Advanced

Method for the biological monitoring of hexahydrophthalic anhydride by the determination of hexahydrophthalic acid in urine using gas chromatography and selected-ion monitoring

Jönsson, Bo A LU and Skarping, Gunnar LU (1991) In Journal of Chromatography A 572(1-2). p.117-131
Abstract
A method for the determination of hexahydrophthalic acid, a metabolite of hexahydrophthalic anhydride, in human urine has been developed. The urine was worked-up by liquid-solid extraction, esterified with boron trifluoride-methanol, and analysed by capillary gas chromatography and selected-ion monitoring. Hexadeuterium-labelled hexahydrophthalic acid was used as the internal standard. The precision was 4% at 0.7 microgram/ml and 5% at 0.07 microgram/ml. The recovery of the acid for the overall method was 101% at 0.07 micrograms/ml of urine (with a coefficient of variation of 4%) and 95% at 0.7 microgram/ml (coefficient of variation 2%). The limit of detection was 20 ng/ml urine.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Chromatography A
volume
572
issue
1-2
pages
117 - 131
publisher
Elsevier
external identifiers
  • pmid:1818048
  • scopus:0026323853
ISSN
0021-9673
language
English
LU publication?
yes
id
fa172fcb-dec8-4188-9a6f-7864c79ddbff (old id 1105704)
date added to LUP
2008-08-04 10:02:04
date last changed
2017-01-01 07:17:36
@article{fa172fcb-dec8-4188-9a6f-7864c79ddbff,
  abstract     = {A method for the determination of hexahydrophthalic acid, a metabolite of hexahydrophthalic anhydride, in human urine has been developed. The urine was worked-up by liquid-solid extraction, esterified with boron trifluoride-methanol, and analysed by capillary gas chromatography and selected-ion monitoring. Hexadeuterium-labelled hexahydrophthalic acid was used as the internal standard. The precision was 4% at 0.7 microgram/ml and 5% at 0.07 microgram/ml. The recovery of the acid for the overall method was 101% at 0.07 micrograms/ml of urine (with a coefficient of variation of 4%) and 95% at 0.7 microgram/ml (coefficient of variation 2%). The limit of detection was 20 ng/ml urine.},
  author       = {Jönsson, Bo A and Skarping, Gunnar},
  issn         = {0021-9673},
  language     = {eng},
  number       = {1-2},
  pages        = {117--131},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Method for the biological monitoring of hexahydrophthalic anhydride by the determination of hexahydrophthalic acid in urine using gas chromatography and selected-ion monitoring},
  volume       = {572},
  year         = {1991},
}