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Levels of neutrophil elastase and cathepsin B activities, and cystatins in human sputum: relationship to inflammation

Buttle, D J; Burnett, D and Abrahamson, Magnus LU (1990) In Scandinavian Journal of Clinical & Laboratory Investigation 50(5). p.509-516
Abstract
Sputum samples from 25 patients with bronchiectasis were assayed enzymatically for myeloperoxidase, neutrophil elastase and cathepsin B, and immunologically for cystatin A, cystatin B, cystatin C, cystatin S and kininogen. High myeloperoxidase and neutrophil elastase levels were found in those sputum samples that were assessed visually to be purulent. These samples were also found to contain high levels of cathepsin B activity and cystatin A, but low levels of cystatin S and of the most effective cathepsin B inhibitor, cystatin C. In contrast, sputum samples that were low in myeloperoxidase and neutrophil elastase activities had low levels of cathepsin B and cystatin A, but high cystatin C and S levels. It is concluded that cathepsin B... (More)
Sputum samples from 25 patients with bronchiectasis were assayed enzymatically for myeloperoxidase, neutrophil elastase and cathepsin B, and immunologically for cystatin A, cystatin B, cystatin C, cystatin S and kininogen. High myeloperoxidase and neutrophil elastase levels were found in those sputum samples that were assessed visually to be purulent. These samples were also found to contain high levels of cathepsin B activity and cystatin A, but low levels of cystatin S and of the most effective cathepsin B inhibitor, cystatin C. In contrast, sputum samples that were low in myeloperoxidase and neutrophil elastase activities had low levels of cathepsin B and cystatin A, but high cystatin C and S levels. It is concluded that cathepsin B activity in sputum is positively correlated with the degree of inflammation and neutrophil recruitment. Although this may be due in part to reduced amounts of cathepsin B inhibitors, particularly cystatin C, theoretical considerations suggest that factors other than the gross level of inhibitors must be involved in the control of cathepsin B activity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
agr2-macroglobulin, bronchiectasis, myeloperoxidase, neutrophil degranulation
in
Scandinavian Journal of Clinical & Laboratory Investigation
volume
50
issue
5
pages
509 - 516
publisher
Informa Healthcare
external identifiers
  • pmid:2237263
  • scopus:0025105872
ISSN
1502-7686
DOI
10.3109/00365519009089165
language
English
LU publication?
yes
id
8918d1f0-9c0f-4d53-b7ce-6ec0b2e32f35 (old id 1105552)
date added to LUP
2008-08-06 08:15:35
date last changed
2017-08-27 05:23:25
@article{8918d1f0-9c0f-4d53-b7ce-6ec0b2e32f35,
  abstract     = {Sputum samples from 25 patients with bronchiectasis were assayed enzymatically for myeloperoxidase, neutrophil elastase and cathepsin B, and immunologically for cystatin A, cystatin B, cystatin C, cystatin S and kininogen. High myeloperoxidase and neutrophil elastase levels were found in those sputum samples that were assessed visually to be purulent. These samples were also found to contain high levels of cathepsin B activity and cystatin A, but low levels of cystatin S and of the most effective cathepsin B inhibitor, cystatin C. In contrast, sputum samples that were low in myeloperoxidase and neutrophil elastase activities had low levels of cathepsin B and cystatin A, but high cystatin C and S levels. It is concluded that cathepsin B activity in sputum is positively correlated with the degree of inflammation and neutrophil recruitment. Although this may be due in part to reduced amounts of cathepsin B inhibitors, particularly cystatin C, theoretical considerations suggest that factors other than the gross level of inhibitors must be involved in the control of cathepsin B activity.},
  author       = {Buttle, D J and Burnett, D and Abrahamson, Magnus},
  issn         = {1502-7686},
  keyword      = {agr2-macroglobulin,bronchiectasis,myeloperoxidase,neutrophil degranulation},
  language     = {eng},
  number       = {5},
  pages        = {509--516},
  publisher    = {Informa Healthcare},
  series       = {Scandinavian Journal of Clinical & Laboratory Investigation},
  title        = {Levels of neutrophil elastase and cathepsin B activities, and cystatins in human sputum: relationship to inflammation},
  url          = {http://dx.doi.org/10.3109/00365519009089165},
  volume       = {50},
  year         = {1990},
}