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Image cytometric DNA analysis in human breast cancer analysis may add prognostic information in diploid cases with low S-phase fraction by flow cytometry

Baldetorp, Bo LU ; Fernö, Mårten LU ; Fallenius, Anders; Fallenius-Vecchi, Ghita; Idvall, Inger; Olsson, Håkan LU ; Sigurdsson, Helgi; Åkerman, Måns LU and Killander, Dick LU (1992) In Cytometry 13(6). p.577-585
Abstract
Measurements of DNA ploidy can be performed either with image cytometry (ICM) or flow cytometry (FCM); both methods provide independent prognostic information in primary breast cancer. The aim of the present investigation was to compare the two methods and to relate the findings to prognosis (median follow-up 42 months). Concordance in ploidy status (diploid, tetraploid, aneuploid) was obtained in 76% of the samples (168/222). When the fraction of S-phase cells (SPF) from FCM analysis was also taken into consideration, four different groups of samples were obtained (Flow I-IV), which were considered to correspond to the Auer classification (Auer I-IV) of DNA histograms obtained from image cytometry. Complete concordance between the two... (More)
Measurements of DNA ploidy can be performed either with image cytometry (ICM) or flow cytometry (FCM); both methods provide independent prognostic information in primary breast cancer. The aim of the present investigation was to compare the two methods and to relate the findings to prognosis (median follow-up 42 months). Concordance in ploidy status (diploid, tetraploid, aneuploid) was obtained in 76% of the samples (168/222). When the fraction of S-phase cells (SPF) from FCM analysis was also taken into consideration, four different groups of samples were obtained (Flow I-IV), which were considered to correspond to the Auer classification (Auer I-IV) of DNA histograms obtained from image cytometry. Complete concordance between the two techniques now was 70% (155/222). Samples classified as Flow I (diploid or near-diploid with low SPF) and Auer I had a distant metastasis rate of 3/60 (5%), as compared to 62/154 (40%) for all other combinations of the Flow and Auer classifications taken together. Thus, the only findings of prognostic importance were that some samples were Flow I but not Auer I, or vice versa. These two groups represent 17 (7.7%) and 14 (6.3%), respectively, of the total number of samples, and had frequencies of distant metastasis similar to those of the other high-risk groups, namely, 7/17 and 5/14, respectively. In a multivariate analysis, flow cytometric S-phase value was a stronger prognostic factor than either the Flow and Auer classification. We conclude that when routine FCM DNA analysis is used, diploid or near-diploid samples with a low S-phase value should be reanalyzed with ICM. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ploidy, proliferation, SPF, interphase, image cytometry, breast cancer, prognosis
in
Cytometry
volume
13
issue
6
pages
577 - 585
publisher
John Wiley & Sons
external identifiers
  • pmid:1451590
  • scopus:0026650564
ISSN
0196-4763
DOI
10.1002/cyto.990130605
language
English
LU publication?
yes
id
b915085d-165e-4070-b105-96f8d9c980cb (old id 1106265)
date added to LUP
2008-07-31 09:52:53
date last changed
2017-09-24 04:20:10
@article{b915085d-165e-4070-b105-96f8d9c980cb,
  abstract     = {Measurements of DNA ploidy can be performed either with image cytometry (ICM) or flow cytometry (FCM); both methods provide independent prognostic information in primary breast cancer. The aim of the present investigation was to compare the two methods and to relate the findings to prognosis (median follow-up 42 months). Concordance in ploidy status (diploid, tetraploid, aneuploid) was obtained in 76% of the samples (168/222). When the fraction of S-phase cells (SPF) from FCM analysis was also taken into consideration, four different groups of samples were obtained (Flow I-IV), which were considered to correspond to the Auer classification (Auer I-IV) of DNA histograms obtained from image cytometry. Complete concordance between the two techniques now was 70% (155/222). Samples classified as Flow I (diploid or near-diploid with low SPF) and Auer I had a distant metastasis rate of 3/60 (5%), as compared to 62/154 (40%) for all other combinations of the Flow and Auer classifications taken together. Thus, the only findings of prognostic importance were that some samples were Flow I but not Auer I, or vice versa. These two groups represent 17 (7.7%) and 14 (6.3%), respectively, of the total number of samples, and had frequencies of distant metastasis similar to those of the other high-risk groups, namely, 7/17 and 5/14, respectively. In a multivariate analysis, flow cytometric S-phase value was a stronger prognostic factor than either the Flow and Auer classification. We conclude that when routine FCM DNA analysis is used, diploid or near-diploid samples with a low S-phase value should be reanalyzed with ICM.},
  author       = {Baldetorp, Bo and Fernö, Mårten and Fallenius, Anders and Fallenius-Vecchi, Ghita and Idvall, Inger and Olsson, Håkan and Sigurdsson, Helgi and Åkerman, Måns and Killander, Dick},
  issn         = {0196-4763},
  keyword      = {Ploidy,proliferation,SPF,interphase,image cytometry,breast cancer,prognosis},
  language     = {eng},
  number       = {6},
  pages        = {577--585},
  publisher    = {John Wiley & Sons},
  series       = {Cytometry},
  title        = {Image cytometric DNA analysis in human breast cancer analysis may add prognostic information in diploid cases with low S-phase fraction by flow cytometry},
  url          = {http://dx.doi.org/10.1002/cyto.990130605},
  volume       = {13},
  year         = {1992},
}