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Lactate dehydrogenase activity and isoform distribution in normal and hypertrophic smooth muscle tissue from the rat

Malmqvist, Ulf LU ; Arner, Anders LU and Uvelius, Bengt LU (1991) In Pflügers Archiv 419(3-4). p.230-234
Abstract
The lactate dehydrogenase (LDH) activity and isoform distribution of LDH were investigated in tissue samples from the rat portal vein, aorta and urinary bladder. In addition, samples were obtained from hypertrophic urinary bladder. The total LDH activity per unit smooth muscle volume was higher in the urinary bladder compared to that in portal vein and aorta. Five LDH isoforms, reflecting different combinations of the two polypeptide chains denoted H and M, could be separated by agarose gel electrophoresis. The aorta contained more of the H form compared to the portal vein and urinary bladder. This difference suggests that the aorta, which is a slow smooth muscle, is more adapted for aerobic metabolism than the faster muscles of portal... (More)
The lactate dehydrogenase (LDH) activity and isoform distribution of LDH were investigated in tissue samples from the rat portal vein, aorta and urinary bladder. In addition, samples were obtained from hypertrophic urinary bladder. The total LDH activity per unit smooth muscle volume was higher in the urinary bladder compared to that in portal vein and aorta. Five LDH isoforms, reflecting different combinations of the two polypeptide chains denoted H and M, could be separated by agarose gel electrophoresis. The aorta contained more of the H form compared to the portal vein and urinary bladder. This difference suggests that the aorta, which is a slow smooth muscle, is more adapted for aerobic metabolism than the faster muscles of portal vein and urinary bladder. In the hypertrophic urinary bladder a shift in LDH isoform pattern towards less of the H form was found, which correlates with a better maintenance of contraction in anoxia in this type of hypertrophic smooth muscle. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Lactate dehydrohgenase, Smooth muscle, Isoforms, Hypertrophy, Rat urinary bladder, Rat portal vein, Rat arota
in
Pflügers Archiv
volume
419
issue
3-4
pages
230 - 234
publisher
Springer
external identifiers
  • pmid:1745598
  • scopus:0026012306
ISSN
0031-6768
DOI
10.1007/BF00371100
language
English
LU publication?
yes
id
488a955f-75c3-4860-8489-d9200f83bb96 (old id 1106034)
date added to LUP
2008-08-01 11:11:04
date last changed
2017-02-22 11:44:28
@article{488a955f-75c3-4860-8489-d9200f83bb96,
  abstract     = {The lactate dehydrogenase (LDH) activity and isoform distribution of LDH were investigated in tissue samples from the rat portal vein, aorta and urinary bladder. In addition, samples were obtained from hypertrophic urinary bladder. The total LDH activity per unit smooth muscle volume was higher in the urinary bladder compared to that in portal vein and aorta. Five LDH isoforms, reflecting different combinations of the two polypeptide chains denoted H and M, could be separated by agarose gel electrophoresis. The aorta contained more of the H form compared to the portal vein and urinary bladder. This difference suggests that the aorta, which is a slow smooth muscle, is more adapted for aerobic metabolism than the faster muscles of portal vein and urinary bladder. In the hypertrophic urinary bladder a shift in LDH isoform pattern towards less of the H form was found, which correlates with a better maintenance of contraction in anoxia in this type of hypertrophic smooth muscle.},
  author       = {Malmqvist, Ulf and Arner, Anders and Uvelius, Bengt},
  issn         = {0031-6768},
  keyword      = {Lactate dehydrohgenase,Smooth muscle,Isoforms,Hypertrophy,Rat urinary bladder,Rat portal vein,Rat arota},
  language     = {eng},
  number       = {3-4},
  pages        = {230--234},
  publisher    = {Springer},
  series       = {Pflügers Archiv},
  title        = {Lactate dehydrogenase activity and isoform distribution in normal and hypertrophic smooth muscle tissue from the rat},
  url          = {http://dx.doi.org/10.1007/BF00371100},
  volume       = {419},
  year         = {1991},
}