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Assignment of allotypes G1m(a+) and G1m(a-) at the genomic level by polymerase chain reaction analysis

Grubb, R ; Abrahamson, Magnus LU and Grubb, Anders LU orcid (1990) In Experimental and Clinical Immunogenetics 7(4). p.205-212
Abstract
A method of assignment of the human immunoglobulin allotypes G1m(a+) and G1m(a-) without the use of serological reagents is described. It is based upon oligonucleotide-directed enzymatic amplification of genomic segments encoding CH3 of gamma chains, followed by dot-blot hybridization of radioactively labelled oligonucleotides to the amplified DNA. The method was used to classify the immunoglobulin allotypes of 11 persons, six G1m(a+) and five G1m(a-), and the resultant classification agreed completely with that of classical serological typing.
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Experimental and Clinical Immunogenetics
volume
7
issue
4
pages
205 - 212
publisher
Karger
external identifiers
  • pmid:2083093
  • scopus:0025642927
ISSN
0254-9670
language
English
LU publication?
yes
id
12d48977-85da-4aae-8789-13144a1b83d3 (old id 1105570)
date added to LUP
2016-04-01 16:03:54
date last changed
2021-01-03 05:26:18
@article{12d48977-85da-4aae-8789-13144a1b83d3,
  abstract     = {{A method of assignment of the human immunoglobulin allotypes G1m(a+) and G1m(a-) without the use of serological reagents is described. It is based upon oligonucleotide-directed enzymatic amplification of genomic segments encoding CH3 of gamma chains, followed by dot-blot hybridization of radioactively labelled oligonucleotides to the amplified DNA. The method was used to classify the immunoglobulin allotypes of 11 persons, six G1m(a+) and five G1m(a-), and the resultant classification agreed completely with that of classical serological typing.}},
  author       = {{Grubb, R and Abrahamson, Magnus and Grubb, Anders}},
  issn         = {{0254-9670}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{205--212}},
  publisher    = {{Karger}},
  series       = {{Experimental and Clinical Immunogenetics}},
  title        = {{Assignment of allotypes G1m(a+) and G1m(a-) at the genomic level by polymerase chain reaction analysis}},
  volume       = {{7}},
  year         = {{1990}},
}