The influence of neuropeptide Y and norepinephrine on ovulation in the rat ovary
(1991) In Peptides 12(5). p.975-982- Abstract
- Neuropeptide Y (NPY) was measured in tissue extracts from ovaries of rats treated with pregnant mare serum gonadotropin (PMSG). The extracted NPY-immunoreactive material was identical to synthetic human NPY with regard to size and hydrophobicity as evaluated by gel filtration and high performance liquid chromatography. The concentration of NPY was related to the estrous cycle and a maximum was observed in relation to the endogenous luteinizing hormone (LH) peak. NPY immunoreactivity was demonstrated by immunohistochemistry to be localized within nerve fibers supplying blood vessels and follicles. The increase in the NPY content could not be related to accumulation around specific ovarian structures. Employing an in vitro set-up, NPY... (More)
- Neuropeptide Y (NPY) was measured in tissue extracts from ovaries of rats treated with pregnant mare serum gonadotropin (PMSG). The extracted NPY-immunoreactive material was identical to synthetic human NPY with regard to size and hydrophobicity as evaluated by gel filtration and high performance liquid chromatography. The concentration of NPY was related to the estrous cycle and a maximum was observed in relation to the endogenous luteinizing hormone (LH) peak. NPY immunoreactivity was demonstrated by immunohistochemistry to be localized within nerve fibers supplying blood vessels and follicles. The increase in the NPY content could not be related to accumulation around specific ovarian structures. Employing an in vitro set-up, NPY (10(-7) M) was unable to induce ovulation and did not increase the ovulation rate in LH-stimulated ovaries. The combination of NPY (10(-7) M) and NE (10(-7) M) did not significantly increase the number of ovulations compared to that induced by NE (10(-7) M) alone. In conclusion, NPY content in the ovary is related to the estrous cycle, but NPY does not seem to have any direct effect on the ovulatory process. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1105955
- author
- Jörgensen, Jörgen Ch ; Kannisto, Päivi LU ; Liedberg, Fredrik LU ; Ottesen, Bent ; Owman, Christer LU and Schmidt, Günter
- organization
- publishing date
- 1991
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Neuropeptide Y, Norepinephrine, Tyrosine hydroxylase, Ovulation
- in
- Peptides
- volume
- 12
- issue
- 5
- pages
- 975 - 982
- publisher
- Elsevier
- external identifiers
-
- pmid:1686937
- scopus:0026014971
- ISSN
- 1873-5169
- DOI
- 10.1016/0196-9781(91)90046-R
- language
- English
- LU publication?
- yes
- id
- 1a6b4b53-9d79-4082-a512-4627a2928d0f (old id 1105955)
- date added to LUP
- 2016-04-01 12:06:11
- date last changed
- 2021-01-03 08:47:48
@article{1a6b4b53-9d79-4082-a512-4627a2928d0f, abstract = {{Neuropeptide Y (NPY) was measured in tissue extracts from ovaries of rats treated with pregnant mare serum gonadotropin (PMSG). The extracted NPY-immunoreactive material was identical to synthetic human NPY with regard to size and hydrophobicity as evaluated by gel filtration and high performance liquid chromatography. The concentration of NPY was related to the estrous cycle and a maximum was observed in relation to the endogenous luteinizing hormone (LH) peak. NPY immunoreactivity was demonstrated by immunohistochemistry to be localized within nerve fibers supplying blood vessels and follicles. The increase in the NPY content could not be related to accumulation around specific ovarian structures. Employing an in vitro set-up, NPY (10(-7) M) was unable to induce ovulation and did not increase the ovulation rate in LH-stimulated ovaries. The combination of NPY (10(-7) M) and NE (10(-7) M) did not significantly increase the number of ovulations compared to that induced by NE (10(-7) M) alone. In conclusion, NPY content in the ovary is related to the estrous cycle, but NPY does not seem to have any direct effect on the ovulatory process.}}, author = {{Jörgensen, Jörgen Ch and Kannisto, Päivi and Liedberg, Fredrik and Ottesen, Bent and Owman, Christer and Schmidt, Günter}}, issn = {{1873-5169}}, keywords = {{Neuropeptide Y; Norepinephrine; Tyrosine hydroxylase; Ovulation}}, language = {{eng}}, number = {{5}}, pages = {{975--982}}, publisher = {{Elsevier}}, series = {{Peptides}}, title = {{The influence of neuropeptide Y and norepinephrine on ovulation in the rat ovary}}, url = {{http://dx.doi.org/10.1016/0196-9781(91)90046-R}}, doi = {{10.1016/0196-9781(91)90046-R}}, volume = {{12}}, year = {{1991}}, }