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Determination of allotypes G1m(f) and G1m(z) at the genomic level by subclass specific amplification of DNA and use of allele specific probes

Balbin, M; Grubb, Anders LU ; Abrahamson, Magnus LU and Grubb, R (1991) In Experimental and Clinical Immunogenetics 8(2). p.88-95
Abstract
Two oligonucleotide primers were used for selective enzymatic amplification of a DNA segment encoding a major portion of the first constant region domain (CH1) of the human IgG1 heavy chain. The selective amplification was confirmed by use of subclass-specific oligonucleotide probes. Two 15-mer oligonucleotides, hybridizing with the alleles for the allotypes G1m(f) and (z), respectively, could then be used for determination at the genomic level of these two truly allelic allotypes. Serum and DNA samples from 12 individuals, one of them with a considerable amount of anti-Gm(f) antibodies, were used for allotype assignment by classical serological methods and by the new method operating at the genomic level. The resulting classifications... (More)
Two oligonucleotide primers were used for selective enzymatic amplification of a DNA segment encoding a major portion of the first constant region domain (CH1) of the human IgG1 heavy chain. The selective amplification was confirmed by use of subclass-specific oligonucleotide probes. Two 15-mer oligonucleotides, hybridizing with the alleles for the allotypes G1m(f) and (z), respectively, could then be used for determination at the genomic level of these two truly allelic allotypes. Serum and DNA samples from 12 individuals, one of them with a considerable amount of anti-Gm(f) antibodies, were used for allotype assignment by classical serological methods and by the new method operating at the genomic level. The resulting classifications agreed completely, demonstrating the reliability of the new method. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Experimental and Clinical Immunogenetics
volume
8
issue
2
pages
88 - 95
publisher
Karger
external identifiers
  • scopus:0026337694
ISSN
0254-9670
language
English
LU publication?
yes
id
d1f0f32a-447a-4ba8-bdff-32850286ddab (old id 1106124)
date added to LUP
2008-08-01 10:48:35
date last changed
2017-08-20 04:31:15
@article{d1f0f32a-447a-4ba8-bdff-32850286ddab,
  abstract     = {Two oligonucleotide primers were used for selective enzymatic amplification of a DNA segment encoding a major portion of the first constant region domain (CH1) of the human IgG1 heavy chain. The selective amplification was confirmed by use of subclass-specific oligonucleotide probes. Two 15-mer oligonucleotides, hybridizing with the alleles for the allotypes G1m(f) and (z), respectively, could then be used for determination at the genomic level of these two truly allelic allotypes. Serum and DNA samples from 12 individuals, one of them with a considerable amount of anti-Gm(f) antibodies, were used for allotype assignment by classical serological methods and by the new method operating at the genomic level. The resulting classifications agreed completely, demonstrating the reliability of the new method.},
  author       = {Balbin, M and Grubb, Anders and Abrahamson, Magnus and Grubb, R},
  issn         = {0254-9670},
  language     = {eng},
  number       = {2},
  pages        = {88--95},
  publisher    = {Karger},
  series       = {Experimental and Clinical Immunogenetics},
  title        = {Determination of allotypes G1m(f) and G1m(z) at the genomic level by subclass specific amplification of DNA and use of allele specific probes},
  volume       = {8},
  year         = {1991},
}