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Migration of human granulocytes in filters: effects of gravity and movable gradients of f-MLP

Braide, M; Ebrahimzadeh, P R; Strid, K G and Bjursten, Lars Magnus LU (1994) In Biorheology 31(6). p.617-630
Abstract
The Boyden chamber technique for chemotaxis uses a mesh filter that constitutes a matrix for cell locomotion and, at the same time, creates a local restriction for convective fluid movements that allows the establishment of a diffusive concentration gradient of chemotactic substance in the filter. In the present study, the Boyden chamber was modified by the introduction of a filter sandwich that allowed cell migration both upwards and downwards and by the use of a fluid density gradient controlling cell buoyancy and mechanically supporting a movable chemotactic gradient. This method was used to study chemotaxis and random migration of human granulocytes under the influence of gravitational forces and movable gradients of f-MLP. The results... (More)
The Boyden chamber technique for chemotaxis uses a mesh filter that constitutes a matrix for cell locomotion and, at the same time, creates a local restriction for convective fluid movements that allows the establishment of a diffusive concentration gradient of chemotactic substance in the filter. In the present study, the Boyden chamber was modified by the introduction of a filter sandwich that allowed cell migration both upwards and downwards and by the use of a fluid density gradient controlling cell buoyancy and mechanically supporting a movable chemotactic gradient. This method was used to study chemotaxis and random migration of human granulocytes under the influence of gravitational forces and movable gradients of f-MLP. The results show that gravity affected cell motion significantly during random migration but not during chemotaxis. The rate of chemotactic migration was dependent on the steepness of the spatio-temporal f-MLP gradients. A stationary spatial gradient produced less migration than a gradient that was slowly moved through the filter sandwich in a direction opposite to that of the cell migration. The presence of f-MLP at constant concentration caused a minor, statistically insignificant, increase of the rate of random migration. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biorheology
volume
31
issue
6
pages
617 - 630
publisher
IOS Press
external identifiers
  • pmid:7696636
  • scopus:0028600067
ISSN
0006-355X
language
English
LU publication?
yes
id
71a2039b-579d-4873-92a0-ae0cbc4756a8 (old id 1107760)
date added to LUP
2008-07-22 14:48:28
date last changed
2017-01-01 04:47:32
@article{71a2039b-579d-4873-92a0-ae0cbc4756a8,
  abstract     = {The Boyden chamber technique for chemotaxis uses a mesh filter that constitutes a matrix for cell locomotion and, at the same time, creates a local restriction for convective fluid movements that allows the establishment of a diffusive concentration gradient of chemotactic substance in the filter. In the present study, the Boyden chamber was modified by the introduction of a filter sandwich that allowed cell migration both upwards and downwards and by the use of a fluid density gradient controlling cell buoyancy and mechanically supporting a movable chemotactic gradient. This method was used to study chemotaxis and random migration of human granulocytes under the influence of gravitational forces and movable gradients of f-MLP. The results show that gravity affected cell motion significantly during random migration but not during chemotaxis. The rate of chemotactic migration was dependent on the steepness of the spatio-temporal f-MLP gradients. A stationary spatial gradient produced less migration than a gradient that was slowly moved through the filter sandwich in a direction opposite to that of the cell migration. The presence of f-MLP at constant concentration caused a minor, statistically insignificant, increase of the rate of random migration.},
  author       = {Braide, M and Ebrahimzadeh, P R and Strid, K G and Bjursten, Lars Magnus},
  issn         = {0006-355X},
  language     = {eng},
  number       = {6},
  pages        = {617--630},
  publisher    = {IOS Press},
  series       = {Biorheology},
  title        = {Migration of human granulocytes in filters: effects of gravity and movable gradients of f-MLP},
  volume       = {31},
  year         = {1994},
}