Migration of human granulocytes in filters: effects of gravity and movable gradients of f-MLP
(1994) In Biorheology 31(6). p.617-630- Abstract
- The Boyden chamber technique for chemotaxis uses a mesh filter that constitutes a matrix for cell locomotion and, at the same time, creates a local restriction for convective fluid movements that allows the establishment of a diffusive concentration gradient of chemotactic substance in the filter. In the present study, the Boyden chamber was modified by the introduction of a filter sandwich that allowed cell migration both upwards and downwards and by the use of a fluid density gradient controlling cell buoyancy and mechanically supporting a movable chemotactic gradient. This method was used to study chemotaxis and random migration of human granulocytes under the influence of gravitational forces and movable gradients of f-MLP. The results... (More)
- The Boyden chamber technique for chemotaxis uses a mesh filter that constitutes a matrix for cell locomotion and, at the same time, creates a local restriction for convective fluid movements that allows the establishment of a diffusive concentration gradient of chemotactic substance in the filter. In the present study, the Boyden chamber was modified by the introduction of a filter sandwich that allowed cell migration both upwards and downwards and by the use of a fluid density gradient controlling cell buoyancy and mechanically supporting a movable chemotactic gradient. This method was used to study chemotaxis and random migration of human granulocytes under the influence of gravitational forces and movable gradients of f-MLP. The results show that gravity affected cell motion significantly during random migration but not during chemotaxis. The rate of chemotactic migration was dependent on the steepness of the spatio-temporal f-MLP gradients. A stationary spatial gradient produced less migration than a gradient that was slowly moved through the filter sandwich in a direction opposite to that of the cell migration. The presence of f-MLP at constant concentration caused a minor, statistically insignificant, increase of the rate of random migration. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1107760
- author
- Braide, M ; Ebrahimzadeh, P R ; Strid, K G and Bjursten, Lars Magnus LU
- organization
- publishing date
- 1994
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biorheology
- volume
- 31
- issue
- 6
- pages
- 617 - 630
- publisher
- IOS Press
- external identifiers
-
- pmid:7696636
- scopus:0028600067
- ISSN
- 0006-355X
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Bioimplant Research (013242910)
- id
- 71a2039b-579d-4873-92a0-ae0cbc4756a8 (old id 1107760)
- date added to LUP
- 2016-04-01 12:03:14
- date last changed
- 2021-01-03 04:54:00
@article{71a2039b-579d-4873-92a0-ae0cbc4756a8,
abstract = {{The Boyden chamber technique for chemotaxis uses a mesh filter that constitutes a matrix for cell locomotion and, at the same time, creates a local restriction for convective fluid movements that allows the establishment of a diffusive concentration gradient of chemotactic substance in the filter. In the present study, the Boyden chamber was modified by the introduction of a filter sandwich that allowed cell migration both upwards and downwards and by the use of a fluid density gradient controlling cell buoyancy and mechanically supporting a movable chemotactic gradient. This method was used to study chemotaxis and random migration of human granulocytes under the influence of gravitational forces and movable gradients of f-MLP. The results show that gravity affected cell motion significantly during random migration but not during chemotaxis. The rate of chemotactic migration was dependent on the steepness of the spatio-temporal f-MLP gradients. A stationary spatial gradient produced less migration than a gradient that was slowly moved through the filter sandwich in a direction opposite to that of the cell migration. The presence of f-MLP at constant concentration caused a minor, statistically insignificant, increase of the rate of random migration.}},
author = {{Braide, M and Ebrahimzadeh, P R and Strid, K G and Bjursten, Lars Magnus}},
issn = {{0006-355X}},
language = {{eng}},
number = {{6}},
pages = {{617--630}},
publisher = {{IOS Press}},
series = {{Biorheology}},
title = {{Migration of human granulocytes in filters: effects of gravity and movable gradients of f-MLP}},
volume = {{31}},
year = {{1994}},
}