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No evidence for genomic imprinting of the human BCR gene

Fioretos, Thoas LU ; Heisterkamp, Nora and Groffen, John (1994) In Blood 83(12). p.3441-3444
Abstract
Chronic myeloid leukemias and 5% to 20% of acute lymphoid leukemias are characterized by the Philadelphia chromosome, a reciprocal chromosomal translocation, t(9;22)(q34;q11), generating BCR-ABL and ABL-BCR fusion genes. Cytogenetic studies have recently shown a preferential involvement of the paternally derived chromosome 9 and the maternally derived chromosome 22 in this translocation, indicating that imprinting might be involved in the formation or selection of the translocation. In this study, we have identified a BamHI polymorphism in the coding region of BCR exon 1, allowing us to investigate whether both BCR alleles are transcribed. By using a reverse transcriptase-polymerase chain reaction assay, we show that both BCR alleles are... (More)
Chronic myeloid leukemias and 5% to 20% of acute lymphoid leukemias are characterized by the Philadelphia chromosome, a reciprocal chromosomal translocation, t(9;22)(q34;q11), generating BCR-ABL and ABL-BCR fusion genes. Cytogenetic studies have recently shown a preferential involvement of the paternally derived chromosome 9 and the maternally derived chromosome 22 in this translocation, indicating that imprinting might be involved in the formation or selection of the translocation. In this study, we have identified a BamHI polymorphism in the coding region of BCR exon 1, allowing us to investigate whether both BCR alleles are transcribed. By using a reverse transcriptase-polymerase chain reaction assay, we show that both BCR alleles are expressed in the peripheral blood cells of normal individuals. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Blood
volume
83
issue
12
pages
3441 - 3444
publisher
American Society of Hematology
external identifiers
  • pmid:8204871
  • scopus:0028319233
ISSN
1528-0020
language
English
LU publication?
yes
id
8e10e187-47e9-4ad2-bacc-05736ab71e5c (old id 1108692)
alternative location
http://bloodjournal.hematologylibrary.org/cgi/reprint/83/12/3441
date added to LUP
2008-07-24 14:51:23
date last changed
2017-01-01 04:45:50
@article{8e10e187-47e9-4ad2-bacc-05736ab71e5c,
  abstract     = {Chronic myeloid leukemias and 5% to 20% of acute lymphoid leukemias are characterized by the Philadelphia chromosome, a reciprocal chromosomal translocation, t(9;22)(q34;q11), generating BCR-ABL and ABL-BCR fusion genes. Cytogenetic studies have recently shown a preferential involvement of the paternally derived chromosome 9 and the maternally derived chromosome 22 in this translocation, indicating that imprinting might be involved in the formation or selection of the translocation. In this study, we have identified a BamHI polymorphism in the coding region of BCR exon 1, allowing us to investigate whether both BCR alleles are transcribed. By using a reverse transcriptase-polymerase chain reaction assay, we show that both BCR alleles are expressed in the peripheral blood cells of normal individuals.},
  author       = {Fioretos, Thoas and Heisterkamp, Nora and Groffen, John},
  issn         = {1528-0020},
  language     = {eng},
  number       = {12},
  pages        = {3441--3444},
  publisher    = {American Society of Hematology},
  series       = {Blood},
  title        = {No evidence for genomic imprinting of the human BCR gene},
  volume       = {83},
  year         = {1994},
}