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Mechanisms of muscarinic receptor-stimulated expression of c-fos in SH-SY5Y cells

Larsson, Christer LU ; Gustavsson, L; Simonsson, Per LU ; Bergman, O and Alling, Christer LU (1994) In European Journal of Pharmacology 268(1). p.19-28
Abstract
In this study, the signal cascade transducing carbachol stimulation into c-fos expression in SH-SY5Y neuroblastoma cells was investigated. 1,2-Diacylglycerol formation and c-fos expression were mediated via stimulation of muscarinic M1 receptors and the first 5 min of receptor stimulation were critical for these events. Application of 1,2-dioctanoylglycerol induced c-fos expression and this, as well as carbachol-stimulated c-fos expression, was inhibited by protein kinase C inhibitors. Increasing the intracellular Ca2+ concentration had only small effects on c-fos expression. There was a dependency on extracellular Ca2+ for maximal c-fos expression and 1,2-diacylglycerol formation. The carbachol-stimulated c-fos expression was potentiated... (More)
In this study, the signal cascade transducing carbachol stimulation into c-fos expression in SH-SY5Y neuroblastoma cells was investigated. 1,2-Diacylglycerol formation and c-fos expression were mediated via stimulation of muscarinic M1 receptors and the first 5 min of receptor stimulation were critical for these events. Application of 1,2-dioctanoylglycerol induced c-fos expression and this, as well as carbachol-stimulated c-fos expression, was inhibited by protein kinase C inhibitors. Increasing the intracellular Ca2+ concentration had only small effects on c-fos expression. There was a dependency on extracellular Ca2+ for maximal c-fos expression and 1,2-diacylglycerol formation. The carbachol-stimulated c-fos expression was potentiated by application of the protein phosphatase inhibitor okadaic acid. These results demonstrate the importance of 1,2-diacylglycerol formation for muscarinic receptor-stimulated, protein kinase C-mediated c-fos expression in the SH-SY5Y cells and that this cascade is counteracted by an okadaic acid-sensitive protein phosphatase. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
European Journal of Pharmacology
volume
268
issue
1
pages
19 - 28
publisher
Elsevier
external identifiers
  • pmid:7925609
ISSN
1879-0712
language
English
LU publication?
yes
id
e8bce303-919f-4d93-a44b-1be22716a2da (old id 1107715)
date added to LUP
2008-07-22 14:07:12
date last changed
2016-04-15 19:35:03
@article{e8bce303-919f-4d93-a44b-1be22716a2da,
  abstract     = {In this study, the signal cascade transducing carbachol stimulation into c-fos expression in SH-SY5Y neuroblastoma cells was investigated. 1,2-Diacylglycerol formation and c-fos expression were mediated via stimulation of muscarinic M1 receptors and the first 5 min of receptor stimulation were critical for these events. Application of 1,2-dioctanoylglycerol induced c-fos expression and this, as well as carbachol-stimulated c-fos expression, was inhibited by protein kinase C inhibitors. Increasing the intracellular Ca2+ concentration had only small effects on c-fos expression. There was a dependency on extracellular Ca2+ for maximal c-fos expression and 1,2-diacylglycerol formation. The carbachol-stimulated c-fos expression was potentiated by application of the protein phosphatase inhibitor okadaic acid. These results demonstrate the importance of 1,2-diacylglycerol formation for muscarinic receptor-stimulated, protein kinase C-mediated c-fos expression in the SH-SY5Y cells and that this cascade is counteracted by an okadaic acid-sensitive protein phosphatase.},
  author       = {Larsson, Christer and Gustavsson, L and Simonsson, Per and Bergman, O and Alling, Christer},
  issn         = {1879-0712},
  language     = {eng},
  number       = {1},
  pages        = {19--28},
  publisher    = {Elsevier},
  series       = {European Journal of Pharmacology},
  title        = {Mechanisms of muscarinic receptor-stimulated expression of c-fos in SH-SY5Y cells},
  volume       = {268},
  year         = {1994},
}