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An in vivo study of the effect of 5-HT and sympathetic nerves on transferrin and transthyretin mRNA expression in rat choroid plexus and meninges

Blay, Pilar; Nilsson, Christer LU ; Hansson, Stefan; Owman, Christer LU ; Aldred, Angela R and Schreiber, Gerhard (1994) In Brain Research 662(1-2). p.148-154
Abstract
Brain expression of transferrin (Tf) and transthyretin (TTR) mRNA has been demonstrated in different species, TTR being found only in the choroid plexus. We report here that both these mRNAs are also expressed in the meninges. In vitro studies have shown that Tf secretion by the rat choroid plexus is stimulated by 5-hydroxytryptamine (5-HT) while sympathetic nerves regulate different transport functions in the same tissue. We have used various in vivo models to study the neuroendocrine regulation of Tf and TTR mRNA expression in the choroid plexus and meninges. Destruction of the serotonergic nerves in the brain by either raphe nuclei lesion or intraventricular injections of 5,7-dihydroxytryptamine (5,7-DHT), which both decreased brain... (More)
Brain expression of transferrin (Tf) and transthyretin (TTR) mRNA has been demonstrated in different species, TTR being found only in the choroid plexus. We report here that both these mRNAs are also expressed in the meninges. In vitro studies have shown that Tf secretion by the rat choroid plexus is stimulated by 5-hydroxytryptamine (5-HT) while sympathetic nerves regulate different transport functions in the same tissue. We have used various in vivo models to study the neuroendocrine regulation of Tf and TTR mRNA expression in the choroid plexus and meninges. Destruction of the serotonergic nerves in the brain by either raphe nuclei lesion or intraventricular injections of 5,7-dihydroxytryptamine (5,7-DHT), which both decreased brain 5-HT levels significantly, did not affect Tf or TTR mRNA levels in choroid plexus and meninges, but increased TTR mRNA in liver. Intraventricular injection of 10 or 100 pmol 5-HT did not change the expression of these proteins in any of the tissues studied. Removal of the sympathetic innervation to the choroid plexus by cervical sympathectomy did not affect Tf or TTR mRNA levels in choroid plexus and liver, nor the incorporation of radioactive leucine into protein in various parts of the brain. In conclusion, our results do not support a regulatory role in vivo for neuronally derived 5-HT or sympathetic nerve activity on Tf and TTR mRNA expression in rat choroid plexus and meninges. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Transferrin, Transthyretin, 5-Hydroxytryptamine, Choroid plexus, Meninges, Sympathetic nerve, Raphe nuclei, Cerebrospinal fluid
in
Brain Research
volume
662
issue
1-2
pages
148 - 154
publisher
Elsevier
external identifiers
  • pmid:7859068
  • scopus:0027946187
ISSN
1872-6240
DOI
10.1016/0006-8993(94)90807-9
language
English
LU publication?
yes
id
8f450b0c-458c-4e7d-9aba-6bb68887b86b (old id 1108323)
date added to LUP
2008-07-23 17:13:54
date last changed
2017-01-01 04:20:21
@article{8f450b0c-458c-4e7d-9aba-6bb68887b86b,
  abstract     = {Brain expression of transferrin (Tf) and transthyretin (TTR) mRNA has been demonstrated in different species, TTR being found only in the choroid plexus. We report here that both these mRNAs are also expressed in the meninges. In vitro studies have shown that Tf secretion by the rat choroid plexus is stimulated by 5-hydroxytryptamine (5-HT) while sympathetic nerves regulate different transport functions in the same tissue. We have used various in vivo models to study the neuroendocrine regulation of Tf and TTR mRNA expression in the choroid plexus and meninges. Destruction of the serotonergic nerves in the brain by either raphe nuclei lesion or intraventricular injections of 5,7-dihydroxytryptamine (5,7-DHT), which both decreased brain 5-HT levels significantly, did not affect Tf or TTR mRNA levels in choroid plexus and meninges, but increased TTR mRNA in liver. Intraventricular injection of 10 or 100 pmol 5-HT did not change the expression of these proteins in any of the tissues studied. Removal of the sympathetic innervation to the choroid plexus by cervical sympathectomy did not affect Tf or TTR mRNA levels in choroid plexus and liver, nor the incorporation of radioactive leucine into protein in various parts of the brain. In conclusion, our results do not support a regulatory role in vivo for neuronally derived 5-HT or sympathetic nerve activity on Tf and TTR mRNA expression in rat choroid plexus and meninges.},
  author       = {Blay, Pilar and Nilsson, Christer and Hansson, Stefan and Owman, Christer and Aldred, Angela R and Schreiber, Gerhard},
  issn         = {1872-6240},
  keyword      = {Transferrin,Transthyretin,5-Hydroxytryptamine,Choroid plexus,Meninges,Sympathetic nerve,Raphe nuclei,Cerebrospinal fluid},
  language     = {eng},
  number       = {1-2},
  pages        = {148--154},
  publisher    = {Elsevier},
  series       = {Brain Research},
  title        = {An in vivo study of the effect of 5-HT and sympathetic nerves on transferrin and transthyretin mRNA expression in rat choroid plexus and meninges},
  url          = {http://dx.doi.org/10.1016/0006-8993(94)90807-9},
  volume       = {662},
  year         = {1994},
}