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Protein D, an immunoglobulin D-binding protein of Haemophilus influenzae: cloning, nucleotide sequence, and expression in Escherichia coli

Janson, Håkan LU ; Hedén, Lars-Olof LU ; Grubb, Anders LU ; Ruan, Maorong and Forsgren, Arne LU (1991) In Infection and Immunity 59(1). p.119-125
Abstract
The gene for protein D, a membrane-associated protein with specific affinity for human immunoglobulin D, was cloned from a nontypeable strain of Haemophilus influenzae. The gene was expressed in Escherichia coli from an endogenous promoter, and the gene product has an apparent molecular weight equal to that of H. influenzae protein D (42,000). The complete nucleotide sequence of the gene for protein D was determined, and the deduced amino acid sequence of 364 residues includes a putative signal sequence of 18 amino acids containing a consensus sequence, Leu-Ala-Gly-Cys, for bacterial lipoproteins. The sequence of protein D shows no similarity to those of other immunoglobulin-binding proteins. Protein D is the first example of... (More)
The gene for protein D, a membrane-associated protein with specific affinity for human immunoglobulin D, was cloned from a nontypeable strain of Haemophilus influenzae. The gene was expressed in Escherichia coli from an endogenous promoter, and the gene product has an apparent molecular weight equal to that of H. influenzae protein D (42,000). The complete nucleotide sequence of the gene for protein D was determined, and the deduced amino acid sequence of 364 residues includes a putative signal sequence of 18 amino acids containing a consensus sequence, Leu-Ala-Gly-Cys, for bacterial lipoproteins. The sequence of protein D shows no similarity to those of other immunoglobulin-binding proteins. Protein D is the first example of immunoglobulin receptors from gram-negative bacteria that has been cloned and sequenced. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Infection and Immunity
volume
59
issue
1
pages
119 - 125
publisher
American Society for Microbiology
external identifiers
  • pmid:1987023
  • scopus:0025976328
ISSN
1098-5522
language
English
LU publication?
yes
id
7beb7331-3358-417f-8a40-8de50e6ea996 (old id 1105723)
alternative location
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=257714
http://iai.asm.org/cgi/reprint/59/1/119
date added to LUP
2008-08-04 10:23:17
date last changed
2017-07-30 03:53:23
@article{7beb7331-3358-417f-8a40-8de50e6ea996,
  abstract     = {The gene for protein D, a membrane-associated protein with specific affinity for human immunoglobulin D, was cloned from a nontypeable strain of Haemophilus influenzae. The gene was expressed in Escherichia coli from an endogenous promoter, and the gene product has an apparent molecular weight equal to that of H. influenzae protein D (42,000). The complete nucleotide sequence of the gene for protein D was determined, and the deduced amino acid sequence of 364 residues includes a putative signal sequence of 18 amino acids containing a consensus sequence, Leu-Ala-Gly-Cys, for bacterial lipoproteins. The sequence of protein D shows no similarity to those of other immunoglobulin-binding proteins. Protein D is the first example of immunoglobulin receptors from gram-negative bacteria that has been cloned and sequenced.},
  author       = {Janson, Håkan and Hedén, Lars-Olof and Grubb, Anders and Ruan, Maorong and Forsgren, Arne},
  issn         = {1098-5522},
  language     = {eng},
  number       = {1},
  pages        = {119--125},
  publisher    = {American Society for Microbiology},
  series       = {Infection and Immunity},
  title        = {Protein D, an immunoglobulin D-binding protein of Haemophilus influenzae: cloning, nucleotide sequence, and expression in Escherichia coli},
  volume       = {59},
  year         = {1991},
}