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Mechanisms of adaptation to the effects of ethanol on activation of phospholipase C in NG 108-15 cells

Rodriguez, F D; Simonsson, Per LU ; Gustavsson, L and Alling, Christer LU (1992) In Neuropharmacology 31(11). p.1157-1164
Abstract
In this study the effect of different times of exposure to ethanol (1-7 days, 100 mM) on bradykinin and GTP(S)-stimulated activation of phospholipase C in NG 108-15 cells and on the binding of [3H]bradykinin to its receptors was investigated. Ethanol attenuated both agonist and GTP-analogue-induced hydrolysis of phosphoinositides for a period of up to 4 days of treatment, while exerting no effect on binding to bradykinin receptors. However, after 7 days of exposure to ethanol, the agonist-induced activation of phospholipase C was completely resistant to the inhibitory effects of alcohol. This finding correlated to a change in the affinity of the bradykinin receptor population after 7 days of treatment. The results indicate that... (More)
In this study the effect of different times of exposure to ethanol (1-7 days, 100 mM) on bradykinin and GTP(S)-stimulated activation of phospholipase C in NG 108-15 cells and on the binding of [3H]bradykinin to its receptors was investigated. Ethanol attenuated both agonist and GTP-analogue-induced hydrolysis of phosphoinositides for a period of up to 4 days of treatment, while exerting no effect on binding to bradykinin receptors. However, after 7 days of exposure to ethanol, the agonist-induced activation of phospholipase C was completely resistant to the inhibitory effects of alcohol. This finding correlated to a change in the affinity of the bradykinin receptor population after 7 days of treatment. The results indicate that bradykinin-induced breakdown of phosphatidylinositol 4,5-bisphosphate adapts to the effects of ethanol, after long-term treatment. Possible adaptative changes taking place at the level of the G protein(s), may induce a shift in the affinity of the receptor population and, consequently, serve as a compensatory mechanism to counteract the inhibitory effect of ethanol. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
NG 108-15 cell line, adaptation, inositol phosphates, bradykinin, G proteins, ethanol
in
Neuropharmacology
volume
31
issue
11
pages
1157 - 1164
publisher
Elsevier
external identifiers
  • pmid:1475023
  • scopus:0026494830
ISSN
1873-7064
DOI
10.1016/0028-3908(92)90012-E
language
English
LU publication?
yes
id
d7757200-0541-4647-9372-4045c249409b (old id 1106764)
date added to LUP
2008-08-12 09:21:21
date last changed
2017-08-06 03:45:51
@article{d7757200-0541-4647-9372-4045c249409b,
  abstract     = {In this study the effect of different times of exposure to ethanol (1-7 days, 100 mM) on bradykinin and GTP(S)-stimulated activation of phospholipase C in NG 108-15 cells and on the binding of [3H]bradykinin to its receptors was investigated. Ethanol attenuated both agonist and GTP-analogue-induced hydrolysis of phosphoinositides for a period of up to 4 days of treatment, while exerting no effect on binding to bradykinin receptors. However, after 7 days of exposure to ethanol, the agonist-induced activation of phospholipase C was completely resistant to the inhibitory effects of alcohol. This finding correlated to a change in the affinity of the bradykinin receptor population after 7 days of treatment. The results indicate that bradykinin-induced breakdown of phosphatidylinositol 4,5-bisphosphate adapts to the effects of ethanol, after long-term treatment. Possible adaptative changes taking place at the level of the G protein(s), may induce a shift in the affinity of the receptor population and, consequently, serve as a compensatory mechanism to counteract the inhibitory effect of ethanol.},
  author       = {Rodriguez, F D and Simonsson, Per and Gustavsson, L and Alling, Christer},
  issn         = {1873-7064},
  keyword      = {NG 108-15 cell line,adaptation,inositol phosphates,bradykinin,G proteins,ethanol},
  language     = {eng},
  number       = {11},
  pages        = {1157--1164},
  publisher    = {Elsevier},
  series       = {Neuropharmacology},
  title        = {Mechanisms of adaptation to the effects of ethanol on activation of phospholipase C in NG 108-15 cells},
  url          = {http://dx.doi.org/10.1016/0028-3908(92)90012-E},
  volume       = {31},
  year         = {1992},
}