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G proteins coupled to phospholipase C: molecular targets of long-term ethanol exposure

Simonsson, Per LU ; Rodriguez, F David; Loman, Niklas LU and Alling, Christer LU (1991) In Journal of Neurochemistry 56(6). p.2018-2026
Abstract
Long-term ethanol exposure is known to inhibit bradykinin-stimulated phosphoinositide hydrolysis in cultures of neuroblastoma x glioma 108-15 cells. In the present study, [3H]bradykinin binding, GTP-binding protein function, and phospholipase C activity were assayed in cells grown for 4 days in 100 mM ethanol with the aim of elucidating the molecular target of ethanol on signal transduction coupled to inositol trisphosphate and diacylglycerol formation. Ethanol exposure reduced guanosine 5'-O-(3-thiotriphosphate) [GTP(S)]- and, to a lesser extent, NaF/AlCl3-stimulated phosphoinositide hydrolysis, whereas it had no effect on the enzymatic activity of a phosphatidylinositol 4,5-bisphosphate-specific phospholipase C. [3H]Bradykinin binding in... (More)
Long-term ethanol exposure is known to inhibit bradykinin-stimulated phosphoinositide hydrolysis in cultures of neuroblastoma x glioma 108-15 cells. In the present study, [3H]bradykinin binding, GTP-binding protein function, and phospholipase C activity were assayed in cells grown for 4 days in 100 mM ethanol with the aim of elucidating the molecular target of ethanol on signal transduction coupled to inositol trisphosphate and diacylglycerol formation. Ethanol exposure reduced guanosine 5'-O-(3-thiotriphosphate) [GTP(S)]- and, to a lesser extent, NaF/AlCl3-stimulated phosphoinositide hydrolysis, whereas it had no effect on the enzymatic activity of a phosphatidylinositol 4,5-bisphosphate-specific phospholipase C. [3H]Bradykinin binding in the absence of GTP(S) was not influenced by ethanol exposure. However, the reduction in [3H]bradykinin binding seen in control cells after addition of GTP analogue was inhibited in cells grown in ethanol-containing medium. The results indicate that long-term ethanol exposure exerts its effects on receptor-stimulated phosphoinositide hydrolysis primarily at the level of the GTP-binding protein. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bradykinin, Ethanol, Phosphatidylinositol 4, 5-bisphosphate, Phospholipase C, GTP-binding protein, NG 108–15 cell line
in
Journal of Neurochemistry
volume
56
issue
6
pages
2018 - 2026
publisher
Wiley-Blackwell
external identifiers
  • pmid:1851210
  • scopus:0025944450
ISSN
1471-4159
DOI
10.1111/j.1471-4159.1991.tb03461.x
language
English
LU publication?
yes
id
06b806dd-59bd-46f9-ac53-91501d5aa476 (old id 1105752)
date added to LUP
2008-08-04 10:53:06
date last changed
2017-01-01 07:00:38
@article{06b806dd-59bd-46f9-ac53-91501d5aa476,
  abstract     = {Long-term ethanol exposure is known to inhibit bradykinin-stimulated phosphoinositide hydrolysis in cultures of neuroblastoma x glioma 108-15 cells. In the present study, [3H]bradykinin binding, GTP-binding protein function, and phospholipase C activity were assayed in cells grown for 4 days in 100 mM ethanol with the aim of elucidating the molecular target of ethanol on signal transduction coupled to inositol trisphosphate and diacylglycerol formation. Ethanol exposure reduced guanosine 5'-O-(3-thiotriphosphate) [GTP(S)]- and, to a lesser extent, NaF/AlCl3-stimulated phosphoinositide hydrolysis, whereas it had no effect on the enzymatic activity of a phosphatidylinositol 4,5-bisphosphate-specific phospholipase C. [3H]Bradykinin binding in the absence of GTP(S) was not influenced by ethanol exposure. However, the reduction in [3H]bradykinin binding seen in control cells after addition of GTP analogue was inhibited in cells grown in ethanol-containing medium. The results indicate that long-term ethanol exposure exerts its effects on receptor-stimulated phosphoinositide hydrolysis primarily at the level of the GTP-binding protein.},
  author       = {Simonsson, Per and Rodriguez, F David and Loman, Niklas and Alling, Christer},
  issn         = {1471-4159},
  keyword      = {Bradykinin,Ethanol,Phosphatidylinositol 4,5-bisphosphate,Phospholipase C,GTP-binding protein,NG 108–15 cell line},
  language     = {eng},
  number       = {6},
  pages        = {2018--2026},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Neurochemistry},
  title        = {G proteins coupled to phospholipase C: molecular targets of long-term ethanol exposure},
  url          = {http://dx.doi.org/10.1111/j.1471-4159.1991.tb03461.x},
  volume       = {56},
  year         = {1991},
}