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Essential role of phosphatidylinositol 3-kinase in insulin-induced activation and phosphorylation of the cGMP-inhibited cAMP phosphodiesterase in rat adipocytes. Studies using the selective inhibitor wortmannin

Rahn, Tova; Ridderstråle, Martin LU ; Törnqvist, Hans; Manganiello, Vincent; Fredrikson, Gudrun; Belfrage, Per LU and Degerman, Eva LU (1994) In FEBS Letters 350(2-3). p.314-318
Abstract
Incubation of rat adipocytes with wortmannin, a potent and selective phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor, completely blocked the antilipolytic action of insulin (IC50 = 100 nM), the insulin-induced activation and phosphorylation of cGMP-inhibited cAMP phosphodiesterase (cGI-PDE) as well as the activation of the insulin-stimulated cGI-PDE kinase (IC50 = 10-30 nM). No direct effects of the inhibitor on the insulin-stimulated cGI-PDE kinase, the cGI-PDE and the hormone-sensitive lipase were observed. These data suggest that activation of PI 3-kinase upstream of the insulin-stimulated cGI-PDE kinase in the antilipolytic insulin signalchain has an essential role for insulin-induced cGI-PDE activation/phosphorylation and... (More)
Incubation of rat adipocytes with wortmannin, a potent and selective phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor, completely blocked the antilipolytic action of insulin (IC50 = 100 nM), the insulin-induced activation and phosphorylation of cGMP-inhibited cAMP phosphodiesterase (cGI-PDE) as well as the activation of the insulin-stimulated cGI-PDE kinase (IC50 = 10-30 nM). No direct effects of the inhibitor on the insulin-stimulated cGI-PDE kinase, the cGI-PDE and the hormone-sensitive lipase were observed. These data suggest that activation of PI 3-kinase upstream of the insulin-stimulated cGI-PDE kinase in the antilipolytic insulin signalchain has an essential role for insulin-induced cGI-PDE activation/phosphorylation and anti-lipolysis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Insulin, Phosphatidylinositol 3-kinase, Inhibitor, Adipocyte, Lipolysis, cGMP-inhibited cAMP phosphodiesterase
in
FEBS Letters
volume
350
issue
2-3
pages
314 - 318
publisher
Wiley-Blackwell
external identifiers
  • pmid:8070584
  • scopus:0028018097
ISSN
1873-3468
DOI
10.1016/0014-5793(94)00797-7
language
English
LU publication?
yes
id
678df2e0-d96f-4304-b64d-729f47e3bcb2 (old id 1108414)
date added to LUP
2008-07-24 09:53:36
date last changed
2017-08-06 04:23:38
@article{678df2e0-d96f-4304-b64d-729f47e3bcb2,
  abstract     = {Incubation of rat adipocytes with wortmannin, a potent and selective phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor, completely blocked the antilipolytic action of insulin (IC50 = 100 nM), the insulin-induced activation and phosphorylation of cGMP-inhibited cAMP phosphodiesterase (cGI-PDE) as well as the activation of the insulin-stimulated cGI-PDE kinase (IC50 = 10-30 nM). No direct effects of the inhibitor on the insulin-stimulated cGI-PDE kinase, the cGI-PDE and the hormone-sensitive lipase were observed. These data suggest that activation of PI 3-kinase upstream of the insulin-stimulated cGI-PDE kinase in the antilipolytic insulin signalchain has an essential role for insulin-induced cGI-PDE activation/phosphorylation and anti-lipolysis.},
  author       = {Rahn, Tova and Ridderstråle, Martin and Törnqvist, Hans and Manganiello, Vincent and Fredrikson, Gudrun and Belfrage, Per and Degerman, Eva},
  issn         = {1873-3468},
  keyword      = {Insulin,Phosphatidylinositol 3-kinase,Inhibitor,Adipocyte,Lipolysis,cGMP-inhibited cAMP phosphodiesterase},
  language     = {eng},
  number       = {2-3},
  pages        = {314--318},
  publisher    = {Wiley-Blackwell},
  series       = {FEBS Letters},
  title        = {Essential role of phosphatidylinositol 3-kinase in insulin-induced activation and phosphorylation of the cGMP-inhibited cAMP phosphodiesterase in rat adipocytes. Studies using the selective inhibitor wortmannin},
  url          = {http://dx.doi.org/10.1016/0014-5793(94)00797-7},
  volume       = {350},
  year         = {1994},
}