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Lactate dehydrogenase activity and isoform distribution in the rat urinary bladder: effects of outlet obstruction and its removal

Polyanska, M; Arner, Anders LU ; Malmquist, U and Uvelius, Bengt LU (1993) In Journal of Urology 150(2 Pt 1). p.543-545
Abstract
The rat urinary bladder responded to infravesical obstruction by an increased weight. The weight began to increase after 3 days of obstruction. After 10 days the weight gain had become significant, but increased further with time. At 90 days the bladders weighed 12 times the initial weight. Lactate dehydrogenase (LDH) activity per unit bladder weight increased with the duration of the obstruction. The increase was significant in the 90 day group. The isoform pattern of LDH was determined. For the control group the M3H form was dominant, with M4 second; M4 became the dominant isoform after 3 days of obstruction. The relative amount of M4 had increased by 80% after 90 days. In one group, which was first obstructed for 10 days and then had... (More)
The rat urinary bladder responded to infravesical obstruction by an increased weight. The weight began to increase after 3 days of obstruction. After 10 days the weight gain had become significant, but increased further with time. At 90 days the bladders weighed 12 times the initial weight. Lactate dehydrogenase (LDH) activity per unit bladder weight increased with the duration of the obstruction. The increase was significant in the 90 day group. The isoform pattern of LDH was determined. For the control group the M3H form was dominant, with M4 second; M4 became the dominant isoform after 3 days of obstruction. The relative amount of M4 had increased by 80% after 90 days. In one group, which was first obstructed for 10 days and then had the obstruction removed for 4 weeks, bladder weight and LDH isoform pattern were normal. We suggest that the changes in isoform pattern in the obstructed bladder reflect a metabolic adaptation to the prolonged voiding time and the increased detrusor pressure, with a consequently compromised oxygen supply, and that the changes are reversible if the outlet conditions are normalized. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Urology
volume
150
issue
2 Pt 1
pages
543 - 545
publisher
Lippincott Williams & Wilkins
external identifiers
  • pmid:8326597
  • scopus:0027283970
ISSN
1527-3792
language
English
LU publication?
yes
id
2fbe162b-24fe-4bcc-8d91-9f8f1bf11bc4 (old id 1107560)
date added to LUP
2008-07-31 11:38:26
date last changed
2017-02-22 11:44:35
@article{2fbe162b-24fe-4bcc-8d91-9f8f1bf11bc4,
  abstract     = {The rat urinary bladder responded to infravesical obstruction by an increased weight. The weight began to increase after 3 days of obstruction. After 10 days the weight gain had become significant, but increased further with time. At 90 days the bladders weighed 12 times the initial weight. Lactate dehydrogenase (LDH) activity per unit bladder weight increased with the duration of the obstruction. The increase was significant in the 90 day group. The isoform pattern of LDH was determined. For the control group the M3H form was dominant, with M4 second; M4 became the dominant isoform after 3 days of obstruction. The relative amount of M4 had increased by 80% after 90 days. In one group, which was first obstructed for 10 days and then had the obstruction removed for 4 weeks, bladder weight and LDH isoform pattern were normal. We suggest that the changes in isoform pattern in the obstructed bladder reflect a metabolic adaptation to the prolonged voiding time and the increased detrusor pressure, with a consequently compromised oxygen supply, and that the changes are reversible if the outlet conditions are normalized.},
  author       = {Polyanska, M and Arner, Anders and Malmquist, U and Uvelius, Bengt},
  issn         = {1527-3792},
  language     = {eng},
  number       = {2 Pt 1},
  pages        = {543--545},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Journal of Urology},
  title        = {Lactate dehydrogenase activity and isoform distribution in the rat urinary bladder: effects of outlet obstruction and its removal},
  volume       = {150},
  year         = {1993},
}