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Tumour necrosis factor-alpha interacts with biglycan and decorin.

Tufvesson, Ellen LU and Westergren-Thorsson, Gunilla LU (2002) In FEBS Letters 530(1-3). p.124-128
Abstract
Several interactions of cytokines with extracellular matrix molecules are mediated by proteoglycans, such as biglycan and decorin. Using surface plasmon resonance, we show for the first time that tumour necrosis factor-alpha (TNF-alpha) binds to both biglycan and decorin with K(d)s of 0.81 microM and 1.23 microM respectively, a binding that was confirmed by Scatchard plots using a solid phase assay. Binding occurs preferentially via the core protein, shown by lower K(d)s, 0.26 microM and 0.81 microM for biglycan and decorin respectively. There was also binding to dermatan sulphate, with a K(d) of 10.53 microM. The function of this interaction between TNF-alpha and biglycan and decorin is not known, but we suggest that the differential... (More)
Several interactions of cytokines with extracellular matrix molecules are mediated by proteoglycans, such as biglycan and decorin. Using surface plasmon resonance, we show for the first time that tumour necrosis factor-alpha (TNF-alpha) binds to both biglycan and decorin with K(d)s of 0.81 microM and 1.23 microM respectively, a binding that was confirmed by Scatchard plots using a solid phase assay. Binding occurs preferentially via the core protein, shown by lower K(d)s, 0.26 microM and 0.81 microM for biglycan and decorin respectively. There was also binding to dermatan sulphate, with a K(d) of 10.53 microM. The function of this interaction between TNF-alpha and biglycan and decorin is not known, but we suggest that the differential localisation of the proteoglycans enables the cytokines to be immobilised in different environments. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
FEBS Letters
volume
530
issue
1-3
pages
124 - 128
publisher
Wiley-Blackwell
external identifiers
  • pmid:12387878
  • wos:000178856900023
  • scopus:0037163862
ISSN
1873-3468
DOI
10.1016/S0014-5793(02)03439-7
language
English
LU publication?
yes
id
84646ce4-c58a-4896-839b-5ae9eeef1e63 (old id 110574)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12387878&dopt=Abstract
date added to LUP
2007-07-19 16:08:32
date last changed
2017-10-22 04:47:58
@article{84646ce4-c58a-4896-839b-5ae9eeef1e63,
  abstract     = {Several interactions of cytokines with extracellular matrix molecules are mediated by proteoglycans, such as biglycan and decorin. Using surface plasmon resonance, we show for the first time that tumour necrosis factor-alpha (TNF-alpha) binds to both biglycan and decorin with K(d)s of 0.81 microM and 1.23 microM respectively, a binding that was confirmed by Scatchard plots using a solid phase assay. Binding occurs preferentially via the core protein, shown by lower K(d)s, 0.26 microM and 0.81 microM for biglycan and decorin respectively. There was also binding to dermatan sulphate, with a K(d) of 10.53 microM. The function of this interaction between TNF-alpha and biglycan and decorin is not known, but we suggest that the differential localisation of the proteoglycans enables the cytokines to be immobilised in different environments.},
  author       = {Tufvesson, Ellen and Westergren-Thorsson, Gunilla},
  issn         = {1873-3468},
  language     = {eng},
  number       = {1-3},
  pages        = {124--128},
  publisher    = {Wiley-Blackwell},
  series       = {FEBS Letters},
  title        = {Tumour necrosis factor-alpha interacts with biglycan and decorin.},
  url          = {http://dx.doi.org/10.1016/S0014-5793(02)03439-7},
  volume       = {530},
  year         = {2002},
}