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Structure and expression of the gene encoding cystatin D, a novel human cysteine proteinase inhibitor

Freije, José P; Abrahamson, Magnus LU ; Olafsson, Isleifur; Velasco, Gloria; Grubb, Anders LU and Lopez-Otin, Carlos (1991) In Journal of Biological Chemistry 266(30). p.20538-20543
Abstract
A new member of the human cystatin multigene family has been cloned from a genomic library using a cystatin C cDNA probe. The complete nucleotide sequence of a 4.3-kilobase DNA segment, containing a complete gene with structure very similar to those of known Family 2 cystatin genes, was determined. The novel gene, called CST4, is composed of three exons and two introns. It contains the coding information for a protein of 142 amino acid residues, which has been tentatively called cystatin D. The deduced amino acid sequence includes a putative signal peptide and presents 51-55% identical residues with the sequences of either cystatin C or the secretory gland cystatins S, SN, or SA. The cystatin D sequence contains all regions of relevance... (More)
A new member of the human cystatin multigene family has been cloned from a genomic library using a cystatin C cDNA probe. The complete nucleotide sequence of a 4.3-kilobase DNA segment, containing a complete gene with structure very similar to those of known Family 2 cystatin genes, was determined. The novel gene, called CST4, is composed of three exons and two introns. It contains the coding information for a protein of 142 amino acid residues, which has been tentatively called cystatin D. The deduced amino acid sequence includes a putative signal peptide and presents 51-55% identical residues with the sequences of either cystatin C or the secretory gland cystatins S, SN, or SA. The cystatin D sequence contains all regions of relevance for cysteine proteinase inhibitory activity and also the 4 cysteine residues that form disulfide bridges in the other members of cystatin Family 2. Northern blot analysis revealed that the cystatin D gene is expressed in parotid gland but not in seminal vesicle, prostate, epididymis, testis, ovary, placenta, thyroid, gastric corpus, small intestine, liver, or gall-bladder tissue. This tissue-restricted expression is in marked contrast with the wider distribution of all the other Family 2 cystatins, since cystatin C is expressed in all these tissues and the secretory gland cystatins are present in saliva, seminal plasma, and tears. Cystatin D, being the first described member of a third subfamily within the cystatin Family 2, thus appears to have a distinct function in the body in contrast to other cystatins. (Less)
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organization
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Contribution to journal
publication status
published
subject
in
Journal of Biological Chemistry
volume
266
issue
30
pages
20538 - 20543
publisher
ASBMB
external identifiers
  • scopus:0025757378
ISSN
1083-351X
language
English
LU publication?
yes
id
b8ff40f8-dd21-4ef9-9ff1-55bb47ae34db (old id 1106111)
alternative location
http://www.jbc.org/cgi/reprint/266/30/20538
date added to LUP
2008-08-01 10:37:26
date last changed
2017-08-27 04:24:08
@article{b8ff40f8-dd21-4ef9-9ff1-55bb47ae34db,
  abstract     = {A new member of the human cystatin multigene family has been cloned from a genomic library using a cystatin C cDNA probe. The complete nucleotide sequence of a 4.3-kilobase DNA segment, containing a complete gene with structure very similar to those of known Family 2 cystatin genes, was determined. The novel gene, called CST4, is composed of three exons and two introns. It contains the coding information for a protein of 142 amino acid residues, which has been tentatively called cystatin D. The deduced amino acid sequence includes a putative signal peptide and presents 51-55% identical residues with the sequences of either cystatin C or the secretory gland cystatins S, SN, or SA. The cystatin D sequence contains all regions of relevance for cysteine proteinase inhibitory activity and also the 4 cysteine residues that form disulfide bridges in the other members of cystatin Family 2. Northern blot analysis revealed that the cystatin D gene is expressed in parotid gland but not in seminal vesicle, prostate, epididymis, testis, ovary, placenta, thyroid, gastric corpus, small intestine, liver, or gall-bladder tissue. This tissue-restricted expression is in marked contrast with the wider distribution of all the other Family 2 cystatins, since cystatin C is expressed in all these tissues and the secretory gland cystatins are present in saliva, seminal plasma, and tears. Cystatin D, being the first described member of a third subfamily within the cystatin Family 2, thus appears to have a distinct function in the body in contrast to other cystatins.},
  author       = {Freije, José P and Abrahamson, Magnus and Olafsson, Isleifur and Velasco, Gloria and Grubb, Anders and Lopez-Otin, Carlos},
  issn         = {1083-351X},
  language     = {eng},
  number       = {30},
  pages        = {20538--20543},
  publisher    = {ASBMB},
  series       = {Journal of Biological Chemistry},
  title        = {Structure and expression of the gene encoding cystatin D, a novel human cysteine proteinase inhibitor},
  volume       = {266},
  year         = {1991},
}