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Radio-iodinated and internally labelled (35S) IgM monoclonal antibodies in a syngenic rat model

Wanying, Q; Brodin, T; Ceberg, Crister LU ; Ingvar, Christian LU ; Norrgren, Kristina LU ; Sjogren, H O and Strand, Sven-Erik LU (1991) In Acta Oncologica 30(3). p.379-383
Abstract
To simulate the human situation concerning human monoclonal antibodies (MAbs), we have introduced a new syngenic rat model with an implanted rat colon carcinoma. Rat IgM MAbs (10B12), labelled by the chloramine-T method with 125I or internally with 35S, were injected intravenously into the rats and the biodistribution was studied for 8 days. The radioactivity uptake in the tumours of the 35S label was higher than that of the 125I label and the retention of 35S in the tumours gave tumour/blood ratios 8 times higher than those of 125I at 48 and 96 h after injection. In this model we have shown that dehalogenation of iodinated IgM MAbs is a serious problem. We therefore suggest that internally labelled MAbs should be used and that further... (More)
To simulate the human situation concerning human monoclonal antibodies (MAbs), we have introduced a new syngenic rat model with an implanted rat colon carcinoma. Rat IgM MAbs (10B12), labelled by the chloramine-T method with 125I or internally with 35S, were injected intravenously into the rats and the biodistribution was studied for 8 days. The radioactivity uptake in the tumours of the 35S label was higher than that of the 125I label and the retention of 35S in the tumours gave tumour/blood ratios 8 times higher than those of 125I at 48 and 96 h after injection. In this model we have shown that dehalogenation of iodinated IgM MAbs is a serious problem. We therefore suggest that internally labelled MAbs should be used and that further investigations should be carried out in a syngenic rat model, since this probably reflects the clinical situation better than the nude mouse model. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Monoclonal antibodies, colon carcinoma, rat
in
Acta Oncologica
volume
30
issue
3
pages
379 - 383
publisher
Taylor & Francis
external identifiers
  • pmid:2036250
  • scopus:0025790945
ISSN
1651-226X
DOI
10.3109/02841869109092390
language
English
LU publication?
yes
id
60ae7c3a-2dc4-40a9-9f7b-d4ac88040d85 (old id 1105770)
date added to LUP
2008-08-04 11:17:32
date last changed
2017-01-01 07:21:06
@article{60ae7c3a-2dc4-40a9-9f7b-d4ac88040d85,
  abstract     = {To simulate the human situation concerning human monoclonal antibodies (MAbs), we have introduced a new syngenic rat model with an implanted rat colon carcinoma. Rat IgM MAbs (10B12), labelled by the chloramine-T method with 125I or internally with 35S, were injected intravenously into the rats and the biodistribution was studied for 8 days. The radioactivity uptake in the tumours of the 35S label was higher than that of the 125I label and the retention of 35S in the tumours gave tumour/blood ratios 8 times higher than those of 125I at 48 and 96 h after injection. In this model we have shown that dehalogenation of iodinated IgM MAbs is a serious problem. We therefore suggest that internally labelled MAbs should be used and that further investigations should be carried out in a syngenic rat model, since this probably reflects the clinical situation better than the nude mouse model.},
  author       = {Wanying, Q and Brodin, T and Ceberg, Crister and Ingvar, Christian and Norrgren, Kristina and Sjogren, H O and Strand, Sven-Erik},
  issn         = {1651-226X},
  keyword      = {Monoclonal antibodies,colon carcinoma,rat},
  language     = {eng},
  number       = {3},
  pages        = {379--383},
  publisher    = {Taylor & Francis},
  series       = {Acta Oncologica},
  title        = {Radio-iodinated and internally labelled (35S) IgM monoclonal antibodies in a syngenic rat model},
  url          = {http://dx.doi.org/10.3109/02841869109092390},
  volume       = {30},
  year         = {1991},
}