},
  url          = {http://dx.doi.org/10.1016/0142-9612(90)90085-5},
  volume       = {11},
  year         = {1990},
}