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The influence of neuropeptide Y and norepinephrine on ovulation in the rat ovary

Jörgensen, Jörgen Ch; Kannisto, Päivi LU ; Liedberg, Fredrik LU ; Ottesen, Bent; Owman, Christer LU and Schmidt, Günter (1991) In Peptides 12(5). p.975-982
Abstract
Neuropeptide Y (NPY) was measured in tissue extracts from ovaries of rats treated with pregnant mare serum gonadotropin (PMSG). The extracted NPY-immunoreactive material was identical to synthetic human NPY with regard to size and hydrophobicity as evaluated by gel filtration and high performance liquid chromatography. The concentration of NPY was related to the estrous cycle and a maximum was observed in relation to the endogenous luteinizing hormone (LH) peak. NPY immunoreactivity was demonstrated by immunohistochemistry to be localized within nerve fibers supplying blood vessels and follicles. The increase in the NPY content could not be related to accumulation around specific ovarian structures. Employing an in vitro set-up, NPY... (More)
Neuropeptide Y (NPY) was measured in tissue extracts from ovaries of rats treated with pregnant mare serum gonadotropin (PMSG). The extracted NPY-immunoreactive material was identical to synthetic human NPY with regard to size and hydrophobicity as evaluated by gel filtration and high performance liquid chromatography. The concentration of NPY was related to the estrous cycle and a maximum was observed in relation to the endogenous luteinizing hormone (LH) peak. NPY immunoreactivity was demonstrated by immunohistochemistry to be localized within nerve fibers supplying blood vessels and follicles. The increase in the NPY content could not be related to accumulation around specific ovarian structures. Employing an in vitro set-up, NPY (10(-7) M) was unable to induce ovulation and did not increase the ovulation rate in LH-stimulated ovaries. The combination of NPY (10(-7) M) and NE (10(-7) M) did not significantly increase the number of ovulations compared to that induced by NE (10(-7) M) alone. In conclusion, NPY content in the ovary is related to the estrous cycle, but NPY does not seem to have any direct effect on the ovulatory process. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Neuropeptide Y, Norepinephrine, Tyrosine hydroxylase, Ovulation
in
Peptides
volume
12
issue
5
pages
975 - 982
publisher
Elsevier
external identifiers
  • pmid:1686937
  • scopus:0026014971
ISSN
1873-5169
DOI
10.1016/0196-9781(91)90046-R
language
English
LU publication?
yes
id
1a6b4b53-9d79-4082-a512-4627a2928d0f (old id 1105955)
date added to LUP
2008-08-01 11:50:23
date last changed
2017-02-22 11:44:10
@article{1a6b4b53-9d79-4082-a512-4627a2928d0f,
  abstract     = {Neuropeptide Y (NPY) was measured in tissue extracts from ovaries of rats treated with pregnant mare serum gonadotropin (PMSG). The extracted NPY-immunoreactive material was identical to synthetic human NPY with regard to size and hydrophobicity as evaluated by gel filtration and high performance liquid chromatography. The concentration of NPY was related to the estrous cycle and a maximum was observed in relation to the endogenous luteinizing hormone (LH) peak. NPY immunoreactivity was demonstrated by immunohistochemistry to be localized within nerve fibers supplying blood vessels and follicles. The increase in the NPY content could not be related to accumulation around specific ovarian structures. Employing an in vitro set-up, NPY (10(-7) M) was unable to induce ovulation and did not increase the ovulation rate in LH-stimulated ovaries. The combination of NPY (10(-7) M) and NE (10(-7) M) did not significantly increase the number of ovulations compared to that induced by NE (10(-7) M) alone. In conclusion, NPY content in the ovary is related to the estrous cycle, but NPY does not seem to have any direct effect on the ovulatory process.},
  author       = {Jörgensen, Jörgen Ch and Kannisto, Päivi and Liedberg, Fredrik and Ottesen, Bent and Owman, Christer and Schmidt, Günter},
  issn         = {1873-5169},
  keyword      = {Neuropeptide Y,Norepinephrine,Tyrosine hydroxylase,Ovulation},
  language     = {eng},
  number       = {5},
  pages        = {975--982},
  publisher    = {Elsevier},
  series       = {Peptides},
  title        = {The influence of neuropeptide Y and norepinephrine on ovulation in the rat ovary},
  url          = {http://dx.doi.org/10.1016/0196-9781(91)90046-R},
  volume       = {12},
  year         = {1991},
}