Structure and expression of the gene encoding cystatin D, a novel human cysteine proteinase inhibitor
(1991) In Journal of Biological Chemistry 266(30). p.20538-20543- Abstract
- A new member of the human cystatin multigene family has been cloned from a genomic library using a cystatin C cDNA probe. The complete nucleotide sequence of a 4.3-kilobase DNA segment, containing a complete gene with structure very similar to those of known Family 2 cystatin genes, was determined. The novel gene, called CST4, is composed of three exons and two introns. It contains the coding information for a protein of 142 amino acid residues, which has been tentatively called cystatin D. The deduced amino acid sequence includes a putative signal peptide and presents 51-55% identical residues with the sequences of either cystatin C or the secretory gland cystatins S, SN, or SA. The cystatin D sequence contains all regions of relevance... (More)
- A new member of the human cystatin multigene family has been cloned from a genomic library using a cystatin C cDNA probe. The complete nucleotide sequence of a 4.3-kilobase DNA segment, containing a complete gene with structure very similar to those of known Family 2 cystatin genes, was determined. The novel gene, called CST4, is composed of three exons and two introns. It contains the coding information for a protein of 142 amino acid residues, which has been tentatively called cystatin D. The deduced amino acid sequence includes a putative signal peptide and presents 51-55% identical residues with the sequences of either cystatin C or the secretory gland cystatins S, SN, or SA. The cystatin D sequence contains all regions of relevance for cysteine proteinase inhibitory activity and also the 4 cysteine residues that form disulfide bridges in the other members of cystatin Family 2. Northern blot analysis revealed that the cystatin D gene is expressed in parotid gland but not in seminal vesicle, prostate, epididymis, testis, ovary, placenta, thyroid, gastric corpus, small intestine, liver, or gall-bladder tissue. This tissue-restricted expression is in marked contrast with the wider distribution of all the other Family 2 cystatins, since cystatin C is expressed in all these tissues and the secretory gland cystatins are present in saliva, seminal plasma, and tears. Cystatin D, being the first described member of a third subfamily within the cystatin Family 2, thus appears to have a distinct function in the body in contrast to other cystatins. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1106111
- author
- Freije, José P ; Abrahamson, Magnus LU ; Olafsson, Isleifur ; Velasco, Gloria ; Grubb, Anders LU and Lopez-Otin, Carlos
- organization
- publishing date
- 1991
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biological Chemistry
- volume
- 266
- issue
- 30
- pages
- 20538 - 20543
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- scopus:0025757378
- ISSN
- 1083-351X
- language
- English
- LU publication?
- yes
- id
- b8ff40f8-dd21-4ef9-9ff1-55bb47ae34db (old id 1106111)
- alternative location
- http://www.jbc.org/cgi/reprint/266/30/20538
- date added to LUP
- 2016-04-01 12:33:26
- date last changed
- 2021-02-07 05:23:56
@article{b8ff40f8-dd21-4ef9-9ff1-55bb47ae34db, abstract = {{A new member of the human cystatin multigene family has been cloned from a genomic library using a cystatin C cDNA probe. The complete nucleotide sequence of a 4.3-kilobase DNA segment, containing a complete gene with structure very similar to those of known Family 2 cystatin genes, was determined. The novel gene, called CST4, is composed of three exons and two introns. It contains the coding information for a protein of 142 amino acid residues, which has been tentatively called cystatin D. The deduced amino acid sequence includes a putative signal peptide and presents 51-55% identical residues with the sequences of either cystatin C or the secretory gland cystatins S, SN, or SA. The cystatin D sequence contains all regions of relevance for cysteine proteinase inhibitory activity and also the 4 cysteine residues that form disulfide bridges in the other members of cystatin Family 2. Northern blot analysis revealed that the cystatin D gene is expressed in parotid gland but not in seminal vesicle, prostate, epididymis, testis, ovary, placenta, thyroid, gastric corpus, small intestine, liver, or gall-bladder tissue. This tissue-restricted expression is in marked contrast with the wider distribution of all the other Family 2 cystatins, since cystatin C is expressed in all these tissues and the secretory gland cystatins are present in saliva, seminal plasma, and tears. Cystatin D, being the first described member of a third subfamily within the cystatin Family 2, thus appears to have a distinct function in the body in contrast to other cystatins.}}, author = {{Freije, José P and Abrahamson, Magnus and Olafsson, Isleifur and Velasco, Gloria and Grubb, Anders and Lopez-Otin, Carlos}}, issn = {{1083-351X}}, language = {{eng}}, number = {{30}}, pages = {{20538--20543}}, publisher = {{American Society for Biochemistry and Molecular Biology}}, series = {{Journal of Biological Chemistry}}, title = {{Structure and expression of the gene encoding cystatin D, a novel human cysteine proteinase inhibitor}}, url = {{http://www.jbc.org/cgi/reprint/266/30/20538}}, volume = {{266}}, year = {{1991}}, }