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Interactions of papaya proteinase IV with inhibitors

Buttle, David J; Ritonja, Anka; Dando, Pamela M; Abrahamson, Magnus LU ; Shaw, Elliot N; Wikstrom, Peter; Turk, Vito and Barrett, Alan J (1990) In FEBS Letters 262(1). p.58-60
Abstract
Papaya proteinase IV (PPIV) is not inhibited by chicken cystatin, or human cystatins A or C, unlike most other proteinases of the papain superfamily. The enzyme inactivates chicken cystatin and human cystatin C by limited proteolysis of the glycyl bond previously shown to be involved in the inhibitory inactivity of the cystatins, but has no action on cystatin A. Contamination of commercial crystalline papain with PPIV accounts for the limited proteolysis of cystatins by ‘papain’ reported previously. PPIV is slowly bound by human α2-macroglobulin. The enzyme is irreversibly inactivated by E-64, and by peptidyl diazomethanes containing glycine in P1 and a hydrophobic side-chain in P2. The reaction of PPIV with iodoacetate is extremely slow.... (More)
Papaya proteinase IV (PPIV) is not inhibited by chicken cystatin, or human cystatins A or C, unlike most other proteinases of the papain superfamily. The enzyme inactivates chicken cystatin and human cystatin C by limited proteolysis of the glycyl bond previously shown to be involved in the inhibitory inactivity of the cystatins, but has no action on cystatin A. Contamination of commercial crystalline papain with PPIV accounts for the limited proteolysis of cystatins by ‘papain’ reported previously. PPIV is slowly bound by human α2-macroglobulin. The enzyme is irreversibly inactivated by E-64, and by peptidyl diazomethanes containing glycine in P1 and a hydrophobic side-chain in P2. The reaction of PPIV with iodoacetate is extremely slow. PPIV is inhibited by peptide aldehydes despite the presence of bulky sidechains in P1, suggesting that these reversible inhibitors do not bind as substrate analogues. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Macroglobulin, α2-, Cystatin, Compound E-64, Iodoacetate, Iodoacetamide, Papain, Peptide aldehyde, Peptidyl diazomethane
in
FEBS Letters
volume
262
issue
1
pages
58 - 60
publisher
Wiley-Blackwell
external identifiers
  • scopus:0025214927
ISSN
1873-3468
DOI
10.1016/0014-5793(90)80153-A
language
English
LU publication?
yes
id
fccddff6-4b6c-4793-9724-92b0d18c8e88 (old id 1105562)
date added to LUP
2008-08-06 08:21:58
date last changed
2017-08-06 04:23:29
@article{fccddff6-4b6c-4793-9724-92b0d18c8e88,
  abstract     = {Papaya proteinase IV (PPIV) is not inhibited by chicken cystatin, or human cystatins A or C, unlike most other proteinases of the papain superfamily. The enzyme inactivates chicken cystatin and human cystatin C by limited proteolysis of the glycyl bond previously shown to be involved in the inhibitory inactivity of the cystatins, but has no action on cystatin A. Contamination of commercial crystalline papain with PPIV accounts for the limited proteolysis of cystatins by ‘papain’ reported previously. PPIV is slowly bound by human α2-macroglobulin. The enzyme is irreversibly inactivated by E-64, and by peptidyl diazomethanes containing glycine in P1 and a hydrophobic side-chain in P2. The reaction of PPIV with iodoacetate is extremely slow. PPIV is inhibited by peptide aldehydes despite the presence of bulky sidechains in P1, suggesting that these reversible inhibitors do not bind as substrate analogues.},
  author       = {Buttle, David J and Ritonja, Anka and Dando, Pamela M and Abrahamson, Magnus and Shaw, Elliot N and Wikstrom, Peter and Turk, Vito and Barrett, Alan J},
  issn         = {1873-3468},
  keyword      = {Macroglobulin,α2-,Cystatin,Compound E-64,Iodoacetate,Iodoacetamide,Papain,Peptide aldehyde,Peptidyl diazomethane},
  language     = {eng},
  number       = {1},
  pages        = {58--60},
  publisher    = {Wiley-Blackwell},
  series       = {FEBS Letters},
  title        = {Interactions of papaya proteinase IV with inhibitors},
  url          = {http://dx.doi.org/10.1016/0014-5793(90)80153-A},
  volume       = {262},
  year         = {1990},
}