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Mechanics and Ca(2+)-sensitivity of human detrusor muscle bundles studied in vitro

Malmqvist, Ulf LU ; Arner, Anders LU and Uvelius, Bengt LU (1991) In Acta Physiologica Scandinavica 143(4). p.373-380
Abstract
Mechanical properties of isolated smooth muscle strips from human urinary bladder were investigated in vitro. Bladder tissue was obtained from tumour-free wall regions of bladders from male patients undergoing cystectomy for bladder carcinoma. In intact muscle strips, activated with high-K+ solution, half-maximal force occurred at about 0.9 mM extracellular [Ca2+]. The length-active force relation was determined and the muscle strips were fixed for light and electron microscopy at optimal length for active force (1o). The maximal active force per unit smooth muscle cross-sectional area was 208 +/- 49 mN/mm2, n = 6. Chemically skinned preparations were obtained by treatment with triton X-100. These preparations had a steep [Ca2+]-force... (More)
Mechanical properties of isolated smooth muscle strips from human urinary bladder were investigated in vitro. Bladder tissue was obtained from tumour-free wall regions of bladders from male patients undergoing cystectomy for bladder carcinoma. In intact muscle strips, activated with high-K+ solution, half-maximal force occurred at about 0.9 mM extracellular [Ca2+]. The length-active force relation was determined and the muscle strips were fixed for light and electron microscopy at optimal length for active force (1o). The maximal active force per unit smooth muscle cross-sectional area was 208 +/- 49 mN/mm2, n = 6. Chemically skinned preparations were obtained by treatment with triton X-100. These preparations had a steep [Ca2+]-force relation in the micromolar range which was influenced by calmodulin. The skinned preparations could be maximally activated by irreversible thiophosphorylation of the regulatory light chains. The force-velocity relation was determined in the maximally activated skinned muscle at 22 degrees C at 0.51o. When the muscle was shortened by 10%, force was reduced by 35% whereas the maximal shortening velocity was little affected. (Less)
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organization
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Contribution to journal
publication status
published
subject
in
Acta Physiologica Scandinavica
volume
143
issue
4
pages
373 - 380
publisher
Wiley-Blackwell
external identifiers
  • pmid:1815473
ISSN
0001-6772
language
English
LU publication?
yes
id
8d6a31b5-125f-4825-9a46-5d9d942c0c93 (old id 1106031)
date added to LUP
2008-08-01 11:08:23
date last changed
2017-02-22 11:44:24
@article{8d6a31b5-125f-4825-9a46-5d9d942c0c93,
  abstract     = {Mechanical properties of isolated smooth muscle strips from human urinary bladder were investigated in vitro. Bladder tissue was obtained from tumour-free wall regions of bladders from male patients undergoing cystectomy for bladder carcinoma. In intact muscle strips, activated with high-K+ solution, half-maximal force occurred at about 0.9 mM extracellular [Ca2+]. The length-active force relation was determined and the muscle strips were fixed for light and electron microscopy at optimal length for active force (1o). The maximal active force per unit smooth muscle cross-sectional area was 208 +/- 49 mN/mm2, n = 6. Chemically skinned preparations were obtained by treatment with triton X-100. These preparations had a steep [Ca2+]-force relation in the micromolar range which was influenced by calmodulin. The skinned preparations could be maximally activated by irreversible thiophosphorylation of the regulatory light chains. The force-velocity relation was determined in the maximally activated skinned muscle at 22 degrees C at 0.51o. When the muscle was shortened by 10%, force was reduced by 35% whereas the maximal shortening velocity was little affected.},
  author       = {Malmqvist, Ulf and Arner, Anders and Uvelius, Bengt},
  issn         = {0001-6772},
  language     = {eng},
  number       = {4},
  pages        = {373--380},
  publisher    = {Wiley-Blackwell},
  series       = {Acta Physiologica Scandinavica},
  title        = {Mechanics and Ca(2+)-sensitivity of human detrusor muscle bundles studied in vitro},
  volume       = {143},
  year         = {1991},
}