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Desensitization of acetylcholine induced inositol 1,4,5-trisphosphate formation in neuroblastoma SH-SY5Y cells following repetitive acetylcholine stimulations

Larsson, Christer LU and Simonsson, Per LU (1993) In Neuroscience Letters 150(2). p.141-144
Abstract
In this study, the desensitization of acetylcholine-induced inositol 1,4,5-trisphosphate [I(1,4,5)P3] formation, upon short-time prestimulations, was investigated in cultures of human neuroblastoma SH-SY5Y cells. Four repeated stimulations for 10 seconds with 10 microM acetylcholine were necessary to induce a desensitization of the I(1,4,5)P3 formation. The desensitization was observed 4 hours after the initiation of repetitive stimulations. The same effect was obtained by a single prestimulation with 1 mM acetylcholine. Preincubation of the cells with phorbol 12-myristate 13-acetate (PMA) markedly down-regulated the acetylcholine-induced I(1,4,5)P3 formation. However, the protein kinase C (PKC) inhibitors H7 and staurosporine did not... (More)
In this study, the desensitization of acetylcholine-induced inositol 1,4,5-trisphosphate [I(1,4,5)P3] formation, upon short-time prestimulations, was investigated in cultures of human neuroblastoma SH-SY5Y cells. Four repeated stimulations for 10 seconds with 10 microM acetylcholine were necessary to induce a desensitization of the I(1,4,5)P3 formation. The desensitization was observed 4 hours after the initiation of repetitive stimulations. The same effect was obtained by a single prestimulation with 1 mM acetylcholine. Preincubation of the cells with phorbol 12-myristate 13-acetate (PMA) markedly down-regulated the acetylcholine-induced I(1,4,5)P3 formation. However, the protein kinase C (PKC) inhibitors H7 and staurosporine did not influence the desensitization induced by four repeated stimulations with 20 microM acetylcholine. These results indicate that the signal transduction can be desensitized following repeated stimulations with sub-maximal concentrations of receptor agonist and although activation of PKC can induce the same down-regulation, PKC is most likely not involved in the desensitization induced by repetitive acetylcholine-stimulations. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
SH-SY5Y neuroblastoma cell, Desensitization, Cholinergic receptor, Acetylcholine, Inositol 1, 4, 5-trisphosphate formation, Protein kinase C
in
Neuroscience Letters
volume
150
issue
2
pages
141 - 144
publisher
Elsevier
external identifiers
  • pmid:8385753
  • scopus:0027191891
ISSN
0304-3940
DOI
10.1016/0304-3940(93)90521-L
language
English
LU publication?
yes
id
71f50548-09bb-4dbd-814e-97b653f0f2bb (old id 1106955)
date added to LUP
2008-07-30 08:32:17
date last changed
2017-01-01 04:20:56
@article{71f50548-09bb-4dbd-814e-97b653f0f2bb,
  abstract     = {In this study, the desensitization of acetylcholine-induced inositol 1,4,5-trisphosphate [I(1,4,5)P3] formation, upon short-time prestimulations, was investigated in cultures of human neuroblastoma SH-SY5Y cells. Four repeated stimulations for 10 seconds with 10 microM acetylcholine were necessary to induce a desensitization of the I(1,4,5)P3 formation. The desensitization was observed 4 hours after the initiation of repetitive stimulations. The same effect was obtained by a single prestimulation with 1 mM acetylcholine. Preincubation of the cells with phorbol 12-myristate 13-acetate (PMA) markedly down-regulated the acetylcholine-induced I(1,4,5)P3 formation. However, the protein kinase C (PKC) inhibitors H7 and staurosporine did not influence the desensitization induced by four repeated stimulations with 20 microM acetylcholine. These results indicate that the signal transduction can be desensitized following repeated stimulations with sub-maximal concentrations of receptor agonist and although activation of PKC can induce the same down-regulation, PKC is most likely not involved in the desensitization induced by repetitive acetylcholine-stimulations.},
  author       = {Larsson, Christer and Simonsson, Per},
  issn         = {0304-3940},
  keyword      = {SH-SY5Y neuroblastoma cell,Desensitization,Cholinergic receptor,Acetylcholine,Inositol 1,4,5-trisphosphate formation,Protein kinase C},
  language     = {eng},
  number       = {2},
  pages        = {141--144},
  publisher    = {Elsevier},
  series       = {Neuroscience Letters},
  title        = {Desensitization of acetylcholine induced inositol 1,4,5-trisphosphate formation in neuroblastoma SH-SY5Y cells following repetitive acetylcholine stimulations},
  url          = {http://dx.doi.org/10.1016/0304-3940(93)90521-L},
  volume       = {150},
  year         = {1993},
}