Method for the biological monitoring of hexahydrophthalic anhydride by the determination of hexahydrophthalic acid in urine using gas chromatography and selected-ion monitoring
(1991) In Journal of Chromatography A 572(1-2). p.117-131- Abstract
- A method for the determination of hexahydrophthalic acid, a metabolite of hexahydrophthalic anhydride, in human urine has been developed. The urine was worked-up by liquid-solid extraction, esterified with boron trifluoride-methanol, and analysed by capillary gas chromatography and selected-ion monitoring. Hexadeuterium-labelled hexahydrophthalic acid was used as the internal standard. The precision was 4% at 0.7 microgram/ml and 5% at 0.07 microgram/ml. The recovery of the acid for the overall method was 101% at 0.07 micrograms/ml of urine (with a coefficient of variation of 4%) and 95% at 0.7 microgram/ml (coefficient of variation 2%). The limit of detection was 20 ng/ml urine.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1105704
- author
- Jönsson, Bo A LU and Skarping, Gunnar LU
- organization
- publishing date
- 1991
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Chromatography A
- volume
- 572
- issue
- 1-2
- pages
- 117 - 131
- publisher
- Elsevier
- external identifiers
-
- pmid:1818048
- scopus:0026323853
- ISSN
- 0021-9673
- language
- English
- LU publication?
- yes
- id
- fa172fcb-dec8-4188-9a6f-7864c79ddbff (old id 1105704)
- date added to LUP
- 2016-04-01 16:52:28
- date last changed
- 2021-01-03 11:31:33
@article{fa172fcb-dec8-4188-9a6f-7864c79ddbff, abstract = {{A method for the determination of hexahydrophthalic acid, a metabolite of hexahydrophthalic anhydride, in human urine has been developed. The urine was worked-up by liquid-solid extraction, esterified with boron trifluoride-methanol, and analysed by capillary gas chromatography and selected-ion monitoring. Hexadeuterium-labelled hexahydrophthalic acid was used as the internal standard. The precision was 4% at 0.7 microgram/ml and 5% at 0.07 microgram/ml. The recovery of the acid for the overall method was 101% at 0.07 micrograms/ml of urine (with a coefficient of variation of 4%) and 95% at 0.7 microgram/ml (coefficient of variation 2%). The limit of detection was 20 ng/ml urine.}}, author = {{Jönsson, Bo A and Skarping, Gunnar}}, issn = {{0021-9673}}, language = {{eng}}, number = {{1-2}}, pages = {{117--131}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography A}}, title = {{Method for the biological monitoring of hexahydrophthalic anhydride by the determination of hexahydrophthalic acid in urine using gas chromatography and selected-ion monitoring}}, volume = {{572}}, year = {{1991}}, }