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Assignment of allotypes G1m(a+) and G1m(a-) at the genomic level by polymerase chain reaction analysis

Grubb, R; Abrahamson, Magnus LU and Grubb, Anders LU (1990) In Experimental and Clinical Immunogenetics 7(4). p.205-212
Abstract
A method of assignment of the human immunoglobulin allotypes G1m(a+) and G1m(a-) without the use of serological reagents is described. It is based upon oligonucleotide-directed enzymatic amplification of genomic segments encoding CH3 of gamma chains, followed by dot-blot hybridization of radioactively labelled oligonucleotides to the amplified DNA. The method was used to classify the immunoglobulin allotypes of 11 persons, six G1m(a+) and five G1m(a-), and the resultant classification agreed completely with that of classical serological typing.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Experimental and Clinical Immunogenetics
volume
7
issue
4
pages
205 - 212
publisher
Karger
external identifiers
  • pmid:2083093
  • scopus:0025642927
ISSN
0254-9670
language
English
LU publication?
yes
id
12d48977-85da-4aae-8789-13144a1b83d3 (old id 1105570)
date added to LUP
2008-08-06 08:25:04
date last changed
2017-01-01 06:54:34
@article{12d48977-85da-4aae-8789-13144a1b83d3,
  abstract     = {A method of assignment of the human immunoglobulin allotypes G1m(a+) and G1m(a-) without the use of serological reagents is described. It is based upon oligonucleotide-directed enzymatic amplification of genomic segments encoding CH3 of gamma chains, followed by dot-blot hybridization of radioactively labelled oligonucleotides to the amplified DNA. The method was used to classify the immunoglobulin allotypes of 11 persons, six G1m(a+) and five G1m(a-), and the resultant classification agreed completely with that of classical serological typing.},
  author       = {Grubb, R and Abrahamson, Magnus and Grubb, Anders},
  issn         = {0254-9670},
  language     = {eng},
  number       = {4},
  pages        = {205--212},
  publisher    = {Karger},
  series       = {Experimental and Clinical Immunogenetics},
  title        = {Assignment of allotypes G1m(a+) and G1m(a-) at the genomic level by polymerase chain reaction analysis},
  volume       = {7},
  year         = {1990},
}